Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF493 and GFF494 overlap by 4 nucleotides GFF494 and GFF495 are separated by 130 nucleotides
GFF493 - RND efflux system, inner membrane transporter, at 533,749 to 536,907
GFF493
GFF494 - RND efflux system, membrane fusion protein, at 536,904 to 538,115
GFF494
GFF495 - Kup system potassium uptake protein, at 538,246 to 540,090
GFF495
Position (kb)
536
537
538
539 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 536.069 kb on + strand, within GFF493 at 536.069 kb on + strand, within GFF493 at 536.070 kb on - strand, within GFF493 at 536.070 kb on - strand, within GFF493 at 536.070 kb on - strand, within GFF493 at 536.309 kb on + strand, within GFF493 at 536.309 kb on + strand, within GFF493 at 536.309 kb on + strand, within GFF493 at 536.310 kb on - strand, within GFF493 at 536.310 kb on - strand, within GFF493 at 536.310 kb on - strand, within GFF493 at 536.411 kb on + strand, within GFF493 at 536.411 kb on + strand, within GFF493 at 536.411 kb on + strand, within GFF493 at 536.412 kb on - strand, within GFF493 at 536.438 kb on + strand, within GFF493 at 536.439 kb on - strand, within GFF493 at 536.439 kb on - strand, within GFF493 at 536.450 kb on + strand, within GFF493 at 536.450 kb on + strand, within GFF493 at 536.450 kb on + strand, within GFF493 at 536.451 kb on - strand, within GFF493 at 536.451 kb on - strand, within GFF493 at 536.451 kb on - strand, within GFF493 at 536.540 kb on + strand, within GFF493 at 536.540 kb on + strand, within GFF493 at 536.541 kb on - strand, within GFF493 at 536.541 kb on - strand, within GFF493 at 536.618 kb on + strand at 536.618 kb on + strand at 536.618 kb on + strand at 536.618 kb on + strand at 536.619 kb on - strand at 536.619 kb on - strand at 536.619 kb on - strand at 536.619 kb on - strand at 536.640 kb on - strand at 536.717 kb on + strand at 536.717 kb on + strand at 536.717 kb on + strand at 536.717 kb on + strand at 536.718 kb on - strand at 536.718 kb on - strand at 536.718 kb on - strand at 536.780 kb on + strand at 536.780 kb on + strand at 536.780 kb on + strand at 536.781 kb on - strand at 536.819 kb on + strand at 536.820 kb on - strand at 536.820 kb on - strand at 536.820 kb on - strand at 537.031 kb on + strand, within GFF494 at 537.031 kb on + strand, within GFF494 at 537.031 kb on + strand, within GFF494 at 537.031 kb on + strand, within GFF494 at 537.031 kb on + strand, within GFF494 at 537.031 kb on + strand, within GFF494 at 537.032 kb on - strand, within GFF494 at 537.032 kb on - strand, within GFF494 at 537.032 kb on - strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.283 kb on + strand, within GFF494 at 537.284 kb on - strand, within GFF494 at 537.284 kb on - strand, within GFF494 at 537.284 kb on - strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.340 kb on + strand, within GFF494 at 537.341 kb on - strand, within GFF494 at 537.341 kb on - strand, within GFF494 at 537.341 kb on - strand, within GFF494 at 537.341 kb on - strand, within GFF494 at 537.341 kb on - strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.598 kb on + strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.599 kb on - strand, within GFF494 at 537.753 kb on + strand, within GFF494 at 537.753 kb on + strand, within GFF494 at 537.754 kb on - strand, within GFF494 at 537.815 kb on - strand, within GFF494 at 537.815 kb on - strand, within GFF494 at 538.165 kb on + strand at 538.175 kb on + strand at 538.175 kb on + strand at 538.186 kb on + strand at 538.186 kb on + strand at 538.186 kb on + strand at 538.186 kb on + strand at 538.186 kb on + strand at 538.187 kb on - strand at 538.187 kb on - strand at 538.210 kb on + strand at 538.247 kb on + strand at 538.247 kb on + strand at 538.247 kb on + strand at 538.247 kb on + strand at 538.247 kb on + strand at 538.248 kb on - strand at 538.248 kb on - strand at 538.248 kb on - strand at 538.248 kb on - strand at 538.248 kb on - strand at 538.295 kb on + strand at 538.295 kb on + strand at 538.295 kb on + strand at 538.296 kb on - strand at 538.296 kb on - strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.403 kb on + strand at 538.404 kb on - strand at 538.404 kb on - strand at 538.404 kb on - strand at 538.404 kb on - strand at 538.442 kb on + strand, within GFF495 at 538.443 kb on - strand, within GFF495 at 538.513 kb on + strand, within GFF495 at 538.513 kb on + strand, within GFF495 at 538.513 kb on + strand, within GFF495 at 538.513 kb on + strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 538.514 kb on - strand, within GFF495 at 539.012 kb on + strand, within GFF495 at 539.012 kb on + strand, within GFF495 at 539.012 kb on + strand, within GFF495 at 539.099 kb on + strand, within GFF495 at 539.099 kb on + strand, within GFF495 at 539.099 kb on + strand, within GFF495 at 539.099 kb on + strand, within GFF495 at 539.099 kb on + strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495 at 539.100 kb on - strand, within GFF495
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 536,069 + GFF493 0.73 -1.0 536,069 + GFF493 0.73 -0.4 536,070 - GFF493 0.73 -0.4 536,070 - GFF493 0.73 -0.8 536,070 - GFF493 0.73 -0.3 536,309 + GFF493 0.81 +0.2 536,309 + GFF493 0.81 -0.7 536,309 + GFF493 0.81 -0.5 536,310 - GFF493 0.81 -0.9 536,310 - GFF493 0.81 -0.4 536,310 - GFF493 0.81 -0.4 536,411 + GFF493 0.84 +0.7 536,411 + GFF493 0.84 +0.6 536,411 + GFF493 0.84 +0.3 536,412 - GFF493 0.84 -1.1 536,438 + GFF493 0.85 +0.5 536,439 - GFF493 0.85 +0.2 536,439 - GFF493 0.85 -0.1 536,450 + GFF493 0.86 +2.1 536,450 + GFF493 0.86 -0.6 536,450 + GFF493 0.86 -0.6 536,451 - GFF493 0.86 -1.1 536,451 - GFF493 0.86 +3.5 536,451 - GFF493 0.86 -1.0 536,540 + GFF493 0.88 -0.1 536,540 + GFF493 0.88 -0.0 536,541 - GFF493 0.88 -1.4 536,541 - GFF493 0.88 +0.1 536,618 + -0.7 536,618 + -0.7 536,618 + -0.4 536,618 + +0.2 536,619 - -1.8 536,619 - +0.3 536,619 - -0.1 536,619 - -1.6 536,640 - +0.5 536,717 + -0.9 536,717 + -0.6 536,717 + -0.1 536,717 + -0.5 536,718 - +0.2 536,718 - -2.0 536,718 - -0.1 536,780 + +0.4 536,780 + -0.3 536,780 + -1.0 536,781 - -0.0 536,819 + +0.1 536,820 - -0.3 536,820 - -2.4 536,820 - -0.0 537,031 + GFF494 0.10 -0.5 537,031 + GFF494 0.10 +0.4 537,031 + GFF494 0.10 +0.2 537,031 + GFF494 0.10 +1.0 537,031 + GFF494 0.10 +0.7 537,031 + GFF494 0.10 +1.2 537,032 - GFF494 0.11 +0.1 537,032 - GFF494 0.11 +0.6 537,032 - GFF494 0.11 +0.3 537,283 + GFF494 0.31 -1.0 537,283 + GFF494 0.31 -0.1 537,283 + GFF494 0.31 -1.1 537,283 + GFF494 0.31 +1.1 537,283 + GFF494 0.31 +0.5 537,283 + GFF494 0.31 +0.2 537,284 - GFF494 0.31 +0.6 537,284 - GFF494 0.31 -0.9 537,284 - GFF494 0.31 -1.3 537,340 + GFF494 0.36 -0.5 537,340 + GFF494 0.36 -1.0 537,340 + GFF494 0.36 -0.4 537,340 + GFF494 0.36 +0.4 537,340 + GFF494 0.36 +1.6 537,340 + GFF494 0.36 +0.4 537,340 + GFF494 0.36 +0.4 537,340 + GFF494 0.36 +0.0 537,340 + GFF494 0.36 -0.5 537,341 - GFF494 0.36 +0.8 537,341 - GFF494 0.36 -2.4 537,341 - GFF494 0.36 +0.6 537,341 - GFF494 0.36 -0.0 537,341 - GFF494 0.36 +0.0 537,598 + GFF494 0.57 +0.3 537,598 + GFF494 0.57 -0.0 537,598 + GFF494 0.57 -0.9 537,598 + GFF494 0.57 -0.7 537,598 + GFF494 0.57 -0.1 537,598 + GFF494 0.57 +0.7 537,599 - GFF494 0.57 -0.3 537,599 - GFF494 0.57 +0.2 537,599 - GFF494 0.57 -1.0 537,599 - GFF494 0.57 -0.8 537,599 - GFF494 0.57 -1.4 537,599 - GFF494 0.57 +0.8 537,599 - GFF494 0.57 +0.0 537,599 - GFF494 0.57 +0.3 537,753 + GFF494 0.70 +0.2 537,753 + GFF494 0.70 -0.1 537,754 - GFF494 0.70 +0.2 537,815 - GFF494 0.75 -1.1 537,815 - GFF494 0.75 -0.9 538,165 + -0.8 538,175 + -0.6 538,175 + -0.2 538,186 + -0.9 538,186 + +0.2 538,186 + +1.2 538,186 + -0.5 538,186 + +0.3 538,187 - -0.5 538,187 - -1.5 538,210 + -0.1 538,247 + -0.6 538,247 + +1.2 538,247 + +0.2 538,247 + -0.2 538,247 + -2.6 538,248 - -0.6 538,248 - +0.2 538,248 - -0.3 538,248 - -0.6 538,248 - -0.3 538,295 + -1.2 538,295 + +0.3 538,295 + -0.0 538,296 - +1.0 538,296 - -0.3 538,403 + -0.7 538,403 + -0.4 538,403 + -1.0 538,403 + -0.4 538,403 + -1.9 538,403 + +0.1 538,403 + +0.2 538,404 - -0.4 538,404 - -1.3 538,404 - +0.7 538,404 - -1.1 538,442 + GFF495 0.11 +0.5 538,443 - GFF495 0.11 -1.3 538,513 + GFF495 0.14 -2.0 538,513 + GFF495 0.14 -2.4 538,513 + GFF495 0.14 -0.2 538,513 + GFF495 0.14 -0.3 538,514 - GFF495 0.15 -0.5 538,514 - GFF495 0.15 -0.5 538,514 - GFF495 0.15 -0.9 538,514 - GFF495 0.15 -0.8 538,514 - GFF495 0.15 +2.2 538,514 - GFF495 0.15 -0.4 538,514 - GFF495 0.15 +0.7 538,514 - GFF495 0.15 -1.2 539,012 + GFF495 0.42 -0.1 539,012 + GFF495 0.42 +0.9 539,012 + GFF495 0.42 +0.2 539,099 + GFF495 0.46 -1.1 539,099 + GFF495 0.46 +0.7 539,099 + GFF495 0.46 +0.0 539,099 + GFF495 0.46 -0.1 539,099 + GFF495 0.46 +1.6 539,100 - GFF495 0.46 -0.3 539,100 - GFF495 0.46 +1.9 539,100 - GFF495 0.46 -0.2 539,100 - GFF495 0.46 -0.1 539,100 - GFF495 0.46 +0.9 539,100 - GFF495 0.46 +0.4 539,100 - GFF495 0.46 +0.1 539,100 - GFF495 0.46 -0.7
Or see this region's nucleotide sequence