Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5853 and GFF5854 overlap by 4 nucleotides GFF5854 and GFF5855 are separated by 5 nucleotides
GFF5853 - Type I secretion system ATPase, LssB family LapB, at 32,166 to 34,292
GFF5853
GFF5854 - Type I secretion system, outer membrane component LapE, at 34,289 to 35,713
GFF5854
GFF5855 - T1SS secreted agglutinin RTX, at 35,719 to 55,560
GFF5855
Position (kb)
34
35
36 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 33.736 kb on + strand, within GFF5853 at 33.736 kb on + strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.871 kb on + strand, within GFF5853 at 33.871 kb on + strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 34.264 kb on + strand at 34.335 kb on + strand at 34.335 kb on + strand at 34.368 kb on + strand at 34.428 kb on + strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.482 kb on + strand, within GFF5854 at 34.482 kb on + strand, within GFF5854 at 34.482 kb on + strand, within GFF5854 at 34.689 kb on + strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 35.413 kb on - strand, within GFF5854 at 35.415 kb on + strand, within GFF5854 at 35.701 kb on + strand at 35.701 kb on + strand at 35.702 kb on - strand at 35.873 kb on + strand at 35.873 kb on + strand at 35.874 kb on - strand at 35.874 kb on - strand at 35.907 kb on - strand at 36.035 kb on + strand at 36.035 kb on + strand at 36.035 kb on + strand at 36.036 kb on - strand at 36.036 kb on - strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.336 kb on - strand at 36.336 kb on - strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.416 kb on + strand at 36.554 kb on + strand at 36.555 kb on - strand at 36.555 kb on - strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.591 kb on - strand at 36.593 kb on + strand at 36.593 kb on + strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 33,736 + GFF5853 0.74 +0.7 33,736 + GFF5853 0.74 -0.0 33,737 - GFF5853 0.74 -1.4 33,737 - GFF5853 0.74 +0.4 33,737 - GFF5853 0.74 -0.1 33,871 + GFF5853 0.80 +0.6 33,871 + GFF5853 0.80 -0.0 33,872 - GFF5853 0.80 +0.2 33,872 - GFF5853 0.80 -0.3 33,872 - GFF5853 0.80 -0.5 33,872 - GFF5853 0.80 -0.1 33,872 - GFF5853 0.80 -0.6 34,264 + -0.8 34,335 + +0.1 34,335 + -0.2 34,368 + +0.6 34,428 + +2.1 34,429 - -0.3 34,429 - -1.8 34,429 - +0.2 34,429 - -0.5 34,429 - +0.4 34,482 + GFF5854 0.14 -1.1 34,482 + GFF5854 0.14 +0.2 34,482 + GFF5854 0.14 -0.8 34,689 + GFF5854 0.28 +1.1 34,690 - GFF5854 0.28 +0.7 34,690 - GFF5854 0.28 +0.1 34,690 - GFF5854 0.28 -1.2 34,690 - GFF5854 0.28 +0.4 34,726 - GFF5854 0.31 -0.8 34,726 - GFF5854 0.31 -0.1 34,726 - GFF5854 0.31 -1.2 35,413 - GFF5854 0.79 -1.1 35,415 + GFF5854 0.79 +1.4 35,701 + +0.1 35,701 + +2.0 35,702 - +0.1 35,873 + -0.0 35,873 + +0.4 35,874 - +0.1 35,874 - -0.4 35,907 - -0.3 36,035 + +0.1 36,035 + -0.6 36,035 + -0.8 36,036 - +0.7 36,036 - +0.1 36,335 + +0.3 36,335 + -0.3 36,335 + +0.8 36,335 + -0.4 36,336 - +0.7 36,336 - +0.0 36,371 + -0.2 36,371 + +0.4 36,371 + -0.3 36,371 + -0.7 36,371 + +0.4 36,372 - +0.5 36,372 - -1.1 36,372 - +0.7 36,372 - -0.1 36,416 + +1.3 36,554 + +0.3 36,555 - -0.2 36,555 - -0.2 36,590 + -0.2 36,590 + +0.3 36,590 + -0.2 36,590 + -0.3 36,590 + -0.0 36,590 + +0.4 36,591 - -0.2 36,593 + -1.5 36,593 + -1.3 36,594 - +0.9 36,594 - +0.1 36,594 - -0.3 36,594 - -1.0 36,594 - +0.3 36,594 - +0.2 36,594 - -0.1 36,594 - +0.1
Or see this region's nucleotide sequence