Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5685 and GFF5686 are separated by 25 nucleotides GFF5686 and GFF5687 overlap by 8 nucleotides GFF5687 and GFF5688 are separated by 115 nucleotides
GFF5685 - Gluconate 5-dehydrogenase (EC 1.1.1.69), at 61,529 to 62,335
GFF5685
GFF5686 - FIG01027227: hypothetical protein, at 62,361 to 62,759
GFF5686
GFF5687 - FIG01027591: hypothetical protein, at 62,752 to 63,306
GFF5687
GFF5688 - Transcriptional regulator, LysR family, at 63,422 to 64,345
GFF5688
Position (kb) 62
63
64 Strain fitness (log2 ratio) -2
-1
0
1
2 at 61.830 kb on + strand, within GFF5685 at 61.830 kb on + strand, within GFF5685 at 61.831 kb on - strand, within GFF5685 at 61.872 kb on + strand, within GFF5685 at 61.872 kb on + strand, within GFF5685 at 62.449 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.710 kb on + strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.711 kb on - strand, within GFF5686 at 62.810 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.032 kb on + strand, within GFF5687 at 63.033 kb on - strand, within GFF5687 at 63.033 kb on - strand, within GFF5687 at 63.359 kb on + strand at 63.359 kb on + strand at 63.359 kb on + strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.360 kb on - strand at 63.534 kb on - strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.728 kb on + strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688 at 63.729 kb on - strand, within GFF5688
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 61,830 + GFF5685 0.37 -1.0 61,830 + GFF5685 0.37 +1.0 61,831 - GFF5685 0.37 -0.3 61,872 + GFF5685 0.43 -0.2 61,872 + GFF5685 0.43 -0.8 62,449 + GFF5686 0.22 +0.2 62,710 + GFF5686 0.87 +0.2 62,710 + GFF5686 0.87 -0.6 62,711 - GFF5686 0.88 +0.6 62,711 - GFF5686 0.88 +0.0 62,711 - GFF5686 0.88 +0.8 62,810 + GFF5687 0.10 -1.0 63,032 + GFF5687 0.50 -0.4 63,032 + GFF5687 0.50 -0.6 63,032 + GFF5687 0.50 +0.6 63,032 + GFF5687 0.50 -0.0 63,033 - GFF5687 0.51 +0.6 63,033 - GFF5687 0.51 +1.4 63,359 + +0.3 63,359 + -0.4 63,359 + +0.2 63,360 - +0.2 63,360 - +0.7 63,360 - +2.0 63,360 - +0.4 63,360 - +0.2 63,534 - GFF5688 0.12 -2.3 63,728 + GFF5688 0.33 +0.4 63,728 + GFF5688 0.33 -1.0 63,728 + GFF5688 0.33 -0.2 63,729 - GFF5688 0.33 +1.2 63,729 - GFF5688 0.33 +0.6 63,729 - GFF5688 0.33 -0.8 63,729 - GFF5688 0.33 +0.6
Or see this region's nucleotide sequence