Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF570 and GFF571 are separated by 7 nucleotides GFF571 and GFF572 overlap by 4 nucleotides GFF572 and GFF573 overlap by 4 nucleotides
GFF570 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 616,372 to 617,526
GFF570
GFF571 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 617,534 to 617,998
GFF571
GFF572 - Transcription termination protein NusB, at 617,995 to 618,552
GFF572
GFF573 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 618,549 to 619,745
GFF573
Position (kb) 617
618 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 617.395 kb on + strand, within GFF570 at 617.395 kb on + strand, within GFF570 at 617.396 kb on - strand, within GFF570 at 617.488 kb on + strand at 617.489 kb on - strand at 617.509 kb on + strand at 617.509 kb on + strand at 617.509 kb on + strand at 617.510 kb on - strand at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.804 kb on + strand, within GFF571 at 617.864 kb on + strand, within GFF571 at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.588 kb on + strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.589 kb on - strand at 618.727 kb on + strand, within GFF573 at 618.727 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.747 kb on + strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.748 kb on - strand, within GFF573 at 618.798 kb on + strand, within GFF573 at 618.798 kb on + strand, within GFF573 at 618.798 kb on + strand, within GFF573 at 618.930 kb on + strand, within GFF573 at 618.930 kb on + strand, within GFF573 at 618.930 kb on + strand, within GFF573 at 618.931 kb on - strand, within GFF573 at 618.931 kb on - strand, within GFF573 at 618.931 kb on - strand, within GFF573
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 617,395 + GFF570 0.89 -0.7 617,395 + GFF570 0.89 +0.5 617,396 - GFF570 0.89 -0.4 617,488 + -1.2 617,489 - +0.6 617,509 + -1.5 617,509 + +0.5 617,509 + -0.5 617,510 - +0.7 617,804 + GFF571 0.58 -0.5 617,804 + GFF571 0.58 -1.0 617,804 + GFF571 0.58 -0.4 617,804 + GFF571 0.58 -1.3 617,804 + GFF571 0.58 -1.1 617,804 + GFF571 0.58 -1.1 617,804 + GFF571 0.58 -0.5 617,864 + GFF571 0.71 -0.3 618,588 + -0.5 618,588 + -2.5 618,588 + -1.7 618,588 + +1.7 618,588 + -0.2 618,588 + +0.8 618,588 + +0.3 618,588 + +1.2 618,588 + -0.4 618,588 + -1.2 618,588 + +1.2 618,588 + -0.6 618,588 + -0.0 618,588 + -1.2 618,588 + +0.5 618,588 + +0.2 618,588 + -0.6 618,588 + -0.8 618,588 + +0.4 618,588 + +1.2 618,588 + -0.4 618,588 + +0.2 618,589 - -0.2 618,589 - +0.3 618,589 - -1.4 618,589 - -1.8 618,589 - -1.8 618,589 - -0.7 618,589 - -0.7 618,589 - -1.1 618,589 - +0.3 618,589 - +0.4 618,589 - -0.0 618,589 - -0.1 618,589 - -1.0 618,589 - -0.4 618,589 - +0.7 618,589 - -0.7 618,589 - +1.6 618,589 - +0.4 618,589 - +3.0 618,589 - -0.6 618,589 - -1.4 618,589 - -0.6 618,589 - +0.3 618,589 - -0.4 618,589 - +0.3 618,589 - +0.5 618,589 - +1.4 618,589 - +0.5 618,589 - -0.2 618,589 - -0.4 618,589 - -0.2 618,589 - +0.7 618,727 + GFF573 0.15 -0.1 618,727 + GFF573 0.15 +0.1 618,747 + GFF573 0.17 -0.6 618,747 + GFF573 0.17 +1.2 618,747 + GFF573 0.17 -0.1 618,747 + GFF573 0.17 +1.0 618,747 + GFF573 0.17 -0.6 618,747 + GFF573 0.17 +1.2 618,747 + GFF573 0.17 +0.8 618,747 + GFF573 0.17 -1.6 618,747 + GFF573 0.17 -0.4 618,747 + GFF573 0.17 -0.9 618,747 + GFF573 0.17 -0.0 618,747 + GFF573 0.17 -1.1 618,748 - GFF573 0.17 -1.0 618,748 - GFF573 0.17 -0.4 618,748 - GFF573 0.17 +0.9 618,748 - GFF573 0.17 -1.8 618,748 - GFF573 0.17 -0.7 618,748 - GFF573 0.17 +0.3 618,748 - GFF573 0.17 +0.0 618,798 + GFF573 0.21 -0.3 618,798 + GFF573 0.21 -0.1 618,798 + GFF573 0.21 +1.6 618,930 + GFF573 0.32 -0.6 618,930 + GFF573 0.32 -1.4 618,930 + GFF573 0.32 +3.2 618,931 - GFF573 0.32 -0.3 618,931 - GFF573 0.32 -0.2 618,931 - GFF573 0.32 -0.2
Or see this region's nucleotide sequence