Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF60 and GFF61 overlap by 8 nucleotides GFF61 and GFF62 are separated by 16 nucleotides
GFF60 - GDP-L-fucose synthetase (EC 1.1.1.271), at 75,995 to 76,942
GFF60
GFF61 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 76,935 to 78,059
GFF61
GFF62 - hypothetical protein, at 78,076 to 79,422
GFF62
Position (kb) 76
77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 75.965 kb on - strand at 76.130 kb on + strand, within GFF60 at 76.130 kb on + strand, within GFF60 at 76.130 kb on + strand, within GFF60 at 76.131 kb on - strand, within GFF60 at 76.131 kb on - strand, within GFF60 at 76.175 kb on + strand, within GFF60 at 76.176 kb on - strand, within GFF60 at 76.193 kb on + strand, within GFF60 at 76.194 kb on - strand, within GFF60 at 76.283 kb on + strand, within GFF60 at 76.283 kb on + strand, within GFF60 at 76.283 kb on + strand, within GFF60 at 76.283 kb on + strand, within GFF60 at 76.284 kb on - strand, within GFF60 at 76.358 kb on + strand, within GFF60 at 76.359 kb on - strand, within GFF60 at 76.400 kb on + strand, within GFF60 at 76.400 kb on + strand, within GFF60 at 76.400 kb on + strand, within GFF60 at 76.436 kb on + strand, within GFF60 at 76.437 kb on - strand, within GFF60 at 76.464 kb on - strand, within GFF60 at 76.481 kb on + strand, within GFF60 at 76.481 kb on + strand, within GFF60 at 76.482 kb on - strand, within GFF60 at 76.482 kb on - strand, within GFF60 at 76.482 kb on - strand, within GFF60 at 76.601 kb on + strand, within GFF60 at 76.601 kb on + strand, within GFF60 at 76.659 kb on - strand, within GFF60 at 76.983 kb on + strand at 76.984 kb on - strand at 76.984 kb on - strand at 76.984 kb on - strand at 77.077 kb on - strand, within GFF61 at 77.187 kb on + strand, within GFF61 at 77.188 kb on - strand, within GFF61 at 77.322 kb on + strand, within GFF61 at 77.323 kb on - strand, within GFF61 at 77.323 kb on - strand, within GFF61 at 77.347 kb on - strand, within GFF61 at 77.392 kb on - strand, within GFF61 at 77.424 kb on + strand, within GFF61 at 77.424 kb on + strand, within GFF61 at 77.448 kb on + strand, within GFF61 at 77.449 kb on - strand, within GFF61 at 77.460 kb on + strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.469 kb on + strand, within GFF61 at 77.470 kb on - strand, within GFF61 at 77.634 kb on + strand, within GFF61 at 77.634 kb on + strand, within GFF61 at 77.682 kb on + strand, within GFF61 at 77.683 kb on - strand, within GFF61 at 77.683 kb on - strand, within GFF61 at 77.706 kb on + strand, within GFF61 at 78.247 kb on + strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 75,965 - +0.4 76,130 + GFF60 0.14 -0.2 76,130 + GFF60 0.14 -1.1 76,130 + GFF60 0.14 -1.1 76,131 - GFF60 0.14 +1.2 76,131 - GFF60 0.14 -0.3 76,175 + GFF60 0.19 -0.2 76,176 - GFF60 0.19 -0.5 76,193 + GFF60 0.21 +1.2 76,194 - GFF60 0.21 +0.2 76,283 + GFF60 0.30 -0.2 76,283 + GFF60 0.30 +1.0 76,283 + GFF60 0.30 -0.4 76,283 + GFF60 0.30 +0.5 76,284 - GFF60 0.30 -0.7 76,358 + GFF60 0.38 -0.7 76,359 - GFF60 0.38 -1.1 76,400 + GFF60 0.43 +0.1 76,400 + GFF60 0.43 -0.3 76,400 + GFF60 0.43 +0.2 76,436 + GFF60 0.47 +0.2 76,437 - GFF60 0.47 +0.6 76,464 - GFF60 0.49 -1.6 76,481 + GFF60 0.51 -0.2 76,481 + GFF60 0.51 -0.5 76,482 - GFF60 0.51 +0.9 76,482 - GFF60 0.51 -1.4 76,482 - GFF60 0.51 -0.0 76,601 + GFF60 0.64 +0.2 76,601 + GFF60 0.64 +0.4 76,659 - GFF60 0.70 -2.2 76,983 + -0.0 76,984 - -0.1 76,984 - +0.4 76,984 - +1.0 77,077 - GFF61 0.13 -0.1 77,187 + GFF61 0.22 +0.6 77,188 - GFF61 0.22 +0.2 77,322 + GFF61 0.34 +0.8 77,323 - GFF61 0.34 -0.1 77,323 - GFF61 0.34 -2.9 77,347 - GFF61 0.37 +0.2 77,392 - GFF61 0.41 +0.8 77,424 + GFF61 0.43 +0.2 77,424 + GFF61 0.43 +0.0 77,448 + GFF61 0.46 -0.9 77,449 - GFF61 0.46 +0.7 77,460 + GFF61 0.47 -0.8 77,461 - GFF61 0.47 -0.3 77,461 - GFF61 0.47 -0.5 77,461 - GFF61 0.47 +0.6 77,469 + GFF61 0.47 -0.2 77,470 - GFF61 0.48 -2.5 77,634 + GFF61 0.62 +0.5 77,634 + GFF61 0.62 +0.1 77,682 + GFF61 0.66 +0.3 77,683 - GFF61 0.66 +0.1 77,683 - GFF61 0.66 -0.4 77,706 + GFF61 0.69 +1.9 78,247 + GFF62 0.13 -1.1 78,356 - GFF62 0.21 +0.3 78,356 - GFF62 0.21 -0.5 78,356 - GFF62 0.21 +0.2 78,356 - GFF62 0.21 -0.4 78,356 - GFF62 0.21 -0.2 78,356 - GFF62 0.21 -0.3
Or see this region's nucleotide sequence