Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5040 and GFF5041 are separated by 78 nucleotides GFF5041 and GFF5042 are separated by 119 nucleotides
GFF5040 - 3-hydroxypropionate dehydrogenase DddA, flavin-containing (EC 1.1.1.59), at 140,476 to 142,107
GFF5040
GFF5041 - Malonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.18), at 142,186 to 143,697
GFF5041
GFF5042 - Transcriptional regulator, LysR family, at 143,817 to 144,749
GFF5042
Position (kb) 142
143
144 Strain fitness (log2 ratio) -2
-1
0
1
2 at 141.482 kb on + strand, within GFF5040 at 141.482 kb on + strand, within GFF5040 at 141.483 kb on - strand, within GFF5040 at 141.483 kb on - strand, within GFF5040 at 141.483 kb on - strand, within GFF5040 at 141.483 kb on - strand, within GFF5040 at 141.551 kb on + strand, within GFF5040 at 141.551 kb on + strand, within GFF5040 at 141.552 kb on - strand, within GFF5040 at 141.711 kb on - strand, within GFF5040 at 141.711 kb on - strand, within GFF5040 at 141.795 kb on - strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.818 kb on + strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.819 kb on - strand, within GFF5040 at 141.869 kb on + strand, within GFF5040 at 141.914 kb on + strand, within GFF5040 at 141.914 kb on + strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.915 kb on - strand, within GFF5040 at 141.947 kb on + strand at 141.947 kb on + strand at 141.947 kb on + strand at 141.947 kb on + strand at 141.947 kb on + strand at 141.947 kb on + strand at 141.947 kb on + strand at 141.948 kb on - strand at 141.948 kb on - strand at 141.948 kb on - strand at 141.953 kb on + strand at 141.953 kb on + strand at 141.954 kb on - strand at 141.954 kb on - strand at 141.980 kb on + strand at 141.980 kb on + strand at 141.980 kb on + strand at 141.980 kb on + strand at 141.981 kb on - strand at 141.981 kb on - strand at 142.086 kb on - strand at 142.086 kb on - strand at 142.086 kb on - strand at 142.086 kb on - strand at 142.086 kb on - strand at 142.086 kb on - strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.187 kb on + strand at 142.188 kb on - strand at 142.188 kb on - strand at 142.188 kb on - strand at 142.268 kb on + strand at 142.268 kb on + strand at 142.268 kb on + strand at 142.268 kb on + strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.292 kb on + strand at 142.544 kb on + strand, within GFF5041 at 142.544 kb on + strand, within GFF5041 at 142.544 kb on + strand, within GFF5041 at 142.544 kb on + strand, within GFF5041 at 142.544 kb on + strand, within GFF5041 at 142.544 kb on + strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.545 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.689 kb on - strand, within GFF5041 at 142.992 kb on - strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 143.746 kb on + strand at 143.746 kb on + strand at 143.769 kb on + strand at 143.770 kb on - strand at 143.770 kb on - strand at 143.788 kb on + strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.832 kb on - strand at 144.345 kb on + strand, within GFF5042 at 144.345 kb on + strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.669 kb on + strand at 144.670 kb on - strand at 144.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 141,482 + GFF5040 0.62 -0.4 141,482 + GFF5040 0.62 +1.1 141,483 - GFF5040 0.62 -1.6 141,483 - GFF5040 0.62 +1.0 141,483 - GFF5040 0.62 -0.4 141,483 - GFF5040 0.62 -0.9 141,551 + GFF5040 0.66 -0.2 141,551 + GFF5040 0.66 -0.2 141,552 - GFF5040 0.66 -2.1 141,711 - GFF5040 0.76 +0.3 141,711 - GFF5040 0.76 -1.6 141,795 - GFF5040 0.81 -0.8 141,818 + GFF5040 0.82 -1.1 141,818 + GFF5040 0.82 -0.1 141,818 + GFF5040 0.82 -1.0 141,818 + GFF5040 0.82 -1.2 141,818 + GFF5040 0.82 -0.7 141,818 + GFF5040 0.82 +0.7 141,818 + GFF5040 0.82 +0.0 141,818 + GFF5040 0.82 -1.4 141,819 - GFF5040 0.82 -0.9 141,819 - GFF5040 0.82 +0.9 141,819 - GFF5040 0.82 -1.1 141,819 - GFF5040 0.82 -1.1 141,819 - GFF5040 0.82 +1.8 141,819 - GFF5040 0.82 -1.1 141,819 - GFF5040 0.82 +1.8 141,869 + GFF5040 0.85 +0.2 141,914 + GFF5040 0.88 -1.8 141,914 + GFF5040 0.88 +0.3 141,915 - GFF5040 0.88 -0.6 141,915 - GFF5040 0.88 +0.8 141,915 - GFF5040 0.88 -0.5 141,915 - GFF5040 0.88 -0.5 141,915 - GFF5040 0.88 -1.0 141,915 - GFF5040 0.88 -0.2 141,915 - GFF5040 0.88 -1.6 141,947 + -1.2 141,947 + -0.3 141,947 + -1.8 141,947 + -0.1 141,947 + -0.7 141,947 + -0.0 141,947 + +1.1 141,948 - +0.1 141,948 - -2.2 141,948 - -1.0 141,953 + -1.6 141,953 + +1.8 141,954 - -0.2 141,954 - -0.3 141,980 + -0.4 141,980 + +0.9 141,980 + +0.2 141,980 + -1.0 141,981 - -1.3 141,981 - -0.8 142,086 - -1.4 142,086 - -2.3 142,086 - -0.2 142,086 - -1.1 142,086 - +0.0 142,086 - -1.3 142,187 + +0.5 142,187 + -0.6 142,187 + -0.1 142,187 + -0.5 142,187 + -0.4 142,187 + -0.1 142,187 + -0.7 142,188 - -1.0 142,188 - -0.3 142,188 - -0.3 142,268 + +0.9 142,268 + +1.3 142,268 + +1.3 142,268 + +0.9 142,269 - +1.0 142,269 - +0.2 142,269 - -0.8 142,269 - +0.7 142,269 - +0.8 142,269 - -0.0 142,292 + +0.8 142,544 + GFF5041 0.24 +0.4 142,544 + GFF5041 0.24 +0.4 142,544 + GFF5041 0.24 +1.0 142,544 + GFF5041 0.24 -0.3 142,544 + GFF5041 0.24 +0.4 142,544 + GFF5041 0.24 -0.2 142,545 - GFF5041 0.24 -0.8 142,545 - GFF5041 0.24 +1.3 142,545 - GFF5041 0.24 +0.5 142,545 - GFF5041 0.24 -0.4 142,545 - GFF5041 0.24 -2.0 142,545 - GFF5041 0.24 +0.2 142,545 - GFF5041 0.24 +0.1 142,545 - GFF5041 0.24 -0.4 142,545 - GFF5041 0.24 -0.8 142,545 - GFF5041 0.24 -0.4 142,545 - GFF5041 0.24 +1.0 142,545 - GFF5041 0.24 -0.1 142,689 - GFF5041 0.33 +0.9 142,689 - GFF5041 0.33 +1.0 142,689 - GFF5041 0.33 +0.4 142,689 - GFF5041 0.33 +0.5 142,689 - GFF5041 0.33 +0.7 142,689 - GFF5041 0.33 +0.8 142,992 - GFF5041 0.53 +0.2 142,997 + GFF5041 0.54 +1.6 142,997 + GFF5041 0.54 +0.9 142,997 + GFF5041 0.54 +0.7 142,997 + GFF5041 0.54 +0.3 142,997 + GFF5041 0.54 +0.6 142,997 + GFF5041 0.54 +0.7 142,997 + GFF5041 0.54 +0.8 142,997 + GFF5041 0.54 +0.9 142,997 + GFF5041 0.54 +0.5 142,997 + GFF5041 0.54 +0.3 142,997 + GFF5041 0.54 +0.6 142,997 + GFF5041 0.54 +0.4 142,997 + GFF5041 0.54 -0.4 142,997 + GFF5041 0.54 +0.8 142,997 + GFF5041 0.54 +1.2 142,997 + GFF5041 0.54 +0.4 142,997 + GFF5041 0.54 +0.1 142,997 + GFF5041 0.54 +2.4 142,998 - GFF5041 0.54 +0.1 142,998 - GFF5041 0.54 -0.2 142,998 - GFF5041 0.54 +2.0 142,998 - GFF5041 0.54 -0.6 142,998 - GFF5041 0.54 -0.5 142,998 - GFF5041 0.54 +0.8 142,998 - GFF5041 0.54 -0.4 142,998 - GFF5041 0.54 +0.3 142,998 - GFF5041 0.54 +0.2 142,998 - GFF5041 0.54 -0.1 142,998 - GFF5041 0.54 +0.6 142,998 - GFF5041 0.54 -0.8 142,998 - GFF5041 0.54 +0.1 142,998 - GFF5041 0.54 +0.9 142,998 - GFF5041 0.54 +1.4 143,746 + -2.3 143,746 + -0.8 143,769 + +0.1 143,770 - +0.6 143,770 - -0.9 143,788 + -1.0 143,789 - -0.7 143,789 - +0.3 143,832 - -1.2 144,345 + GFF5042 0.57 +0.7 144,345 + GFF5042 0.57 +0.4 144,346 - GFF5042 0.57 -0.3 144,346 - GFF5042 0.57 -0.6 144,654 + GFF5042 0.90 -0.1 144,654 + GFF5042 0.90 +0.3 144,654 + GFF5042 0.90 +2.0 144,669 + -1.0 144,670 - +0.2 144,670 - -0.1
Or see this region's nucleotide sequence