Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4384 and GFF4385 are separated by 265 nucleotides GFF4385 and GFF4386 overlap by 4 nucleotides GFF4386 and GFF4387 overlap by 4 nucleotides GFF4387 and GFF4388 overlap by 4 nucleotides
GFF4384 - Plasmid replication initiator protein, at 13,515 to 14,372
GFF4384
GFF4385 - ParA-like protein, at 14,638 to 15,276
GFF4385
GFF4386 - FIG00457034: hypothetical protein, at 15,273 to 15,557
GFF4386
GFF4387 - Glycosidases, at 15,554 to 16,099
GFF4387
GFF4388 - Type IV secretory pathway, protease TraF, at 16,096 to 16,695
GFF4388
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.741 kb on + strand, within GFF4384 at 13.742 kb on - strand, within GFF4384 at 13.822 kb on + strand, within GFF4384 at 13.822 kb on + strand, within GFF4384 at 13.837 kb on - strand, within GFF4384 at 13.881 kb on + strand, within GFF4384 at 13.881 kb on + strand, within GFF4384 at 13.881 kb on + strand, within GFF4384 at 13.882 kb on - strand, within GFF4384 at 13.882 kb on - strand, within GFF4384 at 13.882 kb on - strand, within GFF4384 at 13.882 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.056 kb on - strand, within GFF4384 at 14.103 kb on + strand, within GFF4384 at 14.103 kb on + strand, within GFF4384 at 14.104 kb on - strand, within GFF4384 at 14.139 kb on + strand, within GFF4384 at 14.139 kb on + strand, within GFF4384 at 14.139 kb on + strand, within GFF4384 at 14.139 kb on + strand, within GFF4384 at 14.139 kb on + strand, within GFF4384 at 14.248 kb on - strand, within GFF4384 at 14.289 kb on + strand at 14.290 kb on - strand at 14.290 kb on - strand at 14.290 kb on - strand at 14.290 kb on - strand at 14.370 kb on + strand at 14.370 kb on + strand at 14.370 kb on + strand at 14.370 kb on + strand at 14.371 kb on - strand at 14.404 kb on - strand at 14.404 kb on - strand at 14.404 kb on - strand at 14.437 kb on - strand at 14.437 kb on - strand at 14.437 kb on - strand at 14.437 kb on - strand at 14.437 kb on - strand at 14.442 kb on + strand at 14.442 kb on + strand at 14.442 kb on + strand at 14.443 kb on - strand at 14.443 kb on - strand at 14.443 kb on - strand at 14.443 kb on - strand at 14.450 kb on + strand at 14.450 kb on + strand at 14.450 kb on + strand at 14.451 kb on - strand at 14.451 kb on - strand at 14.451 kb on - strand at 14.451 kb on - strand at 14.460 kb on - strand at 14.463 kb on - strand at 14.482 kb on - strand at 14.506 kb on + strand at 14.506 kb on + strand at 14.506 kb on + strand at 14.506 kb on + strand at 14.507 kb on - strand at 14.507 kb on - strand at 14.515 kb on + strand at 14.519 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.525 kb on + strand at 14.526 kb on - strand at 14.526 kb on - strand at 14.526 kb on - strand at 14.526 kb on - strand at 14.526 kb on - strand at 14.529 kb on + strand at 14.529 kb on + strand at 14.530 kb on - strand at 14.530 kb on - strand at 14.530 kb on - strand at 14.530 kb on - strand at 14.536 kb on + strand at 14.536 kb on + strand at 14.537 kb on - strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.540 kb on - strand at 14.540 kb on - strand at 14.540 kb on - strand at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.311 kb on + strand, within GFF4386 at 15.311 kb on + strand, within GFF4386 at 15.311 kb on + strand, within GFF4386 at 15.312 kb on - strand, within GFF4386 at 15.312 kb on - strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.409 kb on - strand, within GFF4386 at 15.409 kb on - strand, within GFF4386 at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.619 kb on - strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.804 kb on - strand, within GFF4387 at 15.998 kb on + strand, within GFF4387 at 15.998 kb on + strand, within GFF4387 at 15.999 kb on - strand, within GFF4387 at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 13,741 + GFF4384 0.26 +1.0 13,742 - GFF4384 0.26 -0.7 13,822 + GFF4384 0.36 -0.2 13,822 + GFF4384 0.36 +0.3 13,837 - GFF4384 0.38 +0.7 13,881 + GFF4384 0.43 -0.5 13,881 + GFF4384 0.43 -0.7 13,881 + GFF4384 0.43 -0.6 13,882 - GFF4384 0.43 -0.2 13,882 - GFF4384 0.43 -0.4 13,882 - GFF4384 0.43 -1.8 13,882 - GFF4384 0.43 -0.0 14,056 - GFF4384 0.63 +0.0 14,056 - GFF4384 0.63 +0.8 14,056 - GFF4384 0.63 -0.3 14,056 - GFF4384 0.63 +0.3 14,056 - GFF4384 0.63 +0.8 14,056 - GFF4384 0.63 +0.1 14,056 - GFF4384 0.63 +0.9 14,056 - GFF4384 0.63 +0.6 14,056 - GFF4384 0.63 +0.3 14,056 - GFF4384 0.63 -0.4 14,056 - GFF4384 0.63 +1.7 14,056 - GFF4384 0.63 +0.6 14,056 - GFF4384 0.63 +0.9 14,103 + GFF4384 0.69 +0.5 14,103 + GFF4384 0.69 +0.3 14,104 - GFF4384 0.69 +0.5 14,139 + GFF4384 0.73 +0.7 14,139 + GFF4384 0.73 +1.9 14,139 + GFF4384 0.73 -0.5 14,139 + GFF4384 0.73 -0.5 14,139 + GFF4384 0.73 +0.2 14,248 - GFF4384 0.85 -0.4 14,289 + -0.4 14,290 - +0.2 14,290 - -0.1 14,290 - +1.7 14,290 - +0.0 14,370 + -0.3 14,370 + -0.3 14,370 + -0.3 14,370 + +0.2 14,371 - -0.1 14,404 - +0.5 14,404 - -0.7 14,404 - +0.1 14,437 - +0.5 14,437 - -1.3 14,437 - +0.1 14,437 - +0.3 14,437 - +0.4 14,442 + -0.4 14,442 + +1.2 14,442 + -0.0 14,443 - -0.1 14,443 - -0.2 14,443 - -0.0 14,443 - +1.5 14,450 + -0.4 14,450 + +0.3 14,450 + +0.0 14,451 - -0.1 14,451 - +0.7 14,451 - +1.2 14,451 - -0.6 14,460 - -0.1 14,463 - +0.2 14,482 - +1.1 14,506 + +0.2 14,506 + +0.3 14,506 + -1.0 14,506 + +0.2 14,507 - +0.5 14,507 - -1.1 14,515 + -1.0 14,519 + -0.1 14,525 + +0.4 14,525 + -1.2 14,525 + +0.9 14,525 + -1.0 14,525 + -0.4 14,525 + +1.3 14,525 + -0.2 14,525 + +1.3 14,525 + -0.1 14,525 + +0.2 14,525 + +0.5 14,525 + -0.9 14,525 + +0.5 14,525 + -0.4 14,526 - +0.1 14,526 - +0.1 14,526 - -0.3 14,526 - -0.4 14,526 - -0.2 14,529 + -0.7 14,529 + -0.3 14,530 - -0.1 14,530 - -1.0 14,530 - +1.3 14,530 - +0.5 14,536 + -1.0 14,536 + +1.1 14,537 - +1.1 14,539 + -0.4 14,539 + -0.9 14,539 + -0.0 14,539 + +0.3 14,539 + -0.4 14,539 + -0.2 14,539 + +0.2 14,539 + +0.6 14,539 + +0.7 14,539 + -0.5 14,540 - +0.1 14,540 - +0.4 14,540 - -0.4 14,970 + GFF4385 0.52 -0.3 14,970 + GFF4385 0.52 +0.7 14,970 + GFF4385 0.52 -2.7 14,970 + GFF4385 0.52 -0.3 14,970 + GFF4385 0.52 -0.2 14,970 + GFF4385 0.52 -0.5 14,970 + GFF4385 0.52 -0.7 14,977 + GFF4385 0.53 -1.7 14,977 + GFF4385 0.53 -1.1 14,977 + GFF4385 0.53 -0.1 14,977 + GFF4385 0.53 -1.5 15,078 + GFF4385 0.69 -0.4 15,078 + GFF4385 0.69 -0.4 15,078 + GFF4385 0.69 -1.3 15,078 + GFF4385 0.69 -0.0 15,079 - GFF4385 0.69 +0.8 15,079 - GFF4385 0.69 -0.8 15,079 - GFF4385 0.69 -0.3 15,079 - GFF4385 0.69 -0.0 15,079 - GFF4385 0.69 -0.2 15,079 - GFF4385 0.69 -0.7 15,311 + GFF4386 0.13 +2.3 15,311 + GFF4386 0.13 +0.1 15,311 + GFF4386 0.13 -0.2 15,312 - GFF4386 0.14 -0.9 15,312 - GFF4386 0.14 -0.4 15,408 + GFF4386 0.47 -1.3 15,408 + GFF4386 0.47 -0.6 15,408 + GFF4386 0.47 -1.6 15,408 + GFF4386 0.47 +0.1 15,408 + GFF4386 0.47 +0.1 15,408 + GFF4386 0.47 +1.7 15,409 - GFF4386 0.48 -1.9 15,409 - GFF4386 0.48 +0.8 15,606 + -0.8 15,606 + -0.5 15,606 + +0.4 15,606 + -1.1 15,606 + -0.8 15,606 + +0.6 15,606 + -0.2 15,606 + +0.1 15,606 + -0.7 15,606 + -0.1 15,606 + -0.0 15,606 + -1.8 15,606 + -0.5 15,606 + -0.2 15,607 - +0.1 15,607 - +1.3 15,607 - -0.9 15,607 - -0.0 15,607 - +1.4 15,607 - +0.5 15,607 - -0.0 15,607 - +0.9 15,607 - +1.7 15,619 - GFF4387 0.12 -0.6 15,692 + GFF4387 0.25 +0.0 15,692 + GFF4387 0.25 +0.0 15,692 + GFF4387 0.25 -0.4 15,692 + GFF4387 0.25 -0.1 15,692 + GFF4387 0.25 -0.5 15,693 - GFF4387 0.25 +0.1 15,693 - GFF4387 0.25 -1.8 15,693 - GFF4387 0.25 -0.3 15,693 - GFF4387 0.25 +0.2 15,693 - GFF4387 0.25 -0.4 15,693 - GFF4387 0.25 +0.4 15,693 - GFF4387 0.25 +0.3 15,804 - GFF4387 0.46 +1.1 15,998 + GFF4387 0.81 -0.9 15,998 + GFF4387 0.81 -1.3 15,999 - GFF4387 0.82 -0.4 16,049 + -0.6 16,049 + -0.5 16,049 + -2.1 16,049 + -2.0 16,049 + -0.5 16,049 + -0.1 16,049 + -0.3 16,049 + -1.2 16,050 - -0.6 16,050 - +0.9 16,050 - -0.6 16,050 - -0.2 16,250 - GFF4388 0.26 -1.7 16,250 - GFF4388 0.26 +1.4 16,250 - GFF4388 0.26 -0.9 16,250 - GFF4388 0.26 -0.1 16,250 - GFF4388 0.26 -1.5 16,250 - GFF4388 0.26 -1.0 16,250 - GFF4388 0.26 -0.2 16,250 - GFF4388 0.26 -0.2 16,250 - GFF4388 0.26 +0.9 16,250 - GFF4388 0.26 -0.1 16,250 - GFF4388 0.26 +0.4 16,250 - GFF4388 0.26 +0.7 16,250 - GFF4388 0.26 -1.9 16,250 - GFF4388 0.26 -0.9
Or see this region's nucleotide sequence