Strain Fitness in Variovorax sp. SCN45 around GFF4500

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
143,316	+		GFF4498	0.70	-0.1
143,316	+		GFF4498	0.70	+0.9
143,317	-		GFF4498	0.70	-2.4
143,317	-		GFF4498	0.70	+0.3
143,361	+		GFF4498	0.72	+0.1
143,361	+		GFF4498	0.72	+1.2
143,361	+		GFF4498	0.72	+1.0
143,362	-		GFF4498	0.72	-0.1
143,362	-		GFF4498	0.72	-0.6
143,362	-		GFF4498	0.72	-0.2
143,362	-		GFF4498	0.72	-0.5
144,149	+		GFF4499	0.82	-1.2
144,149	+		GFF4499	0.82	-0.1
144,149	+		GFF4499	0.82	+0.3
144,149	+		GFF4499	0.82	-0.9
144,149	+		GFF4499	0.82	-0.5
144,149	+		GFF4499	0.82	-0.6
144,149	+		GFF4499	0.82	+0.2
144,150	-		GFF4499	0.82	-0.7
144,150	-		GFF4499	0.82	+0.1
144,150	-		GFF4499	0.82	+0.4
144,150	-		GFF4499	0.82	-0.8
144,150	-		GFF4499	0.82	-0.5
144,150	-		GFF4499	0.82	+0.7
144,191	+				-1.1
144,191	+				-0.2
144,191	+				+0.7
144,191	+				-1.0
144,191	+				+0.7
144,191	+				-0.3
144,191	+				-0.3
144,191	+				+0.7
144,191	+				-0.1
144,192	-				-0.1
144,192	-				+0.5
144,192	-				-0.7
144,192	-				+0.1
144,192	-				-2.0
144,192	-				-1.1
144,192	-				+0.7
144,192	-				-0.3
144,192	-				+0.7
144,192	-				+0.3
144,253	+				+1.4
144,253	+				-1.7
144,253	+				-0.9
144,253	+				-0.6
144,253	+				+1.4
144,254	-				+2.7
144,254	-				+0.2
144,254	-				-0.2
144,254	-				-0.3
144,271	+				+1.0
144,272	-				+0.7
144,313	+		GFF4500	0.14	-0.9
144,313	+		GFF4500	0.14	+0.8
144,314	-		GFF4500	0.15	-0.1
144,314	-		GFF4500	0.15	+0.4
144,314	-		GFF4500	0.15	+0.3
144,314	-		GFF4500	0.15	-0.3
144,355	+		GFF4500	0.21	-0.8
144,355	+		GFF4500	0.21	-0.0
144,355	+		GFF4500	0.21	+0.9
144,355	+		GFF4500	0.21	+0.6
144,356	-		GFF4500	0.21	+0.1
144,356	-		GFF4500	0.21	-0.1
144,356	-		GFF4500	0.21	+0.2
144,457	+		GFF4500	0.36	-0.4
144,457	+		GFF4500	0.36	+1.1
144,457	+		GFF4500	0.36	-0.7
144,457	+		GFF4500	0.36	+0.5
144,457	+		GFF4500	0.36	-0.1
144,457	+		GFF4500	0.36	-0.5
144,458	-		GFF4500	0.37	+0.3
144,458	-		GFF4500	0.37	+0.4
144,458	-		GFF4500	0.37	-1.8
144,458	-		GFF4500	0.37	+0.1
144,458	-		GFF4500	0.37	-0.7
144,458	-		GFF4500	0.37	-0.4
144,458	-		GFF4500	0.37	-0.8
144,458	-		GFF4500	0.37	+1.3
144,458	-		GFF4500	0.37	-0.2
144,458	-		GFF4500	0.37	-0.2
144,458	-		GFF4500	0.37	+0.5
144,458	-		GFF4500	0.37	-0.7
144,458	-		GFF4500	0.37	+0.2
144,458	-		GFF4500	0.37	-0.7
144,458	-		GFF4500	0.37	+1.4
144,984	+				+0.4
145,153	+		GFF4501	0.12	-0.3
145,153	+		GFF4501	0.12	-0.2
145,153	+		GFF4501	0.12	-0.3
145,153	+		GFF4501	0.12	-0.9
145,153	+		GFF4501	0.12	+0.1
145,153	+		GFF4501	0.12	-0.4
145,154	-		GFF4501	0.12	-0.8
145,154	-		GFF4501	0.12	-2.0
145,154	-		GFF4501	0.12	-1.8
145,154	-		GFF4501	0.12	+0.5
145,154	-		GFF4501	0.12	-0.6
145,154	-		GFF4501	0.12	+0.7
145,319	-		GFF4501	0.30	-0.1