Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4490 and GFF4491 overlap by 26 nucleotides GFF4491 and GFF4492 overlap by 4 nucleotides GFF4492 and GFF4493 overlap by 4 nucleotides
GFF4490 - Site-specific tyrosine recombinase XerC, at 135,454 to 136,470
GFF4490
GFF4491 - no description, at 136,445 to 137,080
GFF4491
GFF4492 - Protein of unknown function DUF484, at 137,077 to 137,781
GFF4492
GFF4493 - Diaminopimelate epimerase (EC 5.1.1.7), at 137,778 to 138,662
GFF4493
Position (kb)
136
137
138 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.451 kb on + strand at 135.452 kb on - strand at 135.452 kb on - strand at 135.452 kb on - strand at 135.452 kb on - strand at 135.455 kb on + strand at 135.455 kb on + strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.456 kb on - strand at 135.461 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.503 kb on + strand at 135.504 kb on - strand at 135.504 kb on - strand at 135.504 kb on - strand at 135.504 kb on - strand at 135.504 kb on - strand at 135.504 kb on - strand at 135.537 kb on + strand at 135.538 kb on - strand at 135.539 kb on + strand at 135.539 kb on + strand at 135.539 kb on + strand at 135.539 kb on + strand at 135.539 kb on + strand at 135.540 kb on - strand at 135.540 kb on - strand at 135.540 kb on - strand at 135.540 kb on - strand at 135.540 kb on - strand at 135.986 kb on + strand, within GFF4490 at 135.987 kb on - strand, within GFF4490 at 135.987 kb on - strand, within GFF4490 at 135.987 kb on - strand, within GFF4490 at 136.046 kb on + strand, within GFF4490 at 136.046 kb on + strand, within GFF4490 at 136.046 kb on + strand, within GFF4490 at 136.046 kb on + strand, within GFF4490 at 136.046 kb on + strand, within GFF4490 at 136.047 kb on - strand, within GFF4490 at 136.047 kb on - strand, within GFF4490 at 136.172 kb on + strand, within GFF4490 at 136.172 kb on + strand, within GFF4490 at 136.172 kb on + strand, within GFF4490 at 136.172 kb on + strand, within GFF4490 at 136.173 kb on - strand, within GFF4490 at 136.334 kb on + strand, within GFF4490 at 136.334 kb on + strand, within GFF4490 at 136.334 kb on + strand, within GFF4490 at 136.554 kb on + strand, within GFF4491 at 136.554 kb on + strand, within GFF4491 at 136.555 kb on - strand, within GFF4491 at 136.555 kb on - strand, within GFF4491 at 136.555 kb on - strand, within GFF4491 at 136.737 kb on + strand, within GFF4491 at 136.737 kb on + strand, within GFF4491 at 136.737 kb on + strand, within GFF4491 at 136.737 kb on + strand, within GFF4491 at 136.902 kb on + strand, within GFF4491 at 136.902 kb on + strand, within GFF4491 at 136.902 kb on + strand, within GFF4491 at 136.902 kb on + strand, within GFF4491 at 136.903 kb on - strand, within GFF4491 at 136.903 kb on - strand, within GFF4491 at 136.903 kb on - strand, within GFF4491 at 137.303 kb on + strand, within GFF4492 at 137.304 kb on - strand, within GFF4492 at 137.360 kb on + strand, within GFF4492 at 137.360 kb on + strand, within GFF4492 at 137.360 kb on + strand, within GFF4492 at 137.360 kb on + strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.361 kb on - strand, within GFF4492 at 137.376 kb on - strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.708 kb on + strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.709 kb on - strand, within GFF4492 at 137.803 kb on - strand at 137.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 135,451 + -0.7 135,451 + +0.0 135,451 + +0.3 135,451 + +0.2 135,451 + +0.1 135,451 + +0.4 135,451 + +0.9 135,451 + -0.5 135,451 + -0.6 135,451 + +0.0 135,451 + -0.4 135,452 - +0.0 135,452 - -1.4 135,452 - -0.2 135,452 - -0.4 135,455 + -0.0 135,455 + -0.5 135,456 - +1.7 135,456 - +0.6 135,456 - -0.5 135,456 - +0.4 135,456 - +0.1 135,456 - +0.1 135,456 - -0.1 135,461 + -0.3 135,503 + -1.5 135,503 + +0.5 135,503 + -1.7 135,503 + -1.7 135,503 + -0.3 135,503 + +0.1 135,503 + -2.0 135,503 + +0.5 135,504 - -1.1 135,504 - -0.9 135,504 - -1.7 135,504 - +0.7 135,504 - -1.1 135,504 - -1.7 135,537 + +2.0 135,538 - -1.0 135,539 + +0.1 135,539 + -0.3 135,539 + -0.1 135,539 + +0.5 135,539 + +1.1 135,540 - -1.1 135,540 - -1.1 135,540 - -1.2 135,540 - +0.7 135,540 - -0.1 135,986 + GFF4490 0.52 -0.7 135,987 - GFF4490 0.52 -0.3 135,987 - GFF4490 0.52 +0.7 135,987 - GFF4490 0.52 +1.1 136,046 + GFF4490 0.58 -0.1 136,046 + GFF4490 0.58 -0.5 136,046 + GFF4490 0.58 +0.7 136,046 + GFF4490 0.58 -0.6 136,046 + GFF4490 0.58 -1.1 136,047 - GFF4490 0.58 -1.7 136,047 - GFF4490 0.58 -1.2 136,172 + GFF4490 0.71 +0.2 136,172 + GFF4490 0.71 -0.5 136,172 + GFF4490 0.71 -1.1 136,172 + GFF4490 0.71 -1.6 136,173 - GFF4490 0.71 -1.3 136,334 + GFF4490 0.87 -2.7 136,334 + GFF4490 0.87 +0.9 136,334 + GFF4490 0.87 -0.7 136,554 + GFF4491 0.17 +1.3 136,554 + GFF4491 0.17 -0.3 136,555 - GFF4491 0.17 +0.0 136,555 - GFF4491 0.17 +0.9 136,555 - GFF4491 0.17 -1.3 136,737 + GFF4491 0.46 -0.0 136,737 + GFF4491 0.46 -1.7 136,737 + GFF4491 0.46 -0.5 136,737 + GFF4491 0.46 -0.7 136,902 + GFF4491 0.72 -0.4 136,902 + GFF4491 0.72 -0.9 136,902 + GFF4491 0.72 +0.2 136,902 + GFF4491 0.72 +0.8 136,903 - GFF4491 0.72 -0.9 136,903 - GFF4491 0.72 -0.5 136,903 - GFF4491 0.72 -1.5 137,303 + GFF4492 0.32 -0.3 137,304 - GFF4492 0.32 +0.2 137,360 + GFF4492 0.40 -1.0 137,360 + GFF4492 0.40 -0.9 137,360 + GFF4492 0.40 -0.9 137,360 + GFF4492 0.40 -0.9 137,361 - GFF4492 0.40 -2.0 137,361 - GFF4492 0.40 -0.1 137,361 - GFF4492 0.40 -0.3 137,361 - GFF4492 0.40 -0.7 137,361 - GFF4492 0.40 +0.4 137,361 - GFF4492 0.40 -0.4 137,361 - GFF4492 0.40 +0.1 137,376 - GFF4492 0.42 -0.4 137,708 + GFF4492 0.90 -0.0 137,708 + GFF4492 0.90 -0.7 137,708 + GFF4492 0.90 +0.3 137,708 + GFF4492 0.90 -0.2 137,708 + GFF4492 0.90 -0.9 137,708 + GFF4492 0.90 -2.3 137,708 + GFF4492 0.90 -0.4 137,708 + GFF4492 0.90 +1.3 137,709 - GFF4492 0.90 -1.4 137,709 - GFF4492 0.90 -0.3 137,709 - GFF4492 0.90 -0.2 137,709 - GFF4492 0.90 -1.6 137,709 - GFF4492 0.90 -1.1 137,709 - GFF4492 0.90 -0.7 137,709 - GFF4492 0.90 -0.3 137,709 - GFF4492 0.90 -0.2 137,709 - GFF4492 0.90 -0.9 137,709 - GFF4492 0.90 +0.7 137,709 - GFF4492 0.90 -0.2 137,803 - -0.4 137,803 - -1.2
Or see this region's nucleotide sequence