Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5810 and GFF5811 overlap by 4 nucleotides GFF5811 and GFF5812 are separated by 18 nucleotides GFF5812 and GFF5813 overlap by 4 nucleotides
GFF5810 - Multidrug efflux system MdtABC-TolC, membrane fusion component MdtA, at 186,470 to 187,705
GFF5810
GFF5811 - Multidrug efflux system MdtABC-TolC, inner-membrane proton/drug antiporter MdtB-like, at 187,702 to 190,872
GFF5811
GFF5812 - DNA-binding heavy metal response regulator, at 190,891 to 191,631
GFF5812
GFF5813 - Heavy metal sensor histidine kinase, at 191,628 to 193,025
GFF5813
Position (kb) 187
188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1 at 186.914 kb on + strand, within GFF5810 at 186.914 kb on + strand, within GFF5810 at 186.915 kb on - strand, within GFF5810 at 187.026 kb on - strand, within GFF5810 at 187.026 kb on - strand, within GFF5810 at 187.254 kb on - strand, within GFF5810 at 187.475 kb on + strand, within GFF5810 at 187.475 kb on + strand, within GFF5810 at 187.475 kb on + strand, within GFF5810 at 187.476 kb on - strand, within GFF5810 at 187.476 kb on - strand, within GFF5810 at 187.476 kb on - strand, within GFF5810 at 187.476 kb on - strand, within GFF5810 at 187.604 kb on + strand at 187.604 kb on + strand at 187.604 kb on + strand at 187.604 kb on + strand at 187.604 kb on + strand at 187.604 kb on + strand at 188.436 kb on - strand, within GFF5811 at 188.592 kb on + strand, within GFF5811 at 188.593 kb on - strand, within GFF5811 at 188.869 kb on + strand, within GFF5811 at 188.881 kb on + strand, within GFF5811 at 188.969 kb on - strand, within GFF5811 at 189.011 kb on - strand, within GFF5811 at 189.049 kb on - strand, within GFF5811 at 189.241 kb on + strand, within GFF5811 at 189.242 kb on - strand, within GFF5811 at 189.284 kb on - strand, within GFF5811 at 189.284 kb on - strand, within GFF5811 at 189.550 kb on + strand, within GFF5811 at 189.551 kb on - strand, within GFF5811 at 189.551 kb on - strand, within GFF5811 at 189.568 kb on + strand, within GFF5811 at 189.569 kb on - strand, within GFF5811 at 189.724 kb on + strand, within GFF5811 at 189.979 kb on + strand, within GFF5811 at 189.980 kb on - strand, within GFF5811 at 190.043 kb on - strand, within GFF5811 at 190.117 kb on + strand, within GFF5811 at 190.118 kb on - strand, within GFF5811 at 190.309 kb on + strand, within GFF5811 at 190.309 kb on + strand, within GFF5811 at 190.340 kb on - strand, within GFF5811 at 190.369 kb on + strand, within GFF5811 at 190.370 kb on - strand, within GFF5811 at 190.431 kb on - strand, within GFF5811 at 190.580 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.768 kb on + strand at 190.768 kb on + strand at 190.769 kb on - strand at 190.769 kb on - strand at 190.792 kb on + strand at 190.966 kb on + strand, within GFF5812 at 191.573 kb on - strand at 191.748 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 186,914 + GFF5810 0.36 -1.0 186,914 + GFF5810 0.36 -0.3 186,915 - GFF5810 0.36 -0.1 187,026 - GFF5810 0.45 -0.3 187,026 - GFF5810 0.45 -0.2 187,254 - GFF5810 0.63 -0.6 187,475 + GFF5810 0.81 -0.0 187,475 + GFF5810 0.81 +1.2 187,475 + GFF5810 0.81 +0.1 187,476 - GFF5810 0.81 -0.8 187,476 - GFF5810 0.81 -0.0 187,476 - GFF5810 0.81 -0.1 187,476 - GFF5810 0.81 -0.7 187,604 + -1.3 187,604 + -1.0 187,604 + -0.5 187,604 + -0.3 187,604 + -0.0 187,604 + +0.3 188,436 - GFF5811 0.23 +1.0 188,592 + GFF5811 0.28 +0.3 188,593 - GFF5811 0.28 +0.4 188,869 + GFF5811 0.37 +0.7 188,881 + GFF5811 0.37 -0.8 188,969 - GFF5811 0.40 +0.4 189,011 - GFF5811 0.41 -0.3 189,049 - GFF5811 0.42 -0.2 189,241 + GFF5811 0.49 -0.2 189,242 - GFF5811 0.49 -0.0 189,284 - GFF5811 0.50 -0.0 189,284 - GFF5811 0.50 -0.0 189,550 + GFF5811 0.58 -0.0 189,551 - GFF5811 0.58 +0.8 189,551 - GFF5811 0.58 -0.8 189,568 + GFF5811 0.59 -0.4 189,569 - GFF5811 0.59 -0.2 189,724 + GFF5811 0.64 -1.0 189,979 + GFF5811 0.72 -0.3 189,980 - GFF5811 0.72 -0.5 190,043 - GFF5811 0.74 +0.4 190,117 + GFF5811 0.76 +0.1 190,118 - GFF5811 0.76 -0.2 190,309 + GFF5811 0.82 +0.1 190,309 + GFF5811 0.82 +0.2 190,340 - GFF5811 0.83 -0.7 190,369 + GFF5811 0.84 -2.1 190,370 - GFF5811 0.84 -0.4 190,431 - GFF5811 0.86 -0.3 190,580 - -0.5 190,763 - +1.2 190,763 - +1.6 190,763 - +0.7 190,763 - -0.3 190,768 + +0.4 190,768 + -0.6 190,769 - +0.3 190,769 - -0.0 190,792 + -0.8 190,966 + GFF5812 0.10 -0.4 191,573 - -0.9 191,748 + -0.8
Or see this region's nucleotide sequence