Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4619 and GFF4620 are separated by 26 nucleotides GFF4620 and GFF4621 are separated by 3 nucleotides
GFF4619 - Altronate dehydratase (EC 4.2.1.7), at 260,520 to 262,058
GFF4619
GFF4620 - Gluconolactonase (EC 3.1.1.17), at 262,085 to 263,017
GFF4620
GFF4621 - Hydroxycarboxylate dehydrogenase (NADP+) HcxB, at 263,021 to 264,076
GFF4621
Position (kb) 262
263
264 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 261.096 kb on + strand, within GFF4619 at 261.096 kb on + strand, within GFF4619 at 261.462 kb on + strand, within GFF4619 at 261.462 kb on + strand, within GFF4619 at 261.463 kb on - strand, within GFF4619 at 261.549 kb on + strand, within GFF4619 at 261.549 kb on + strand, within GFF4619 at 261.550 kb on - strand, within GFF4619 at 261.550 kb on - strand, within GFF4619 at 261.681 kb on + strand, within GFF4619 at 261.681 kb on + strand, within GFF4619 at 261.682 kb on - strand, within GFF4619 at 261.682 kb on - strand, within GFF4619 at 261.682 kb on - strand, within GFF4619 at 261.682 kb on - strand, within GFF4619 at 261.687 kb on + strand, within GFF4619 at 261.687 kb on + strand, within GFF4619 at 261.688 kb on - strand, within GFF4619 at 261.688 kb on - strand, within GFF4619 at 261.720 kb on + strand, within GFF4619 at 261.720 kb on + strand, within GFF4619 at 261.720 kb on + strand, within GFF4619 at 261.720 kb on + strand, within GFF4619 at 261.721 kb on - strand, within GFF4619 at 261.738 kb on + strand, within GFF4619 at 261.816 kb on + strand, within GFF4619 at 261.817 kb on - strand, within GFF4619 at 262.056 kb on + strand at 262.056 kb on + strand at 262.056 kb on + strand at 262.056 kb on + strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.342 kb on + strand, within GFF4620 at 262.343 kb on - strand, within GFF4620 at 262.343 kb on - strand, within GFF4620 at 262.520 kb on + strand, within GFF4620 at 262.539 kb on - strand, within GFF4620 at 262.583 kb on + strand, within GFF4620 at 262.611 kb on + strand, within GFF4620 at 262.611 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.680 kb on - strand, within GFF4620 at 262.680 kb on - strand, within GFF4620 at 262.871 kb on + strand, within GFF4620 at 262.872 kb on - strand, within GFF4620 at 262.872 kb on - strand, within GFF4620 at 262.985 kb on + strand at 262.986 kb on - strand at 262.986 kb on - strand at 262.986 kb on - strand at 263.180 kb on + strand, within GFF4621 at 263.181 kb on - strand, within GFF4621 at 263.183 kb on - strand, within GFF4621 at 263.320 kb on - strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.627 kb on + strand, within GFF4621 at 263.789 kb on - strand, within GFF4621 at 263.849 kb on - strand, within GFF4621
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 261,096 + GFF4619 0.37 -2.0 261,096 + GFF4619 0.37 -1.1 261,462 + GFF4619 0.61 -0.8 261,462 + GFF4619 0.61 +1.7 261,463 - GFF4619 0.61 -0.5 261,549 + GFF4619 0.67 -0.5 261,549 + GFF4619 0.67 +0.8 261,550 - GFF4619 0.67 +0.2 261,550 - GFF4619 0.67 +0.2 261,681 + GFF4619 0.75 -0.5 261,681 + GFF4619 0.75 -0.4 261,682 - GFF4619 0.76 -0.5 261,682 - GFF4619 0.76 +0.3 261,682 - GFF4619 0.76 +0.3 261,682 - GFF4619 0.76 +2.2 261,687 + GFF4619 0.76 -0.1 261,687 + GFF4619 0.76 -0.0 261,688 - GFF4619 0.76 -1.6 261,688 - GFF4619 0.76 -1.0 261,720 + GFF4619 0.78 +0.9 261,720 + GFF4619 0.78 -0.7 261,720 + GFF4619 0.78 -0.7 261,720 + GFF4619 0.78 +0.2 261,721 - GFF4619 0.78 +0.9 261,738 + GFF4619 0.79 +0.1 261,816 + GFF4619 0.84 +0.1 261,817 - GFF4619 0.84 +0.0 262,056 + +0.3 262,056 + +0.7 262,056 + +0.1 262,056 + +0.1 262,057 - +0.3 262,057 - -0.9 262,057 - +0.1 262,057 - -0.5 262,057 - +3.3 262,057 - -0.3 262,057 - -0.7 262,057 - -0.1 262,057 - -0.3 262,057 - -0.9 262,342 + GFF4620 0.28 +0.9 262,343 - GFF4620 0.28 +0.3 262,343 - GFF4620 0.28 -0.3 262,520 + GFF4620 0.47 -0.7 262,539 - GFF4620 0.49 +1.1 262,583 + GFF4620 0.53 +0.1 262,611 + GFF4620 0.56 -0.3 262,611 + GFF4620 0.56 -0.1 262,679 + GFF4620 0.64 +0.1 262,679 + GFF4620 0.64 +0.7 262,679 + GFF4620 0.64 +0.3 262,679 + GFF4620 0.64 +0.5 262,679 + GFF4620 0.64 +0.3 262,680 - GFF4620 0.64 -0.3 262,680 - GFF4620 0.64 +0.2 262,871 + GFF4620 0.84 +0.3 262,872 - GFF4620 0.84 +0.3 262,872 - GFF4620 0.84 -0.3 262,985 + -1.8 262,986 - -0.4 262,986 - -0.5 262,986 - -0.1 263,180 + GFF4621 0.15 -2.0 263,181 - GFF4621 0.15 -0.0 263,183 - GFF4621 0.15 +0.1 263,320 - GFF4621 0.28 +2.1 263,555 + GFF4621 0.51 -0.6 263,555 + GFF4621 0.51 -1.1 263,555 + GFF4621 0.51 -0.7 263,556 - GFF4621 0.51 +1.7 263,556 - GFF4621 0.51 +0.6 263,556 - GFF4621 0.51 +0.5 263,627 + GFF4621 0.57 -1.4 263,789 - GFF4621 0.73 -1.3 263,849 - GFF4621 0.78 +0.8
Or see this region's nucleotide sequence