Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5374 and GFF5375 are separated by 21 nucleotides GFF5375 and GFF5376 are separated by 31 nucleotides GFF5376 and GFF5377 are separated by 71 nucleotides
GFF5374 - Glutamine amidotransferase, class I, at 539 to 1,483
GFF5374
GFF5375 - Acyl-CoA dehydrogenase, at 1,505 to 3,145
GFF5375
GFF5376 - hypothetical protein, at 3,177 to 4,010
GFF5376
GFF5377 - Transcriptional regulator, AcrR family, at 4,082 to 4,717
GFF5377
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.800 kb on + strand, within GFF5374 at 0.800 kb on + strand, within GFF5374 at 0.801 kb on - strand, within GFF5374 at 0.801 kb on - strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.914 kb on + strand, within GFF5374 at 0.914 kb on + strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.017 kb on - strand, within GFF5374 at 1.017 kb on - strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.926 kb on + strand, within GFF5375 at 1.927 kb on - strand, within GFF5375 at 1.927 kb on - strand, within GFF5375 at 1.927 kb on - strand, within GFF5375 at 1.927 kb on - strand, within GFF5375 at 1.927 kb on - strand, within GFF5375 at 2.178 kb on + strand, within GFF5375 at 2.178 kb on + strand, within GFF5375 at 2.178 kb on + strand, within GFF5375 at 2.178 kb on + strand, within GFF5375 at 2.178 kb on + strand, within GFF5375 at 2.179 kb on - strand, within GFF5375 at 2.179 kb on - strand, within GFF5375 at 2.694 kb on + strand, within GFF5375 at 2.694 kb on + strand, within GFF5375 at 2.694 kb on + strand, within GFF5375 at 2.695 kb on - strand, within GFF5375 at 2.695 kb on - strand, within GFF5375 at 2.695 kb on - strand, within GFF5375 at 2.695 kb on - strand, within GFF5375 at 2.695 kb on - strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.784 kb on + strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.785 kb on - strand, within GFF5375 at 2.800 kb on - strand, within GFF5375 at 2.800 kb on - strand, within GFF5375 at 2.997 kb on + strand at 2.997 kb on + strand at 2.998 kb on - strand at 3.084 kb on + strand at 3.084 kb on + strand at 3.084 kb on + strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.093 kb on + strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.094 kb on - strand at 3.245 kb on - strand at 3.245 kb on - strand at 3.547 kb on + strand, within GFF5376 at 3.547 kb on + strand, within GFF5376 at 3.548 kb on - strand, within GFF5376 at 3.548 kb on - strand, within GFF5376 at 3.548 kb on - strand, within GFF5376 at 3.639 kb on + strand, within GFF5376 at 3.640 kb on - strand, within GFF5376 at 3.640 kb on - strand, within GFF5376 at 3.964 kb on + strand at 3.964 kb on + strand at 3.964 kb on + strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 4.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 692 + GFF5374 0.16 -0.2 692 + GFF5374 0.16 -1.1 692 + GFF5374 0.16 -0.1 692 + GFF5374 0.16 -1.4 692 + GFF5374 0.16 -0.2 692 + GFF5374 0.16 -0.4 692 + GFF5374 0.16 +1.0 692 + GFF5374 0.16 +0.7 692 + GFF5374 0.16 +0.0 693 - GFF5374 0.16 +0.5 693 - GFF5374 0.16 +0.1 693 - GFF5374 0.16 -1.0 693 - GFF5374 0.16 +0.1 800 + GFF5374 0.28 -1.2 800 + GFF5374 0.28 +0.0 801 - GFF5374 0.28 +0.8 801 - GFF5374 0.28 +0.8 860 + GFF5374 0.34 -1.4 860 + GFF5374 0.34 -0.6 860 + GFF5374 0.34 +0.2 860 + GFF5374 0.34 +0.8 861 - GFF5374 0.34 -0.1 861 - GFF5374 0.34 +0.8 861 - GFF5374 0.34 -1.5 861 - GFF5374 0.34 -0.7 861 - GFF5374 0.34 +0.8 861 - GFF5374 0.34 -0.4 861 - GFF5374 0.34 +0.2 914 + GFF5374 0.40 -1.8 914 + GFF5374 0.40 -0.4 915 - GFF5374 0.40 -1.0 915 - GFF5374 0.40 -0.7 915 - GFF5374 0.40 -0.3 915 - GFF5374 0.40 +0.2 915 - GFF5374 0.40 +0.2 1,016 + GFF5374 0.50 +0.6 1,016 + GFF5374 0.50 -2.1 1,016 + GFF5374 0.50 -0.3 1,016 + GFF5374 0.50 +0.2 1,016 + GFF5374 0.50 -0.4 1,016 + GFF5374 0.50 +0.6 1,017 - GFF5374 0.51 -0.4 1,017 - GFF5374 0.51 -0.1 1,079 + GFF5374 0.57 +0.2 1,079 + GFF5374 0.57 +0.8 1,079 + GFF5374 0.57 +0.5 1,079 + GFF5374 0.57 -0.9 1,079 + GFF5374 0.57 -0.3 1,079 + GFF5374 0.57 +0.2 1,079 + GFF5374 0.57 +0.2 1,079 + GFF5374 0.57 -0.4 1,080 - GFF5374 0.57 -1.7 1,080 - GFF5374 0.57 -0.7 1,080 - GFF5374 0.57 -0.1 1,080 - GFF5374 0.57 -0.6 1,080 - GFF5374 0.57 -0.3 1,080 - GFF5374 0.57 -0.8 1,137 - GFF5374 0.63 -0.2 1,137 - GFF5374 0.63 -0.3 1,137 - GFF5374 0.63 -1.5 1,137 - GFF5374 0.63 -1.5 1,280 + GFF5374 0.78 -0.5 1,280 + GFF5374 0.78 -1.1 1,280 + GFF5374 0.78 +0.2 1,280 + GFF5374 0.78 -0.0 1,280 + GFF5374 0.78 -0.1 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 +1.3 1,280 + GFF5374 0.78 -0.4 1,280 + GFF5374 0.78 +1.0 1,280 + GFF5374 0.78 +1.2 1,281 - GFF5374 0.79 -0.0 1,281 - GFF5374 0.79 +0.6 1,281 - GFF5374 0.79 +0.2 1,281 - GFF5374 0.79 -1.3 1,281 - GFF5374 0.79 +1.1 1,319 + GFF5374 0.83 -0.9 1,319 + GFF5374 0.83 -0.5 1,319 + GFF5374 0.83 +0.5 1,319 + GFF5374 0.83 -0.8 1,319 + GFF5374 0.83 -0.2 1,320 - GFF5374 0.83 +0.5 1,320 - GFF5374 0.83 -0.9 1,320 - GFF5374 0.83 -0.6 1,320 - GFF5374 0.83 -0.4 1,320 - GFF5374 0.83 +0.0 1,926 + GFF5375 0.26 +0.1 1,926 + GFF5375 0.26 +0.3 1,926 + GFF5375 0.26 -0.2 1,926 + GFF5375 0.26 +1.5 1,926 + GFF5375 0.26 -1.2 1,926 + GFF5375 0.26 -2.1 1,926 + GFF5375 0.26 -0.5 1,927 - GFF5375 0.26 +0.5 1,927 - GFF5375 0.26 +0.6 1,927 - GFF5375 0.26 +1.0 1,927 - GFF5375 0.26 +0.4 1,927 - GFF5375 0.26 -0.5 2,178 + GFF5375 0.41 +1.4 2,178 + GFF5375 0.41 -0.1 2,178 + GFF5375 0.41 +0.3 2,178 + GFF5375 0.41 +0.1 2,178 + GFF5375 0.41 -0.4 2,179 - GFF5375 0.41 +0.3 2,179 - GFF5375 0.41 -0.2 2,694 + GFF5375 0.72 -0.0 2,694 + GFF5375 0.72 +0.1 2,694 + GFF5375 0.72 +0.9 2,695 - GFF5375 0.73 -0.5 2,695 - GFF5375 0.73 +2.8 2,695 - GFF5375 0.73 -0.1 2,695 - GFF5375 0.73 +0.3 2,695 - GFF5375 0.73 -1.0 2,784 + GFF5375 0.78 -2.6 2,784 + GFF5375 0.78 -0.5 2,784 + GFF5375 0.78 +0.3 2,784 + GFF5375 0.78 -0.5 2,784 + GFF5375 0.78 -0.3 2,784 + GFF5375 0.78 +1.2 2,785 - GFF5375 0.78 +0.3 2,785 - GFF5375 0.78 +0.2 2,785 - GFF5375 0.78 +0.6 2,785 - GFF5375 0.78 +0.7 2,785 - GFF5375 0.78 -0.5 2,785 - GFF5375 0.78 -0.1 2,785 - GFF5375 0.78 +0.0 2,785 - GFF5375 0.78 -1.0 2,785 - GFF5375 0.78 -0.2 2,785 - GFF5375 0.78 -2.5 2,785 - GFF5375 0.78 +1.0 2,785 - GFF5375 0.78 +0.9 2,800 - GFF5375 0.79 -1.3 2,800 - GFF5375 0.79 +1.0 2,997 + +0.0 2,997 + -0.1 2,998 - -0.8 3,084 + -1.3 3,084 + +0.1 3,084 + -0.4 3,085 - +0.1 3,085 - -0.2 3,085 - -0.6 3,085 - -0.8 3,085 - -0.5 3,085 - -1.0 3,085 - +0.4 3,085 - -0.5 3,085 - -0.1 3,085 - +0.0 3,093 + +0.3 3,093 + +0.5 3,093 + +1.0 3,093 + +0.4 3,093 + +2.2 3,093 + +0.5 3,093 + -0.4 3,093 + -0.4 3,093 + -0.7 3,093 + -0.9 3,094 - -0.8 3,094 - +0.3 3,094 - -1.1 3,094 - -0.2 3,094 - -0.5 3,094 - -1.4 3,094 - +0.6 3,094 - +0.4 3,094 - -2.7 3,245 - +0.1 3,245 - -0.6 3,547 + GFF5376 0.44 +0.7 3,547 + GFF5376 0.44 +1.1 3,548 - GFF5376 0.44 -2.2 3,548 - GFF5376 0.44 -1.2 3,548 - GFF5376 0.44 -1.0 3,639 + GFF5376 0.55 -0.6 3,640 - GFF5376 0.56 +2.1 3,640 - GFF5376 0.56 -1.1 3,964 + -1.4 3,964 + -0.3 3,964 + -0.6 3,965 - +0.3 3,965 - -0.7 3,965 - -0.3 3,965 - -0.3 3,965 - -0.1 3,965 - +0.6 4,043 - -0.2
Or see this region's nucleotide sequence