Strain Fitness in Variovorax sp. SCN45 around GFF4855

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4853 and GFF4854 are separated by 17 nucleotidesGFF4854 and GFF4855 are separated by 0 nucleotidesGFF4855 and GFF4856 are separated by 24 nucleotides GFF4853 - Transcriptional regulator, LysR family, at 222,791 to 223,705 GFF4853 GFF4854 - Electron transfer flavoprotein, alpha subunit, at 223,723 to 224,655 GFF4854 GFF4855 - Electron transfer flavoprotein, beta subunit, at 224,656 to 225,405 GFF4855 GFF4856 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 225,430 to 227,211 GFF4856 Position (kb) 224 225 226Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 223.676 kb on + strandat 223.677 kb on - strandat 223.677 kb on - strandat 223.678 kb on + strandat 223.679 kb on - strandat 223.679 kb on - strandat 223.679 kb on - strandat 223.679 kb on - strandat 223.679 kb on - strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.710 kb on + strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.711 kb on - strandat 223.778 kb on + strandat 223.892 kb on + strand, within GFF4854at 223.892 kb on + strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 223.893 kb on - strand, within GFF4854at 224.123 kb on + strand, within GFF4854at 224.123 kb on + strand, within GFF4854at 224.123 kb on + strand, within GFF4854at 224.123 kb on + strand, within GFF4854at 224.123 kb on + strand, within GFF4854at 224.124 kb on - strand, within GFF4854at 224.124 kb on - strand, within GFF4854at 224.124 kb on - strand, within GFF4854at 224.124 kb on - strand, within GFF4854at 224.124 kb on - strand, within GFF4854at 224.273 kb on + strand, within GFF4854at 224.273 kb on + strand, within GFF4854at 224.273 kb on + strand, within GFF4854at 224.273 kb on + strand, within GFF4854at 224.274 kb on - strand, within GFF4854at 224.274 kb on - strand, within GFF4854at 224.274 kb on - strand, within GFF4854at 224.403 kb on + strand, within GFF4854at 224.403 kb on + strand, within GFF4854at 224.403 kb on + strand, within GFF4854at 224.404 kb on - strand, within GFF4854at 224.404 kb on - strand, within GFF4854at 224.404 kb on - strand, within GFF4854at 224.404 kb on - strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.405 kb on + strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.406 kb on - strand, within GFF4854at 224.647 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.657 kb on + strandat 224.658 kb on - strandat 224.658 kb on - strandat 224.852 kb on + strand, within GFF4855at 224.852 kb on + strand, within GFF4855at 224.852 kb on + strand, within GFF4855at 224.853 kb on - strand, within GFF4855at 224.853 kb on - strand, within GFF4855at 225.102 kb on + strand, within GFF4855at 225.102 kb on + strand, within GFF4855at 225.103 kb on - strand, within GFF4855at 225.111 kb on + strand, within GFF4855at 225.151 kb on + strand, within GFF4855at 225.151 kb on + strand, within GFF4855at 225.368 kb on + strandat 225.368 kb on + strandat 225.368 kb on + strandat 225.368 kb on + strandat 225.368 kb on + strandat 225.369 kb on - strandat 225.549 kb on + strandat 225.908 kb on + strand, within GFF4856at 225.908 kb on + strand, within GFF4856at 225.908 kb on + strand, within GFF4856at 225.908 kb on + strand, within GFF4856at 225.908 kb on + strand, within GFF4856at 225.909 kb on - strand, within GFF4856at 225.909 kb on - strand, within GFF4856at 225.909 kb on - strand, within GFF4856at 225.909 kb on - strand, within GFF4856at 225.965 kb on + strand, within GFF4856at 225.965 kb on + strand, within GFF4856at 225.965 kb on + strand, within GFF4856at 225.965 kb on + strand, within GFF4856at 225.965 kb on + strand, within GFF4856at 226.023 kb on + strand, within GFF4856at 226.023 kb on + strand, within GFF4856at 226.023 kb on + strand, within GFF4856at 226.024 kb on - strand, within GFF4856at 226.133 kb on + strand, within GFF4856at 226.134 kb on - strand, within GFF4856at 226.134 kb on - strand, within GFF4856at 226.134 kb on - strand, within GFF4856at 226.260 kb on - strand, within GFF4856at 226.260 kb on - strand, within GFF4856at 226.278 kb on - strand, within GFF4856at 226.382 kb on + strand, within GFF4856at 226.382 kb on + strand, within GFF4856

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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223,676 + -1.1
223,677 - +0.3
223,677 - -0.4
223,678 + +1.1
223,679 - -0.0
223,679 - +0.2
223,679 - -0.2
223,679 - -0.0
223,679 - -0.3
223,710 + -0.3
223,710 + +0.0
223,710 + +1.1
223,710 + -0.2
223,710 + -0.8
223,710 + -0.4
223,710 + -0.2
223,710 + -1.1
223,710 + -0.5
223,711 - +1.0
223,711 - -0.1
223,711 - +0.3
223,711 - -0.0
223,711 - -0.5
223,711 - -0.2
223,711 - -0.5
223,711 - +3.6
223,711 - +1.3
223,778 + +0.9
223,892 + GFF4854 0.18 -0.3
223,892 + GFF4854 0.18 +0.9
223,893 - GFF4854 0.18 -0.8
223,893 - GFF4854 0.18 +1.3
223,893 - GFF4854 0.18 +0.1
223,893 - GFF4854 0.18 -1.3
223,893 - GFF4854 0.18 +0.0
223,893 - GFF4854 0.18 -0.3
223,893 - GFF4854 0.18 -0.3
224,123 + GFF4854 0.43 +1.0
224,123 + GFF4854 0.43 +1.3
224,123 + GFF4854 0.43 +1.7
224,123 + GFF4854 0.43 -0.4
224,123 + GFF4854 0.43 -0.0
224,124 - GFF4854 0.43 -0.5
224,124 - GFF4854 0.43 -0.3
224,124 - GFF4854 0.43 +0.0
224,124 - GFF4854 0.43 -0.7
224,124 - GFF4854 0.43 +0.1
224,273 + GFF4854 0.59 +0.1
224,273 + GFF4854 0.59 -1.5
224,273 + GFF4854 0.59 -0.7
224,273 + GFF4854 0.59 -0.1
224,274 - GFF4854 0.59 +0.1
224,274 - GFF4854 0.59 +0.2
224,274 - GFF4854 0.59 -0.4
224,403 + GFF4854 0.73 +0.5
224,403 + GFF4854 0.73 -1.4
224,403 + GFF4854 0.73 +0.6
224,404 - GFF4854 0.73 +0.9
224,404 - GFF4854 0.73 +1.2
224,404 - GFF4854 0.73 -1.4
224,404 - GFF4854 0.73 +0.4
224,405 + GFF4854 0.73 +0.1
224,405 + GFF4854 0.73 -1.5
224,405 + GFF4854 0.73 +1.3
224,405 + GFF4854 0.73 -1.4
224,405 + GFF4854 0.73 -0.5
224,405 + GFF4854 0.73 +0.7
224,405 + GFF4854 0.73 +1.0
224,405 + GFF4854 0.73 -0.5
224,405 + GFF4854 0.73 -0.5
224,405 + GFF4854 0.73 +0.3
224,405 + GFF4854 0.73 +0.0
224,405 + GFF4854 0.73 +0.4
224,405 + GFF4854 0.73 -0.9
224,405 + GFF4854 0.73 +1.0
224,405 + GFF4854 0.73 -0.2
224,405 + GFF4854 0.73 -0.7
224,405 + GFF4854 0.73 +0.3
224,405 + GFF4854 0.73 +0.6
224,405 + GFF4854 0.73 -0.5
224,406 - GFF4854 0.73 +0.7
224,406 - GFF4854 0.73 -1.0
224,406 - GFF4854 0.73 -0.0
224,406 - GFF4854 0.73 -0.9
224,406 - GFF4854 0.73 +1.3
224,406 - GFF4854 0.73 -0.3
224,406 - GFF4854 0.73 +0.1
224,406 - GFF4854 0.73 -2.4
224,406 - GFF4854 0.73 -0.2
224,406 - GFF4854 0.73 +0.4
224,406 - GFF4854 0.73 -0.4
224,647 + -0.1
224,657 + -0.6
224,657 + -1.0
224,657 + -0.9
224,657 + -0.7
224,657 + -0.6
224,657 + -0.3
224,657 + -0.3
224,658 - -0.6
224,658 - +0.3
224,852 + GFF4855 0.26 -3.1
224,852 + GFF4855 0.26 +0.2
224,852 + GFF4855 0.26 +1.4
224,853 - GFF4855 0.26 +0.2
224,853 - GFF4855 0.26 -1.5
225,102 + GFF4855 0.59 +0.3
225,102 + GFF4855 0.59 +1.9
225,103 - GFF4855 0.60 -0.6
225,111 + GFF4855 0.61 +1.3
225,151 + GFF4855 0.66 +0.3
225,151 + GFF4855 0.66 +0.7
225,368 + -1.1
225,368 + +0.6
225,368 + -0.4
225,368 + +0.1
225,368 + +0.2
225,369 - +0.6
225,549 + +0.0
225,908 + GFF4856 0.27 +0.3
225,908 + GFF4856 0.27 -0.7
225,908 + GFF4856 0.27 -0.3
225,908 + GFF4856 0.27 -0.3
225,908 + GFF4856 0.27 +0.3
225,909 - GFF4856 0.27 +1.8
225,909 - GFF4856 0.27 -0.6
225,909 - GFF4856 0.27 -0.3
225,909 - GFF4856 0.27 +0.3
225,965 + GFF4856 0.30 -0.3
225,965 + GFF4856 0.30 -0.5
225,965 + GFF4856 0.30 +0.8
225,965 + GFF4856 0.30 +0.2
225,965 + GFF4856 0.30 +0.6
226,023 + GFF4856 0.33 -0.6
226,023 + GFF4856 0.33 -0.9
226,023 + GFF4856 0.33 +0.0
226,024 - GFF4856 0.33 +0.4
226,133 + GFF4856 0.39 +0.3
226,134 - GFF4856 0.40 +0.7
226,134 - GFF4856 0.40 -0.4
226,134 - GFF4856 0.40 +0.1
226,260 - GFF4856 0.47 -0.7
226,260 - GFF4856 0.47 -0.1
226,278 - GFF4856 0.48 +0.3
226,382 + GFF4856 0.53 -0.5
226,382 + GFF4856 0.53 +0.5

Or see this region's nucleotide sequence