Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF460 and GFF461 are separated by 15 nucleotides GFF461 and GFF462 overlap by 4 nucleotides GFF462 and GFF463 are separated by 100 nucleotides
GFF460 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 498,601 to 500,139
GFF460
GFF461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 500,155 to 500,955
GFF461
GFF462 - BUG/TctC family periplasmic protein, at 500,952 to 501,938
GFF462
GFF463 - Malonyl-CoA decarboxylase (EC 4.1.1.9), at 502,039 to 503,505
GFF463
Position (kb) 500
501
502 Strain fitness (log2 ratio) -2
-1
0
1
2 at 500.039 kb on + strand at 500.040 kb on - strand at 500.148 kb on + strand at 500.148 kb on + strand at 500.148 kb on + strand at 500.149 kb on - strand at 500.149 kb on - strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.228 kb on + strand at 500.228 kb on + strand at 500.229 kb on - strand at 500.402 kb on + strand, within GFF461 at 500.402 kb on + strand, within GFF461 at 500.523 kb on + strand, within GFF461 at 500.524 kb on - strand, within GFF461 at 501.170 kb on - strand, within GFF462 at 501.187 kb on + strand, within GFF462 at 501.187 kb on + strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.460 kb on + strand, within GFF462 at 501.460 kb on + strand, within GFF462 at 501.601 kb on + strand, within GFF462 at 501.665 kb on - strand, within GFF462 at 502.136 kb on + strand at 502.137 kb on - strand at 502.137 kb on - strand at 502.142 kb on + strand at 502.142 kb on + strand at 502.142 kb on + strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.161 kb on - strand at 502.289 kb on + strand, within GFF463 at 502.290 kb on - strand, within GFF463 at 502.763 kb on + strand, within GFF463 at 502.763 kb on + strand, within GFF463 at 502.764 kb on - strand, within GFF463
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 500,039 + +0.1 500,040 - -0.4 500,148 + -0.4 500,148 + +1.1 500,148 + -1.2 500,149 - -0.7 500,149 - +1.5 500,151 + +0.1 500,151 + -0.0 500,151 + -0.8 500,151 + +0.2 500,151 + +0.0 500,151 + -0.2 500,152 - +0.5 500,152 - -0.6 500,152 - +0.2 500,152 - -0.2 500,152 - +0.2 500,222 + +1.5 500,222 + -0.5 500,222 + -0.8 500,222 + -0.2 500,223 - +0.4 500,223 - +1.9 500,223 - +0.2 500,223 - -2.1 500,223 - -0.4 500,223 - -0.2 500,228 + +0.1 500,228 + -0.6 500,229 - -0.2 500,402 + GFF461 0.31 +1.2 500,402 + GFF461 0.31 -0.3 500,523 + GFF461 0.46 -1.3 500,524 - GFF461 0.46 +0.4 501,170 - GFF462 0.22 +0.7 501,187 + GFF462 0.24 +0.1 501,187 + GFF462 0.24 +1.2 501,188 - GFF462 0.24 -0.9 501,188 - GFF462 0.24 -1.1 501,188 - GFF462 0.24 -0.6 501,219 + GFF462 0.27 -0.3 501,219 + GFF462 0.27 -0.8 501,219 + GFF462 0.27 -1.1 501,219 + GFF462 0.27 +0.7 501,220 - GFF462 0.27 -0.7 501,220 - GFF462 0.27 +0.8 501,220 - GFF462 0.27 +0.1 501,220 - GFF462 0.27 -1.9 501,220 - GFF462 0.27 -1.3 501,220 - GFF462 0.27 -0.5 501,220 - GFF462 0.27 -1.1 501,220 - GFF462 0.27 -0.2 501,289 + GFF462 0.34 -1.6 501,289 + GFF462 0.34 -0.6 501,289 + GFF462 0.34 +0.3 501,289 + GFF462 0.34 +0.4 501,289 + GFF462 0.34 -0.4 501,289 + GFF462 0.34 -1.4 501,289 + GFF462 0.34 +0.6 501,289 + GFF462 0.34 -0.1 501,290 - GFF462 0.34 +0.6 501,290 - GFF462 0.34 -2.2 501,290 - GFF462 0.34 +0.2 501,290 - GFF462 0.34 -1.8 501,290 - GFF462 0.34 -0.6 501,290 - GFF462 0.34 -1.0 501,290 - GFF462 0.34 -0.5 501,460 + GFF462 0.51 +1.0 501,460 + GFF462 0.51 -0.8 501,601 + GFF462 0.66 -0.8 501,665 - GFF462 0.72 +0.4 502,136 + +0.7 502,137 - -0.4 502,137 - +0.6 502,142 + -0.6 502,142 + -1.1 502,142 + +0.0 502,143 - -0.6 502,143 - -0.2 502,143 - -0.6 502,143 - -1.6 502,161 - -1.0 502,289 + GFF463 0.17 -0.4 502,290 - GFF463 0.17 +0.3 502,763 + GFF463 0.49 -0.7 502,763 + GFF463 0.49 -0.2 502,764 - GFF463 0.49 -0.4
Or see this region's nucleotide sequence