Strain Fitness in Variovorax sp. SCN45 around GFF459

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF458 and GFF459 are separated by 61 nucleotidesGFF459 and GFF460 overlap by 4 nucleotides GFF458 - Aldoxime dehydratase, at 496,042 to 497,097 GFF458 GFF459 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily / DUF3471 domain, at 497,159 to 498,604 GFF459 GFF460 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 498,601 to 500,139 GFF460 Position (kb) 497 498 499Strain fitness (log2 ratio) -2 -1 0 1 2 3at 496.291 kb on + strand, within GFF458at 496.291 kb on + strand, within GFF458at 496.292 kb on - strand, within GFF458at 496.333 kb on + strand, within GFF458at 496.412 kb on + strand, within GFF458at 496.412 kb on + strand, within GFF458at 496.413 kb on - strand, within GFF458at 496.552 kb on + strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.817 kb on - strand, within GFF458at 496.828 kb on + strand, within GFF458at 496.966 kb on + strand, within GFF458at 496.966 kb on + strand, within GFF458at 497.006 kb on - strandat 497.006 kb on - strandat 497.044 kb on + strandat 497.045 kb on - strandat 497.045 kb on - strandat 497.045 kb on - strandat 497.045 kb on - strandat 497.050 kb on + strandat 497.050 kb on + strandat 497.051 kb on - strandat 497.051 kb on - strandat 497.051 kb on - strandat 497.051 kb on - strandat 497.121 kb on + strandat 497.121 kb on + strandat 497.121 kb on + strandat 497.121 kb on + strandat 497.122 kb on - strandat 497.136 kb on + strandat 497.267 kb on + strandat 497.267 kb on + strandat 497.593 kb on + strand, within GFF459at 497.593 kb on + strand, within GFF459at 497.593 kb on + strand, within GFF459at 497.593 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.618 kb on + strand, within GFF459at 497.619 kb on - strand, within GFF459at 497.619 kb on - strand, within GFF459at 498.041 kb on + strand, within GFF459at 498.041 kb on + strand, within GFF459at 498.042 kb on - strand, within GFF459at 498.161 kb on + strand, within GFF459at 498.161 kb on + strand, within GFF459at 498.162 kb on - strand, within GFF459at 498.320 kb on + strand, within GFF459at 498.320 kb on - strand, within GFF459at 498.321 kb on - strand, within GFF459at 498.321 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.333 kb on - strand, within GFF459at 498.348 kb on - strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.431 kb on + strand, within GFF459at 498.501 kb on - strandat 498.866 kb on + strand, within GFF460at 498.867 kb on - strand, within GFF460at 498.887 kb on + strand, within GFF460at 498.896 kb on + strand, within GFF460at 498.896 kb on + strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.897 kb on - strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 498.944 kb on + strand, within GFF460at 499.034 kb on + strand, within GFF460at 499.034 kb on + strand, within GFF460at 499.035 kb on - strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.554 kb on - strand, within GFF460at 499.554 kb on - strand, within GFF460

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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496,291 + GFF458 0.24 -1.3
496,291 + GFF458 0.24 +0.1
496,292 - GFF458 0.24 +0.6
496,333 + GFF458 0.28 -0.4
496,412 + GFF458 0.35 -0.2
496,412 + GFF458 0.35 +0.7
496,413 - GFF458 0.35 -1.4
496,552 + GFF458 0.48 +0.1
496,742 - GFF458 0.66 -0.2
496,742 - GFF458 0.66 +0.1
496,742 - GFF458 0.66 +0.2
496,742 - GFF458 0.66 +1.2
496,817 - GFF458 0.73 +0.0
496,828 + GFF458 0.74 +0.4
496,966 + GFF458 0.88 +0.6
496,966 + GFF458 0.88 -0.4
497,006 - +2.8
497,006 - -0.1
497,044 + +0.5
497,045 - -0.0
497,045 - -0.9
497,045 - +0.4
497,045 - +0.7
497,050 + +0.0
497,050 + +0.4
497,051 - -0.0
497,051 - +0.1
497,051 - +0.1
497,051 - -0.4
497,121 + -0.3
497,121 + +0.5
497,121 + -0.6
497,121 + +1.2
497,122 - +0.2
497,136 + -0.2
497,267 + +2.5
497,267 + +0.3
497,593 + GFF459 0.30 -0.1
497,593 + GFF459 0.30 -0.2
497,593 + GFF459 0.30 -0.1
497,593 + GFF459 0.30 +0.6
497,618 + GFF459 0.32 -0.3
497,618 + GFF459 0.32 -0.7
497,618 + GFF459 0.32 -0.0
497,618 + GFF459 0.32 -0.7
497,618 + GFF459 0.32 -0.2
497,619 - GFF459 0.32 -1.0
497,619 - GFF459 0.32 +0.7
498,041 + GFF459 0.61 -0.5
498,041 + GFF459 0.61 -2.5
498,042 - GFF459 0.61 +0.1
498,161 + GFF459 0.69 -0.8
498,161 + GFF459 0.69 +0.1
498,162 - GFF459 0.69 +1.2
498,320 + GFF459 0.80 -0.5
498,320 - GFF459 0.80 -0.6
498,321 - GFF459 0.80 -1.1
498,321 - GFF459 0.80 +0.4
498,333 - GFF459 0.81 -1.1
498,333 - GFF459 0.81 +0.6
498,333 - GFF459 0.81 -0.0
498,348 - GFF459 0.82 -0.8
498,431 + GFF459 0.88 +0.7
498,431 + GFF459 0.88 -2.0
498,431 + GFF459 0.88 -0.3
498,501 - +0.5
498,866 + GFF460 0.17 -0.7
498,867 - GFF460 0.17 +0.6
498,887 + GFF460 0.19 -0.5
498,896 + GFF460 0.19 -0.0
498,896 + GFF460 0.19 -0.8
498,897 - GFF460 0.19 +0.2
498,897 - GFF460 0.19 -0.1
498,897 - GFF460 0.19 -0.4
498,897 - GFF460 0.19 -0.3
498,897 - GFF460 0.19 +1.2
498,944 + GFF460 0.22 -0.4
498,944 + GFF460 0.22 +1.6
498,944 + GFF460 0.22 -0.7
498,944 + GFF460 0.22 +0.3
499,034 + GFF460 0.28 +0.4
499,034 + GFF460 0.28 +0.7
499,035 - GFF460 0.28 +0.1
499,184 + GFF460 0.38 +1.3
499,184 + GFF460 0.38 +0.0
499,184 + GFF460 0.38 +0.1
499,185 - GFF460 0.38 -0.0
499,185 - GFF460 0.38 +0.2
499,185 - GFF460 0.38 +0.1
499,185 - GFF460 0.38 +0.0
499,185 - GFF460 0.38 -1.1
499,364 + GFF460 0.50 -0.3
499,364 + GFF460 0.50 -0.2
499,364 + GFF460 0.50 -0.6
499,364 + GFF460 0.50 +0.2
499,365 - GFF460 0.50 -0.3
499,365 - GFF460 0.50 -0.3
499,365 - GFF460 0.50 -0.6
499,365 - GFF460 0.50 +0.2
499,365 - GFF460 0.50 -0.9
499,553 + GFF460 0.62 +0.0
499,553 + GFF460 0.62 -0.5
499,554 - GFF460 0.62 -1.0
499,554 - GFF460 0.62 -0.5

Or see this region's nucleotide sequence