Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4646 and GFF4647 are separated by 53 nucleotides GFF4647 and GFF4648 overlap by 4 nucleotides GFF4648 and GFF4649 are separated by 132 nucleotides
GFF4646 - Magnesium and cobalt transport protein CorA, at 2,509 to 3,486
GFF4646
GFF4647 - no description, at 3,540 to 4,019
GFF4647
GFF4648 - Inner-membrane proton/drug antiporter (MSF type) of tripartite multidrug efflux system, at 4,016 to 5,548
GFF4648
GFF4649 - Iron-chelator utilization protein, at 5,681 to 6,520
GFF4649
Position (kb) 4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3.064 kb on + strand, within GFF4646 at 3.064 kb on + strand, within GFF4646 at 3.064 kb on + strand, within GFF4646 at 3.065 kb on - strand, within GFF4646 at 3.178 kb on + strand, within GFF4646 at 3.178 kb on + strand, within GFF4646 at 3.179 kb on - strand, within GFF4646 at 3.410 kb on - strand at 3.449 kb on - strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.454 kb on + strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.455 kb on - strand at 3.458 kb on - strand at 3.458 kb on - strand at 3.501 kb on - strand at 3.514 kb on + strand at 3.515 kb on - strand at 3.522 kb on + strand at 3.523 kb on - strand at 3.523 kb on - strand at 3.523 kb on - strand at 3.526 kb on + strand at 3.526 kb on + strand at 3.527 kb on - strand at 3.532 kb on - strand at 3.597 kb on + strand, within GFF4647 at 3.598 kb on - strand, within GFF4647 at 3.598 kb on - strand, within GFF4647 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.187 kb on + strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.188 kb on - strand, within GFF4648 at 4.577 kb on + strand, within GFF4648 at 4.577 kb on + strand, within GFF4648 at 4.578 kb on - strand, within GFF4648 at 4.762 kb on + strand, within GFF4648 at 4.762 kb on + strand, within GFF4648 at 4.763 kb on - strand, within GFF4648 at 4.763 kb on - strand, within GFF4648 at 4.925 kb on + strand, within GFF4648 at 4.926 kb on - strand, within GFF4648 at 4.926 kb on - strand, within GFF4648 at 4.926 kb on - strand, within GFF4648 at 5.057 kb on + strand, within GFF4648 at 5.058 kb on - strand, within GFF4648 at 5.429 kb on + strand at 6.228 kb on + strand, within GFF4649 at 6.228 kb on + strand, within GFF4649 at 6.228 kb on + strand, within GFF4649 at 6.229 kb on - strand, within GFF4649 at 6.229 kb on - strand, within GFF4649 at 6.229 kb on - strand, within GFF4649 at 6.505 kb on + strand at 6.505 kb on + strand at 6.505 kb on + strand at 6.505 kb on + strand at 6.505 kb on + strand at 6.506 kb on - strand at 6.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 3,064 + GFF4646 0.57 -0.7 3,064 + GFF4646 0.57 -0.4 3,064 + GFF4646 0.57 -0.3 3,065 - GFF4646 0.57 +0.6 3,178 + GFF4646 0.68 -0.5 3,178 + GFF4646 0.68 -0.2 3,179 - GFF4646 0.69 -0.5 3,410 - +1.5 3,449 - +0.3 3,454 + -0.2 3,454 + +0.4 3,454 + -0.3 3,454 + -1.6 3,454 + -1.7 3,454 + -0.7 3,454 + -2.5 3,454 + -0.2 3,454 + +1.0 3,454 + -0.2 3,454 + -1.9 3,454 + -0.3 3,454 + +0.1 3,455 - +0.7 3,455 - -0.8 3,455 - +0.2 3,455 - -2.1 3,455 - -0.3 3,455 - +0.0 3,455 - +0.4 3,455 - -0.6 3,455 - +0.7 3,455 - +0.1 3,458 - +0.3 3,458 - +0.3 3,501 - +0.3 3,514 + +0.3 3,515 - +0.8 3,522 + +0.5 3,523 - +0.4 3,523 - -1.5 3,523 - -2.0 3,526 + -1.4 3,526 + +0.1 3,527 - -1.0 3,532 - -0.0 3,597 + GFF4647 0.12 -0.3 3,598 - GFF4647 0.12 -0.1 3,598 - GFF4647 0.12 -0.8 4,187 + GFF4648 0.11 -0.5 4,187 + GFF4648 0.11 -0.5 4,187 + GFF4648 0.11 -1.7 4,187 + GFF4648 0.11 -0.3 4,187 + GFF4648 0.11 +0.1 4,187 + GFF4648 0.11 +0.5 4,187 + GFF4648 0.11 +2.3 4,187 + GFF4648 0.11 -0.0 4,187 + GFF4648 0.11 +0.1 4,188 - GFF4648 0.11 -0.1 4,188 - GFF4648 0.11 +0.1 4,188 - GFF4648 0.11 -0.7 4,188 - GFF4648 0.11 +0.3 4,188 - GFF4648 0.11 +0.3 4,188 - GFF4648 0.11 -0.1 4,577 + GFF4648 0.37 -0.3 4,577 + GFF4648 0.37 -0.2 4,578 - GFF4648 0.37 -0.1 4,762 + GFF4648 0.49 -0.2 4,762 + GFF4648 0.49 +0.7 4,763 - GFF4648 0.49 +0.1 4,763 - GFF4648 0.49 -1.9 4,925 + GFF4648 0.59 +0.4 4,926 - GFF4648 0.59 +0.9 4,926 - GFF4648 0.59 -0.5 4,926 - GFF4648 0.59 +1.3 5,057 + GFF4648 0.68 +0.1 5,058 - GFF4648 0.68 -0.5 5,429 + +0.2 6,228 + GFF4649 0.65 -0.3 6,228 + GFF4649 0.65 -0.2 6,228 + GFF4649 0.65 -0.1 6,229 - GFF4649 0.65 -0.9 6,229 - GFF4649 0.65 +0.3 6,229 - GFF4649 0.65 -0.5 6,505 + -0.1 6,505 + +0.1 6,505 + -0.6 6,505 + -0.0 6,505 + -0.5 6,506 - -0.6 6,506 - -0.4
Or see this region's nucleotide sequence