Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4777 and GFF4778 overlap by 4 nucleotides GFF4778 and GFF4779 are separated by 10 nucleotides GFF4779 and GFF4780 are separated by 76 nucleotides GFF4780 and GFF4781 are separated by 93 nucleotides
GFF4777 - no description, at 145,743 to 145,901
GFF4777
GFF4778 - Broad-specificity amino acid ABC transporter, permease protein 1, at 145,898 to 146,773
GFF4778
GFF4779 - Broad-specificity amino acid ABC transporter, permease protein 2, at 146,784 to 147,776
GFF4779
GFF4780 - hypothetical protein, at 147,853 to 148,308
GFF4780
GFF4781 - AttH component of AttEFGH ABC transport system, at 148,402 to 149,469
GFF4781
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 145.891 kb on + strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.897 kb on + strand at 145.898 kb on - strand at 145.898 kb on - strand at 145.903 kb on + strand at 145.904 kb on - strand at 145.961 kb on + strand at 145.961 kb on + strand at 145.961 kb on + strand at 145.962 kb on - strand at 146.090 kb on + strand, within GFF4778 at 146.090 kb on + strand, within GFF4778 at 146.091 kb on - strand, within GFF4778 at 146.171 kb on + strand, within GFF4778 at 146.172 kb on - strand, within GFF4778 at 146.249 kb on + strand, within GFF4778 at 146.249 kb on + strand, within GFF4778 at 146.250 kb on - strand, within GFF4778 at 146.330 kb on + strand, within GFF4778 at 146.381 kb on + strand, within GFF4778 at 146.381 kb on + strand, within GFF4778 at 146.382 kb on - strand, within GFF4778 at 146.411 kb on + strand, within GFF4778 at 146.411 kb on + strand, within GFF4778 at 146.412 kb on - strand, within GFF4778 at 146.489 kb on + strand, within GFF4778 at 146.490 kb on - strand, within GFF4778 at 146.771 kb on + strand at 146.800 kb on + strand at 146.982 kb on + strand, within GFF4779 at 146.982 kb on + strand, within GFF4779 at 147.108 kb on + strand, within GFF4779 at 147.108 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.256 kb on - strand, within GFF4779 at 147.256 kb on - strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.774 kb on + strand at 147.775 kb on - strand at 147.775 kb on - strand at 147.775 kb on - strand at 148.053 kb on - strand, within GFF4780 at 148.053 kb on - strand, within GFF4780 at 148.058 kb on + strand, within GFF4780 at 148.058 kb on + strand, within GFF4780 at 148.059 kb on - strand, within GFF4780 at 148.100 kb on + strand, within GFF4780 at 148.100 kb on + strand, within GFF4780 at 148.100 kb on + strand, within GFF4780 at 148.100 kb on + strand, within GFF4780 at 148.101 kb on - strand, within GFF4780 at 148.308 kb on + strand at 148.343 kb on + strand at 148.386 kb on + strand at 148.386 kb on + strand at 148.387 kb on - strand at 148.387 kb on - strand at 148.387 kb on - strand at 148.394 kb on + strand at 148.394 kb on + strand at 148.394 kb on + strand at 148.395 kb on - strand at 148.395 kb on - strand at 148.395 kb on - strand at 148.403 kb on + strand at 148.403 kb on + strand at 148.403 kb on + strand at 148.404 kb on - strand at 148.404 kb on - strand at 148.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 145,891 + +1.2 145,892 - -0.8 145,892 - +0.6 145,892 - -1.4 145,892 - +0.4 145,897 + -0.8 145,898 - -0.4 145,898 - +0.1 145,903 + -0.8 145,904 - +0.3 145,961 + -0.4 145,961 + -1.1 145,961 + +0.2 145,962 - -1.0 146,090 + GFF4778 0.22 -0.5 146,090 + GFF4778 0.22 +0.1 146,091 - GFF4778 0.22 +0.2 146,171 + GFF4778 0.31 -0.2 146,172 - GFF4778 0.31 +0.3 146,249 + GFF4778 0.40 +0.1 146,249 + GFF4778 0.40 +0.3 146,250 - GFF4778 0.40 -0.1 146,330 + GFF4778 0.49 +1.3 146,381 + GFF4778 0.55 -0.2 146,381 + GFF4778 0.55 +0.6 146,382 - GFF4778 0.55 +0.6 146,411 + GFF4778 0.59 +0.3 146,411 + GFF4778 0.59 -0.8 146,412 - GFF4778 0.59 -1.3 146,489 + GFF4778 0.67 -1.3 146,490 - GFF4778 0.68 +0.7 146,771 + -0.0 146,800 + +0.0 146,982 + GFF4779 0.20 -1.9 146,982 + GFF4779 0.20 -0.3 147,108 + GFF4779 0.33 -0.4 147,108 + GFF4779 0.33 -0.5 147,255 + GFF4779 0.47 +0.5 147,255 + GFF4779 0.47 -0.6 147,255 + GFF4779 0.47 -0.3 147,255 + GFF4779 0.47 +1.9 147,255 + GFF4779 0.47 -0.4 147,255 + GFF4779 0.47 -1.3 147,256 - GFF4779 0.48 -2.7 147,256 - GFF4779 0.48 -0.4 147,258 + GFF4779 0.48 +0.1 147,258 + GFF4779 0.48 -0.1 147,258 + GFF4779 0.48 -1.7 147,258 + GFF4779 0.48 +0.2 147,258 + GFF4779 0.48 -0.1 147,258 + GFF4779 0.48 -0.9 147,259 - GFF4779 0.48 +2.9 147,259 - GFF4779 0.48 -0.9 147,259 - GFF4779 0.48 -1.1 147,259 - GFF4779 0.48 -0.3 147,774 + -0.6 147,775 - -0.5 147,775 - -0.1 147,775 - -1.1 148,053 - GFF4780 0.44 -0.3 148,053 - GFF4780 0.44 -0.3 148,058 + GFF4780 0.45 +0.0 148,058 + GFF4780 0.45 -0.1 148,059 - GFF4780 0.45 -0.5 148,100 + GFF4780 0.54 -0.5 148,100 + GFF4780 0.54 +0.7 148,100 + GFF4780 0.54 -1.1 148,100 + GFF4780 0.54 -1.6 148,101 - GFF4780 0.54 -1.3 148,308 + -0.7 148,343 + -0.0 148,386 + +0.2 148,386 + -0.1 148,387 - -0.6 148,387 - +0.7 148,387 - +0.3 148,394 + -0.2 148,394 + -1.1 148,394 + +0.1 148,395 - +0.9 148,395 - +0.7 148,395 - -0.5 148,403 + +0.3 148,403 + +0.2 148,403 + -0.7 148,404 - -0.2 148,404 - -0.2 148,404 - -0.4
Or see this region's nucleotide sequence