Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF55 and GFF56 overlap by 8 nucleotides GFF56 and GFF57 are separated by 67 nucleotides GFF57 and GFF58 are separated by 93 nucleotides
GFF55 - hypothetical protein, at 69,853 to 70,671
GFF55
GFF56 - hypothetical protein, at 70,664 to 71,593
GFF56
GFF57 - Putative polysaccharide export protein YccZ precursor, at 71,661 to 72,872
GFF57
GFF58 - Tyrosine-protein kinase (EC 2.7.10.2), at 72,966 to 75,230
GFF58
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1 at 71.327 kb on + strand, within GFF56 at 71.327 kb on + strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.412 kb on - strand, within GFF56 at 71.616 kb on + strand at 71.617 kb on - strand at 71.983 kb on - strand, within GFF57 at 71.983 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.141 kb on + strand, within GFF57 at 72.274 kb on - strand, within GFF57 at 72.525 kb on + strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.772 kb on - strand at 72.772 kb on - strand at 73.084 kb on - strand at 73.278 kb on + strand, within GFF58 at 73.278 kb on + strand, within GFF58 at 73.279 kb on - strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.378 kb on - strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.620 kb on + strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 71,327 + GFF56 0.71 +0.6 71,327 + GFF56 0.71 -0.1 71,328 - GFF56 0.71 -0.8 71,328 - GFF56 0.71 -0.7 71,328 - GFF56 0.71 -0.5 71,412 - GFF56 0.80 +0.4 71,616 + -0.6 71,617 - -0.7 71,983 - GFF57 0.27 +0.1 71,983 - GFF57 0.27 +0.5 72,022 - GFF57 0.30 -1.4 72,022 - GFF57 0.30 +0.2 72,022 - GFF57 0.30 +0.0 72,141 + GFF57 0.40 -0.0 72,274 - GFF57 0.51 +0.1 72,525 + GFF57 0.71 +0.5 72,526 - GFF57 0.71 +0.0 72,526 - GFF57 0.71 +0.1 72,651 + GFF57 0.82 +0.8 72,651 + GFF57 0.82 -0.5 72,651 + GFF57 0.82 -0.6 72,651 + GFF57 0.82 +0.1 72,652 - GFF57 0.82 +0.5 72,652 - GFF57 0.82 +0.7 72,652 - GFF57 0.82 -0.8 72,652 - GFF57 0.82 -0.3 72,652 - GFF57 0.82 -1.4 72,771 + +0.4 72,771 + -0.7 72,771 + +0.6 72,771 + +0.1 72,772 - +0.7 72,772 - +0.2 73,084 - -0.9 73,278 + GFF58 0.14 +0.2 73,278 + GFF58 0.14 +1.5 73,279 - GFF58 0.14 -1.9 73,299 + GFF58 0.15 -1.1 73,299 + GFF58 0.15 -0.3 73,299 + GFF58 0.15 -0.3 73,299 + GFF58 0.15 -0.9 73,299 + GFF58 0.15 -1.8 73,299 + GFF58 0.15 -0.1 73,300 - GFF58 0.15 +0.3 73,300 - GFF58 0.15 +1.0 73,300 - GFF58 0.15 -0.7 73,300 - GFF58 0.15 -0.6 73,300 - GFF58 0.15 -0.4 73,378 - GFF58 0.18 +0.1 73,476 + GFF58 0.23 -1.6 73,476 + GFF58 0.23 -0.2 73,477 - GFF58 0.23 -0.3 73,477 - GFF58 0.23 -0.9 73,620 + GFF58 0.29 -1.1 73,621 - GFF58 0.29 +1.7 73,621 - GFF58 0.29 -0.0 73,764 + GFF58 0.35 -0.8 73,764 + GFF58 0.35 -1.0 73,764 + GFF58 0.35 +0.1 73,765 - GFF58 0.35 -0.0 73,765 - GFF58 0.35 -2.2 73,765 - GFF58 0.35 -1.4
Or see this region's nucleotide sequence