Strain Fitness in Variovorax sp. SCN45 around GFF4300

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
218,580	-		GFF4299	0.48	+0.4
218,747	+		GFF4299	0.62	+0.2
219,254	+				+0.6
219,281	-				-0.6
219,499	+		GFF4300	0.20	-1.6
219,500	-		GFF4300	0.20	-0.6
219,500	-		GFF4300	0.20	-0.7
219,500	-		GFF4300	0.20	-0.7
219,718	+		GFF4300	0.34	-0.3
219,719	-		GFF4300	0.34	-1.2
219,719	-		GFF4300	0.34	-0.4
219,719	-		GFF4300	0.34	+0.2
220,069	+		GFF4300	0.58	-1.3
220,612	-				+0.2
220,695	+				-0.2
220,722	+				+0.2
221,270	-		GFF4301	0.48	-0.6
221,594	-		GFF4301	0.83	+0.1