Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4998 and GFF4999 are separated by 12 nucleotides GFF4999 and GFF5000 overlap by 8 nucleotides GFF5000 and GFF5001 overlap by 4 nucleotides
GFF4998 - L-carnitine dehydratase/bile acid-inducible protein F, at 96,817 to 98,058
GFF4998
GFF4999 - hypothetical protein, at 98,071 to 98,385
GFF4999
GFF5000 - PUTATIVE ZINC PROTEASE PROTEIN, at 98,378 to 99,502
GFF5000
GFF5001 - Oxidoreductase, molybdopterin-binding, at 99,499 to 101,559
GFF5001
Position (kb)
98
99
100 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 97.409 kb on + strand, within GFF4998 at 97.409 kb on + strand, within GFF4998 at 97.409 kb on + strand, within GFF4998 at 97.410 kb on - strand, within GFF4998 at 97.410 kb on - strand, within GFF4998 at 97.410 kb on - strand, within GFF4998 at 97.634 kb on + strand, within GFF4998 at 97.925 kb on + strand, within GFF4998 at 98.273 kb on + strand, within GFF4999 at 98.273 kb on + strand, within GFF4999 at 98.273 kb on + strand, within GFF4999 at 98.273 kb on + strand, within GFF4999 at 98.274 kb on - strand, within GFF4999 at 98.274 kb on - strand, within GFF4999 at 98.274 kb on - strand, within GFF4999 at 98.274 kb on - strand, within GFF4999 at 98.274 kb on - strand, within GFF4999 at 98.370 kb on + strand at 98.371 kb on - strand at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.505 kb on + strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.506 kb on - strand, within GFF5000 at 98.571 kb on + strand, within GFF5000 at 98.571 kb on + strand, within GFF5000 at 98.571 kb on + strand, within GFF5000 at 98.571 kb on + strand, within GFF5000 at 98.625 kb on + strand, within GFF5000 at 98.626 kb on - strand, within GFF5000 at 98.626 kb on - strand, within GFF5000 at 98.671 kb on - strand, within GFF5000 at 98.671 kb on - strand, within GFF5000 at 98.754 kb on + strand, within GFF5000 at 98.754 kb on + strand, within GFF5000 at 98.754 kb on + strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.755 kb on - strand, within GFF5000 at 98.830 kb on - strand, within GFF5000 at 98.830 kb on - strand, within GFF5000 at 98.904 kb on + strand, within GFF5000 at 98.904 kb on + strand, within GFF5000 at 98.904 kb on + strand, within GFF5000 at 98.904 kb on + strand, within GFF5000 at 98.904 kb on + strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.905 kb on - strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.937 kb on + strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 98.938 kb on - strand, within GFF5000 at 99.057 kb on + strand, within GFF5000 at 99.057 kb on + strand, within GFF5000 at 99.057 kb on + strand, within GFF5000 at 99.057 kb on + strand, within GFF5000 at 99.057 kb on + strand, within GFF5000 at 99.058 kb on - strand, within GFF5000 at 99.058 kb on - strand, within GFF5000 at 99.058 kb on - strand, within GFF5000 at 99.058 kb on - strand, within GFF5000 at 99.073 kb on - strand, within GFF5000 at 99.073 kb on - strand, within GFF5000 at 99.073 kb on - strand, within GFF5000 at 99.138 kb on + strand, within GFF5000 at 99.138 kb on + strand, within GFF5000 at 99.139 kb on - strand, within GFF5000 at 99.139 kb on - strand, within GFF5000 at 99.141 kb on + strand, within GFF5000 at 99.142 kb on - strand, within GFF5000 at 99.142 kb on - strand, within GFF5000 at 99.142 kb on - strand, within GFF5000 at 99.202 kb on - strand, within GFF5000 at 99.255 kb on + strand, within GFF5000 at 99.255 kb on + strand, within GFF5000 at 99.255 kb on + strand, within GFF5000 at 99.255 kb on + strand, within GFF5000 at 99.255 kb on + strand, within GFF5000 at 99.256 kb on - strand, within GFF5000 at 99.256 kb on - strand, within GFF5000 at 99.256 kb on - strand, within GFF5000 at 99.256 kb on - strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.264 kb on + strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.265 kb on - strand, within GFF5000 at 99.432 kb on + strand at 99.432 kb on + strand at 99.432 kb on + strand at 99.432 kb on + strand at 99.432 kb on + strand at 99.432 kb on + strand at 99.432 kb on + strand at 99.433 kb on - strand at 99.433 kb on - strand at 99.433 kb on - strand at 99.433 kb on - strand at 99.433 kb on - strand at 99.433 kb on - strand at 99.435 kb on + strand at 99.435 kb on + strand at 99.436 kb on - strand at 99.436 kb on - strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.530 kb on + strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.531 kb on - strand at 99.693 kb on - strand at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.214 kb on + strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.215 kb on - strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.265 kb on + strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.266 kb on - strand, within GFF5001 at 100.388 kb on + strand, within GFF5001 at 100.388 kb on + strand, within GFF5001 at 100.389 kb on - strand, within GFF5001 at 100.389 kb on - strand, within GFF5001 at 100.389 kb on - strand, within GFF5001 at 100.389 kb on - strand, within GFF5001 at 100.389 kb on - strand, within GFF5001 at 100.447 kb on - strand, within GFF5001
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 97,409 + GFF4998 0.48 -0.5 97,409 + GFF4998 0.48 +0.0 97,409 + GFF4998 0.48 +0.8 97,410 - GFF4998 0.48 -0.4 97,410 - GFF4998 0.48 +0.2 97,410 - GFF4998 0.48 +0.3 97,634 + GFF4998 0.66 -0.5 97,925 + GFF4998 0.89 -0.9 98,273 + GFF4999 0.64 -0.4 98,273 + GFF4999 0.64 -0.6 98,273 + GFF4999 0.64 +0.1 98,273 + GFF4999 0.64 -0.5 98,274 - GFF4999 0.64 +0.2 98,274 - GFF4999 0.64 +1.8 98,274 - GFF4999 0.64 -0.9 98,274 - GFF4999 0.64 -1.0 98,274 - GFF4999 0.64 +1.4 98,370 + +1.2 98,371 - -0.9 98,505 + GFF5000 0.11 -0.3 98,505 + GFF5000 0.11 -0.1 98,505 + GFF5000 0.11 +0.1 98,505 + GFF5000 0.11 +1.0 98,505 + GFF5000 0.11 +0.4 98,505 + GFF5000 0.11 -1.3 98,505 + GFF5000 0.11 +2.5 98,505 + GFF5000 0.11 +1.1 98,506 - GFF5000 0.11 +0.3 98,506 - GFF5000 0.11 +1.4 98,506 - GFF5000 0.11 -0.5 98,506 - GFF5000 0.11 +0.4 98,506 - GFF5000 0.11 +0.5 98,506 - GFF5000 0.11 -1.6 98,571 + GFF5000 0.17 +0.2 98,571 + GFF5000 0.17 -0.5 98,571 + GFF5000 0.17 -1.3 98,571 + GFF5000 0.17 -0.2 98,625 + GFF5000 0.22 -0.6 98,626 - GFF5000 0.22 -0.6 98,626 - GFF5000 0.22 +0.5 98,671 - GFF5000 0.26 +1.4 98,671 - GFF5000 0.26 -0.0 98,754 + GFF5000 0.33 -0.2 98,754 + GFF5000 0.33 +1.4 98,754 + GFF5000 0.33 +0.4 98,755 - GFF5000 0.34 -0.2 98,755 - GFF5000 0.34 -1.0 98,755 - GFF5000 0.34 -0.5 98,755 - GFF5000 0.34 -0.2 98,755 - GFF5000 0.34 -0.0 98,755 - GFF5000 0.34 -0.6 98,755 - GFF5000 0.34 +1.8 98,830 - GFF5000 0.40 +1.1 98,830 - GFF5000 0.40 -0.8 98,904 + GFF5000 0.47 +1.2 98,904 + GFF5000 0.47 +0.2 98,904 + GFF5000 0.47 -0.1 98,904 + GFF5000 0.47 +0.2 98,904 + GFF5000 0.47 +0.9 98,905 - GFF5000 0.47 -0.2 98,905 - GFF5000 0.47 -0.7 98,905 - GFF5000 0.47 -0.5 98,905 - GFF5000 0.47 -0.3 98,905 - GFF5000 0.47 +0.6 98,905 - GFF5000 0.47 +0.6 98,937 + GFF5000 0.50 -0.9 98,937 + GFF5000 0.50 -0.2 98,937 + GFF5000 0.50 -1.0 98,937 + GFF5000 0.50 -1.0 98,937 + GFF5000 0.50 +0.1 98,937 + GFF5000 0.50 -0.1 98,937 + GFF5000 0.50 -0.1 98,937 + GFF5000 0.50 +0.6 98,938 - GFF5000 0.50 +0.3 98,938 - GFF5000 0.50 +0.4 98,938 - GFF5000 0.50 +0.8 98,938 - GFF5000 0.50 -0.4 98,938 - GFF5000 0.50 -0.2 98,938 - GFF5000 0.50 -0.8 98,938 - GFF5000 0.50 +0.4 98,938 - GFF5000 0.50 +0.4 99,057 + GFF5000 0.60 +0.3 99,057 + GFF5000 0.60 -2.0 99,057 + GFF5000 0.60 -0.2 99,057 + GFF5000 0.60 +0.7 99,057 + GFF5000 0.60 -0.4 99,058 - GFF5000 0.60 +1.8 99,058 - GFF5000 0.60 -0.6 99,058 - GFF5000 0.60 +0.4 99,058 - GFF5000 0.60 +1.2 99,073 - GFF5000 0.62 +0.5 99,073 - GFF5000 0.62 -0.8 99,073 - GFF5000 0.62 +0.0 99,138 + GFF5000 0.68 -0.2 99,138 + GFF5000 0.68 -0.2 99,139 - GFF5000 0.68 +0.1 99,139 - GFF5000 0.68 -0.7 99,141 + GFF5000 0.68 -0.0 99,142 - GFF5000 0.68 -1.1 99,142 - GFF5000 0.68 +0.2 99,142 - GFF5000 0.68 -0.2 99,202 - GFF5000 0.73 -0.4 99,255 + GFF5000 0.78 -0.2 99,255 + GFF5000 0.78 -0.6 99,255 + GFF5000 0.78 -0.2 99,255 + GFF5000 0.78 -0.2 99,255 + GFF5000 0.78 -0.0 99,256 - GFF5000 0.78 -0.5 99,256 - GFF5000 0.78 -0.3 99,256 - GFF5000 0.78 +1.4 99,256 - GFF5000 0.78 -0.1 99,264 + GFF5000 0.79 -0.1 99,264 + GFF5000 0.79 +0.1 99,264 + GFF5000 0.79 +0.2 99,264 + GFF5000 0.79 +0.1 99,264 + GFF5000 0.79 -1.8 99,264 + GFF5000 0.79 +1.2 99,264 + GFF5000 0.79 +0.3 99,264 + GFF5000 0.79 +0.9 99,264 + GFF5000 0.79 +0.1 99,264 + GFF5000 0.79 -1.3 99,265 - GFF5000 0.79 +0.4 99,265 - GFF5000 0.79 -1.7 99,265 - GFF5000 0.79 +0.2 99,265 - GFF5000 0.79 +0.4 99,265 - GFF5000 0.79 -1.9 99,265 - GFF5000 0.79 -2.9 99,265 - GFF5000 0.79 +0.3 99,265 - GFF5000 0.79 +0.9 99,265 - GFF5000 0.79 +0.2 99,265 - GFF5000 0.79 +0.8 99,265 - GFF5000 0.79 +2.4 99,265 - GFF5000 0.79 +0.4 99,265 - GFF5000 0.79 -0.4 99,265 - GFF5000 0.79 -0.9 99,265 - GFF5000 0.79 +0.4 99,265 - GFF5000 0.79 +0.2 99,265 - GFF5000 0.79 -0.8 99,265 - GFF5000 0.79 -0.1 99,265 - GFF5000 0.79 -0.4 99,265 - GFF5000 0.79 -0.5 99,265 - GFF5000 0.79 -0.7 99,432 + -0.4 99,432 + -0.5 99,432 + -0.6 99,432 + +0.2 99,432 + -1.2 99,432 + -0.6 99,432 + +0.4 99,433 - +0.1 99,433 - +0.8 99,433 - -0.5 99,433 - -0.6 99,433 - +0.1 99,433 - -0.4 99,435 + -1.7 99,435 + -1.9 99,436 - +0.1 99,436 - -0.8 99,530 + +0.6 99,530 + +0.5 99,530 + +1.4 99,530 + -0.3 99,530 + -0.3 99,530 + +0.4 99,530 + +0.1 99,530 + +0.4 99,530 + -0.1 99,530 + -0.2 99,530 + -0.2 99,531 - -0.6 99,531 - +1.0 99,531 - -0.8 99,531 - -0.4 99,531 - -0.4 99,531 - -0.4 99,531 - +0.5 99,531 - -0.2 99,531 - -0.4 99,531 - -1.4 99,693 - +0.4 100,214 + GFF5001 0.35 -0.7 100,214 + GFF5001 0.35 -0.1 100,214 + GFF5001 0.35 -1.6 100,214 + GFF5001 0.35 +0.6 100,214 + GFF5001 0.35 -1.0 100,214 + GFF5001 0.35 +0.2 100,214 + GFF5001 0.35 +0.5 100,214 + GFF5001 0.35 +0.3 100,214 + GFF5001 0.35 +1.8 100,214 + GFF5001 0.35 +0.1 100,214 + GFF5001 0.35 +0.0 100,214 + GFF5001 0.35 +0.2 100,214 + GFF5001 0.35 -0.0 100,214 + GFF5001 0.35 -0.5 100,215 - GFF5001 0.35 +0.5 100,215 - GFF5001 0.35 -0.9 100,215 - GFF5001 0.35 +0.2 100,215 - GFF5001 0.35 -1.0 100,215 - GFF5001 0.35 +0.8 100,215 - GFF5001 0.35 +0.2 100,215 - GFF5001 0.35 -0.0 100,215 - GFF5001 0.35 +0.1 100,215 - GFF5001 0.35 -0.1 100,265 + GFF5001 0.37 -1.6 100,265 + GFF5001 0.37 -0.6 100,265 + GFF5001 0.37 -0.1 100,265 + GFF5001 0.37 -0.4 100,265 + GFF5001 0.37 -0.9 100,265 + GFF5001 0.37 -0.1 100,266 - GFF5001 0.37 -0.4 100,266 - GFF5001 0.37 +0.4 100,266 - GFF5001 0.37 -0.8 100,266 - GFF5001 0.37 +0.9 100,266 - GFF5001 0.37 +0.6 100,266 - GFF5001 0.37 +0.3 100,266 - GFF5001 0.37 -0.3 100,388 + GFF5001 0.43 -0.6 100,388 + GFF5001 0.43 -0.6 100,389 - GFF5001 0.43 -0.1 100,389 - GFF5001 0.43 -0.8 100,389 - GFF5001 0.43 +1.2 100,389 - GFF5001 0.43 -1.1 100,389 - GFF5001 0.43 +0.2 100,447 - GFF5001 0.46 -0.8
Or see this region's nucleotide sequence