Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5736 and GFF5737 are separated by 83 nucleotides GFF5737 and GFF5738 overlap by 4 nucleotides GFF5738 and GFF5739 overlap by 4 nucleotides
GFF5736 - Flagellar hook-associated protein FlgL, at 110,545 to 111,756
GFF5736
GFF5737 - 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphat e synthase I alpha (EC 2.5.1.54), at 111,840 to 112,964
GFF5737
GFF5738 - Flagellar biosynthesis protein FliR, at 112,961 to 113,758
GFF5738
GFF5739 - Flagellar biosynthesis protein FliQ, at 113,755 to 114,024
GFF5739
Position (kb) 111
112
113 Strain fitness (log2 ratio) -1
0
1 at 111.010 kb on + strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.130 kb on - strand, within GFF5736 at 111.238 kb on + strand, within GFF5736 at 111.238 kb on + strand, within GFF5736 at 111.239 kb on - strand, within GFF5736 at 111.239 kb on - strand, within GFF5736 at 111.289 kb on + strand, within GFF5736 at 111.290 kb on - strand, within GFF5736 at 111.625 kb on + strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.749 kb on - strand at 112.219 kb on - strand, within GFF5737 at 112.219 kb on - strand, within GFF5737 at 113.403 kb on + strand, within GFF5738 at 113.442 kb on + strand, within GFF5738 at 113.443 kb on - strand, within GFF5738 at 113.798 kb on + strand, within GFF5739 at 113.799 kb on - strand, within GFF5739
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 111,010 + GFF5736 0.38 +0.9 111,011 - GFF5736 0.38 -1.3 111,011 - GFF5736 0.38 -0.3 111,011 - GFF5736 0.38 +0.1 111,011 - GFF5736 0.38 +0.8 111,130 - GFF5736 0.48 -0.3 111,238 + GFF5736 0.57 -0.0 111,238 + GFF5736 0.57 -0.9 111,239 - GFF5736 0.57 +1.0 111,239 - GFF5736 0.57 -0.2 111,289 + GFF5736 0.61 +0.2 111,290 - GFF5736 0.61 +0.6 111,625 + GFF5736 0.89 -0.8 111,626 - GFF5736 0.89 +0.6 111,626 - GFF5736 0.89 -0.5 111,626 - GFF5736 0.89 -0.5 111,749 - -0.3 112,219 - GFF5737 0.34 -0.6 112,219 - GFF5737 0.34 -0.4 113,403 + GFF5738 0.55 +1.1 113,442 + GFF5738 0.60 +0.2 113,443 - GFF5738 0.60 -0.2 113,798 + GFF5739 0.16 -1.0 113,799 - GFF5739 0.16 -0.1
Or see this region's nucleotide sequence