Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6167 and GFF6168 are separated by 22 nucleotides GFF6168 and GFF6169 overlap by 4 nucleotides GFF6169 and GFF6170 overlap by 4 nucleotides GFF6170 and GFF6171 overlap by 4 nucleotides
GFF6167 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 72,422 to 73,426
GFF6167
GFF6168 - Na(+) H(+) antiporter subunit G, at 73,449 to 73,820
GFF6168
GFF6169 - Na(+) H(+) antiporter subunit F, at 73,817 to 74,095
GFF6169
GFF6170 - Na(+) H(+) antiporter subunit E, at 74,092 to 74,583
GFF6170
GFF6171 - Na(+) H(+) antiporter subunit D, at 74,580 to 76,298
GFF6171
Position (kb)
74
75 Strain fitness (log2 ratio)
-2
-1
0
1 at 73.145 kb on + strand, within GFF6167 at 73.145 kb on + strand, within GFF6167 at 73.322 kb on + strand, within GFF6167 at 73.323 kb on - strand, within GFF6167 at 73.361 kb on + strand at 73.362 kb on - strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.424 kb on + strand at 73.425 kb on - strand at 73.425 kb on - strand at 73.425 kb on - strand at 73.637 kb on - strand, within GFF6168 at 73.637 kb on - strand, within GFF6168 at 73.908 kb on + strand, within GFF6169 at 73.908 kb on + strand, within GFF6169 at 73.908 kb on + strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.909 kb on - strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.965 kb on + strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 73.966 kb on - strand, within GFF6169 at 74.294 kb on - strand, within GFF6170 at 74.378 kb on + strand, within GFF6170 at 74.378 kb on + strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.379 kb on - strand, within GFF6170 at 74.662 kb on + strand at 74.880 kb on + strand, within GFF6171 at 74.880 kb on + strand, within GFF6171 at 74.880 kb on + strand, within GFF6171 at 74.881 kb on - strand, within GFF6171 at 74.881 kb on - strand, within GFF6171 at 74.881 kb on - strand, within GFF6171 at 75.055 kb on + strand, within GFF6171 at 75.235 kb on + strand, within GFF6171 at 75.235 kb on + strand, within GFF6171 at 75.235 kb on + strand, within GFF6171 at 75.235 kb on + strand, within GFF6171 at 75.236 kb on - strand, within GFF6171 at 75.236 kb on - strand, within GFF6171 at 75.472 kb on + strand, within GFF6171 at 75.472 kb on + strand, within GFF6171 at 75.472 kb on + strand, within GFF6171 at 75.472 kb on + strand, within GFF6171 at 75.473 kb on - strand, within GFF6171 at 75.473 kb on - strand, within GFF6171 at 75.473 kb on - strand, within GFF6171 at 75.473 kb on - strand, within GFF6171 at 75.473 kb on - strand, within GFF6171 at 75.532 kb on + strand, within GFF6171 at 75.532 kb on + strand, within GFF6171 at 75.532 kb on + strand, within GFF6171
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 73,145 + GFF6167 0.72 -0.4 73,145 + GFF6167 0.72 +0.1 73,322 + GFF6167 0.90 -0.0 73,323 - GFF6167 0.90 -0.4 73,361 + -0.5 73,362 - +0.5 73,424 + -0.1 73,424 + -1.5 73,424 + -0.5 73,424 + -0.2 73,424 + -0.5 73,424 + -0.6 73,424 + -0.4 73,424 + +0.7 73,424 + -0.1 73,424 + -1.1 73,424 + -0.6 73,425 - +0.6 73,425 - -0.8 73,425 - -0.3 73,637 - GFF6168 0.51 -1.5 73,637 - GFF6168 0.51 -0.7 73,908 + GFF6169 0.33 -0.4 73,908 + GFF6169 0.33 -0.0 73,908 + GFF6169 0.33 -2.0 73,909 - GFF6169 0.33 +0.4 73,909 - GFF6169 0.33 -1.7 73,909 - GFF6169 0.33 -1.5 73,909 - GFF6169 0.33 -1.2 73,909 - GFF6169 0.33 -1.2 73,909 - GFF6169 0.33 +0.2 73,965 + GFF6169 0.53 -0.5 73,965 + GFF6169 0.53 -0.7 73,965 + GFF6169 0.53 -1.3 73,965 + GFF6169 0.53 +1.3 73,965 + GFF6169 0.53 -0.8 73,965 + GFF6169 0.53 -0.8 73,965 + GFF6169 0.53 -0.6 73,965 + GFF6169 0.53 +0.3 73,965 + GFF6169 0.53 -1.5 73,966 - GFF6169 0.53 +0.1 73,966 - GFF6169 0.53 -0.8 73,966 - GFF6169 0.53 -2.7 73,966 - GFF6169 0.53 +0.4 74,294 - GFF6170 0.41 +0.8 74,378 + GFF6170 0.58 -0.0 74,378 + GFF6170 0.58 -0.3 74,379 - GFF6170 0.58 +0.3 74,379 - GFF6170 0.58 +1.6 74,379 - GFF6170 0.58 -0.0 74,379 - GFF6170 0.58 -0.3 74,379 - GFF6170 0.58 -0.3 74,662 + +0.3 74,880 + GFF6171 0.17 +0.4 74,880 + GFF6171 0.17 -0.0 74,880 + GFF6171 0.17 -0.2 74,881 - GFF6171 0.18 -0.3 74,881 - GFF6171 0.18 -0.3 74,881 - GFF6171 0.18 -0.4 75,055 + GFF6171 0.28 -0.4 75,235 + GFF6171 0.38 -1.4 75,235 + GFF6171 0.38 -0.5 75,235 + GFF6171 0.38 +0.1 75,235 + GFF6171 0.38 -1.2 75,236 - GFF6171 0.38 +0.6 75,236 - GFF6171 0.38 -0.2 75,472 + GFF6171 0.52 -0.3 75,472 + GFF6171 0.52 -0.9 75,472 + GFF6171 0.52 -0.7 75,472 + GFF6171 0.52 +0.0 75,473 - GFF6171 0.52 -0.8 75,473 - GFF6171 0.52 +0.2 75,473 - GFF6171 0.52 +1.1 75,473 - GFF6171 0.52 +0.7 75,473 - GFF6171 0.52 -1.2 75,532 + GFF6171 0.55 +0.6 75,532 + GFF6171 0.55 +0.6 75,532 + GFF6171 0.55 -2.0
Or see this region's nucleotide sequence