Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4547 and GFF4548 are separated by 217 nucleotides GFF4548 and GFF4549 are separated by 71 nucleotides GFF4549 and GFF4550 are separated by 36 nucleotides
GFF4547 - no description, at 184,294 to 185,334
GFF4547
GFF4548 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 185,552 to 186,070
GFF4548
GFF4549 - Cysteine synthase (EC 2.5.1.47), at 186,142 to 187,062
GFF4549
GFF4550 - NAD(FAD)-utilizing dehydrogenase, sll0175 homolog, at 187,099 to 188,784
GFF4550
Position (kb) 186
187
188 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 185.335 kb on - strand at 185.335 kb on - strand at 185.357 kb on + strand at 185.357 kb on + strand at 185.357 kb on + strand at 185.357 kb on + strand at 185.358 kb on - strand at 185.358 kb on - strand at 185.358 kb on - strand at 185.358 kb on - strand at 185.385 kb on + strand at 185.385 kb on + strand at 185.386 kb on - strand at 185.386 kb on - strand at 185.386 kb on - strand at 185.399 kb on + strand at 185.400 kb on - strand at 185.485 kb on + strand at 185.485 kb on + strand at 185.485 kb on + strand at 185.486 kb on - strand at 185.486 kb on - strand at 185.495 kb on + strand at 185.496 kb on - strand at 185.496 kb on - strand at 185.509 kb on + strand at 185.510 kb on - strand at 185.510 kb on - strand at 185.510 kb on - strand at 185.762 kb on + strand, within GFF4548 at 185.762 kb on + strand, within GFF4548 at 185.763 kb on - strand, within GFF4548 at 185.763 kb on - strand, within GFF4548 at 185.763 kb on - strand, within GFF4548 at 186.017 kb on + strand, within GFF4548 at 186.017 kb on + strand, within GFF4548 at 186.018 kb on - strand, within GFF4548 at 186.018 kb on - strand, within GFF4548 at 186.400 kb on + strand, within GFF4549 at 186.400 kb on + strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.401 kb on - strand, within GFF4549 at 186.463 kb on + strand, within GFF4549 at 187.012 kb on + strand at 187.012 kb on + strand at 187.012 kb on + strand at 187.013 kb on - strand at 187.013 kb on - strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.030 kb on + strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.031 kb on - strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.116 kb on + strand at 187.117 kb on - strand at 187.117 kb on - strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.120 kb on + strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.121 kb on - strand at 187.129 kb on + strand at 187.129 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.252 kb on + strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.253 kb on - strand at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.297 kb on + strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.298 kb on - strand, within GFF4550 at 187.396 kb on + strand, within GFF4550 at 187.396 kb on + strand, within GFF4550 at 187.397 kb on - strand, within GFF4550 at 187.397 kb on - strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.645 kb on + strand, within GFF4550 at 187.646 kb on - strand, within GFF4550 at 187.646 kb on - strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.729 kb on + strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.730 kb on - strand, within GFF4550 at 187.848 kb on + strand, within GFF4550
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 185,335 - +0.2 185,335 - -0.6 185,357 + +0.5 185,357 + -0.4 185,357 + -0.0 185,357 + -2.3 185,358 - -0.2 185,358 - -1.1 185,358 - -0.1 185,358 - -0.3 185,385 + -0.9 185,385 + -2.4 185,386 - -0.3 185,386 - -0.5 185,386 - -1.2 185,399 + -0.3 185,400 - +0.4 185,485 + -0.4 185,485 + -0.2 185,485 + -0.1 185,486 - +0.1 185,486 - -1.2 185,495 + +0.8 185,496 - -0.4 185,496 - -0.1 185,509 + -0.4 185,510 - -0.4 185,510 - +0.7 185,510 - -0.6 185,762 + GFF4548 0.40 -0.9 185,762 + GFF4548 0.40 +0.3 185,763 - GFF4548 0.41 +0.6 185,763 - GFF4548 0.41 -0.6 185,763 - GFF4548 0.41 +0.4 186,017 + GFF4548 0.90 -0.7 186,017 + GFF4548 0.90 -0.0 186,018 - GFF4548 0.90 +1.3 186,018 - GFF4548 0.90 -1.0 186,400 + GFF4549 0.28 +0.9 186,400 + GFF4549 0.28 -0.8 186,401 - GFF4549 0.28 +0.1 186,401 - GFF4549 0.28 +0.1 186,401 - GFF4549 0.28 -0.3 186,401 - GFF4549 0.28 +0.4 186,401 - GFF4549 0.28 +0.2 186,401 - GFF4549 0.28 +0.4 186,463 + GFF4549 0.35 +4.7 187,012 + +0.2 187,012 + -0.3 187,012 + -0.7 187,013 - -0.1 187,013 - +0.4 187,030 + -1.1 187,030 + +0.6 187,030 + -0.4 187,030 + +0.0 187,030 + -0.3 187,031 - -2.0 187,031 - -0.9 187,031 - -0.3 187,031 - +0.5 187,031 - +1.4 187,031 - -0.2 187,031 - -1.7 187,116 + +0.4 187,116 + +0.0 187,116 + -0.4 187,116 + -2.0 187,117 - -0.1 187,117 - -1.0 187,120 + +0.3 187,120 + -0.7 187,120 + -1.0 187,120 + +0.3 187,120 + -0.4 187,120 + +0.0 187,120 + -0.1 187,120 + +0.3 187,120 + -0.1 187,120 + +0.0 187,120 + -1.1 187,120 + -0.5 187,121 - +1.6 187,121 - +0.3 187,121 - -1.1 187,121 - +0.1 187,121 - +0.3 187,121 - +0.1 187,121 - +0.1 187,121 - +0.3 187,121 - -0.0 187,121 - +1.7 187,129 + +0.5 187,129 + -1.5 187,252 + +0.4 187,252 + +2.3 187,252 + +0.0 187,252 + -0.4 187,252 + -0.0 187,252 + -0.7 187,252 + -0.1 187,253 - -0.3 187,253 - -0.3 187,253 - -2.7 187,253 - +0.7 187,253 - -0.7 187,253 - +0.3 187,297 + GFF4550 0.12 -0.3 187,297 + GFF4550 0.12 -0.1 187,297 + GFF4550 0.12 -0.3 187,297 + GFF4550 0.12 -0.4 187,297 + GFF4550 0.12 +0.6 187,297 + GFF4550 0.12 -0.0 187,297 + GFF4550 0.12 +0.2 187,297 + GFF4550 0.12 +0.4 187,298 - GFF4550 0.12 -0.3 187,298 - GFF4550 0.12 -0.3 187,298 - GFF4550 0.12 +0.1 187,298 - GFF4550 0.12 +0.7 187,298 - GFF4550 0.12 +0.6 187,298 - GFF4550 0.12 +0.1 187,298 - GFF4550 0.12 +0.3 187,298 - GFF4550 0.12 +0.0 187,298 - GFF4550 0.12 -0.6 187,298 - GFF4550 0.12 +0.1 187,298 - GFF4550 0.12 +1.3 187,298 - GFF4550 0.12 +0.3 187,298 - GFF4550 0.12 +0.2 187,298 - GFF4550 0.12 -1.3 187,396 + GFF4550 0.18 -0.0 187,396 + GFF4550 0.18 +1.7 187,397 - GFF4550 0.18 +0.7 187,397 - GFF4550 0.18 +0.5 187,645 + GFF4550 0.32 +0.2 187,645 + GFF4550 0.32 +0.2 187,645 + GFF4550 0.32 -0.5 187,645 + GFF4550 0.32 +0.4 187,645 + GFF4550 0.32 -0.7 187,645 + GFF4550 0.32 -0.4 187,646 - GFF4550 0.32 -0.5 187,646 - GFF4550 0.32 +1.5 187,729 + GFF4550 0.37 +0.9 187,729 + GFF4550 0.37 +0.1 187,729 + GFF4550 0.37 -0.0 187,729 + GFF4550 0.37 -0.1 187,730 - GFF4550 0.37 +1.1 187,730 - GFF4550 0.37 +0.2 187,730 - GFF4550 0.37 +1.0 187,848 + GFF4550 0.44 +0.9
Or see this region's nucleotide sequence