Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4492 and GFF4493 overlap by 4 nucleotides GFF4493 and GFF4494 are separated by 80 nucleotides GFF4494 and GFF4495 are separated by 14 nucleotides GFF4495 and GFF4496 are separated by 46 nucleotides
GFF4492 - Protein of unknown function DUF484, at 137,077 to 137,781
GFF4492
GFF4493 - Diaminopimelate epimerase (EC 5.1.1.7), at 137,778 to 138,662
GFF4493
GFF4494 - hypothetical protein, at 138,743 to 139,057
GFF4494
GFF4495 - Hydrolase, alpha/beta fold family, at 139,072 to 139,893
GFF4495
GFF4496 - Uncharacterized MFS-type transporter, at 139,940 to 141,517
GFF4496
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 137.803 kb on - strand at 137.803 kb on - strand at 138.672 kb on - strand at 138.672 kb on - strand at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.810 kb on + strand, within GFF4494 at 138.811 kb on - strand, within GFF4494 at 138.811 kb on - strand, within GFF4494 at 138.811 kb on - strand, within GFF4494 at 138.811 kb on - strand, within GFF4494 at 138.966 kb on + strand, within GFF4494 at 138.966 kb on + strand, within GFF4494 at 138.966 kb on + strand, within GFF4494 at 138.966 kb on + strand, within GFF4494 at 138.967 kb on - strand, within GFF4494 at 138.967 kb on - strand, within GFF4494 at 138.967 kb on - strand, within GFF4494 at 138.967 kb on - strand, within GFF4494 at 139.151 kb on + strand at 139.151 kb on + strand at 139.151 kb on + strand at 139.152 kb on - strand at 139.152 kb on - strand at 139.403 kb on + strand, within GFF4495 at 139.403 kb on + strand, within GFF4495 at 139.404 kb on - strand, within GFF4495 at 139.404 kb on - strand, within GFF4495 at 139.583 kb on + strand, within GFF4495 at 139.583 kb on + strand, within GFF4495 at 139.584 kb on - strand, within GFF4495 at 139.584 kb on - strand, within GFF4495 at 139.584 kb on - strand, within GFF4495 at 139.584 kb on - strand, within GFF4495 at 139.685 kb on + strand, within GFF4495 at 139.685 kb on + strand, within GFF4495 at 139.685 kb on + strand, within GFF4495 at 139.685 kb on + strand, within GFF4495 at 139.685 kb on + strand, within GFF4495 at 139.686 kb on - strand, within GFF4495 at 139.686 kb on - strand, within GFF4495 at 139.686 kb on - strand, within GFF4495 at 139.691 kb on + strand, within GFF4495 at 139.691 kb on + strand, within GFF4495 at 139.691 kb on + strand, within GFF4495 at 139.691 kb on + strand, within GFF4495 at 139.691 kb on + strand, within GFF4495 at 139.692 kb on - strand, within GFF4495 at 139.692 kb on - strand, within GFF4495 at 139.853 kb on + strand at 139.853 kb on + strand at 139.853 kb on + strand at 139.853 kb on + strand at 139.853 kb on + strand at 139.853 kb on + strand at 139.854 kb on - strand at 139.854 kb on - strand at 139.854 kb on - strand at 139.854 kb on - strand at 139.854 kb on - strand at 139.854 kb on - strand at 139.856 kb on + strand at 139.856 kb on + strand at 139.856 kb on + strand at 139.856 kb on + strand at 139.856 kb on + strand at 139.857 kb on - strand at 139.857 kb on - strand at 139.857 kb on - strand at 139.857 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 137,803 - -0.4 137,803 - -1.2 138,672 - -0.3 138,672 - +3.7 138,810 + GFF4494 0.21 -2.0 138,810 + GFF4494 0.21 +0.1 138,810 + GFF4494 0.21 -0.1 138,810 + GFF4494 0.21 -0.7 138,810 + GFF4494 0.21 -0.3 138,810 + GFF4494 0.21 +0.7 138,810 + GFF4494 0.21 -1.5 138,810 + GFF4494 0.21 -0.7 138,810 + GFF4494 0.21 -0.3 138,810 + GFF4494 0.21 -0.3 138,811 - GFF4494 0.22 +0.6 138,811 - GFF4494 0.22 +0.8 138,811 - GFF4494 0.22 -0.4 138,811 - GFF4494 0.22 -0.3 138,966 + GFF4494 0.71 +0.0 138,966 + GFF4494 0.71 +1.3 138,966 + GFF4494 0.71 +1.7 138,966 + GFF4494 0.71 +0.3 138,967 - GFF4494 0.71 +0.2 138,967 - GFF4494 0.71 -0.2 138,967 - GFF4494 0.71 +0.5 138,967 - GFF4494 0.71 +2.0 139,151 + -0.2 139,151 + -1.2 139,151 + -0.5 139,152 - +0.9 139,152 - -0.1 139,403 + GFF4495 0.40 +1.1 139,403 + GFF4495 0.40 -0.6 139,404 - GFF4495 0.40 +0.1 139,404 - GFF4495 0.40 -0.2 139,583 + GFF4495 0.62 +1.0 139,583 + GFF4495 0.62 -1.4 139,584 - GFF4495 0.62 -0.5 139,584 - GFF4495 0.62 +0.2 139,584 - GFF4495 0.62 +0.2 139,584 - GFF4495 0.62 -0.7 139,685 + GFF4495 0.75 +0.1 139,685 + GFF4495 0.75 -0.3 139,685 + GFF4495 0.75 -0.3 139,685 + GFF4495 0.75 -1.7 139,685 + GFF4495 0.75 -0.0 139,686 - GFF4495 0.75 +0.6 139,686 - GFF4495 0.75 +0.7 139,686 - GFF4495 0.75 -0.3 139,691 + GFF4495 0.75 +0.2 139,691 + GFF4495 0.75 -0.0 139,691 + GFF4495 0.75 +0.3 139,691 + GFF4495 0.75 +0.7 139,691 + GFF4495 0.75 +1.7 139,692 - GFF4495 0.75 +0.7 139,692 - GFF4495 0.75 -0.7 139,853 + +0.7 139,853 + -0.0 139,853 + -0.3 139,853 + +0.2 139,853 + +0.5 139,853 + -0.5 139,854 - +0.5 139,854 - +0.2 139,854 - +0.7 139,854 - -0.8 139,854 - -1.3 139,854 - +0.2 139,856 + +0.2 139,856 + -0.5 139,856 + +0.4 139,856 + -0.1 139,856 + +0.4 139,857 - -0.2 139,857 - -3.1 139,857 - -0.1 139,857 - -0.4
Or see this region's nucleotide sequence