Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4257 and GFF4258 overlap by 8 nucleotides GFF4258 and GFF4259 are separated by 34 nucleotides GFF4259 and GFF4260 are separated by 154 nucleotides
GFF4257 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 172,605 to 173,741
GFF4257
GFF4258 - no description, at 173,734 to 174,105
GFF4258
GFF4259 - Transcriptional regulator, DeoR family, at 174,140 to 174,862
GFF4259
GFF4260 - hypothetical protein, at 175,017 to 179,039
GFF4260
Position (kb) 173
174
175 Strain fitness (log2 ratio) -2
-1
0
1 at 173.310 kb on + strand, within GFF4257 at 173.310 kb on + strand, within GFF4257 at 173.311 kb on - strand, within GFF4257 at 173.319 kb on + strand, within GFF4257 at 173.320 kb on - strand, within GFF4257 at 173.320 kb on - strand, within GFF4257 at 173.453 kb on - strand, within GFF4257 at 173.490 kb on + strand, within GFF4257 at 173.490 kb on + strand, within GFF4257 at 173.491 kb on - strand, within GFF4257 at 173.739 kb on + strand at 173.739 kb on + strand at 173.740 kb on - strand at 173.740 kb on - strand at 173.861 kb on + strand, within GFF4258 at 173.861 kb on + strand, within GFF4258 at 174.047 kb on + strand, within GFF4258 at 174.048 kb on - strand, within GFF4258 at 174.048 kb on - strand, within GFF4258 at 174.048 kb on - strand, within GFF4258 at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.141 kb on + strand at 174.142 kb on - strand at 174.142 kb on - strand at 174.142 kb on - strand at 174.170 kb on + strand at 174.170 kb on + strand at 174.171 kb on - strand at 174.171 kb on - strand at 174.171 kb on - strand at 174.400 kb on - strand, within GFF4259 at 174.687 kb on + strand, within GFF4259 at 174.688 kb on - strand, within GFF4259 at 174.874 kb on + strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.875 kb on - strand at 174.950 kb on + strand at 174.950 kb on + strand at 174.950 kb on + strand at 174.950 kb on + strand at 174.950 kb on + strand at 174.950 kb on + strand at 174.951 kb on - strand at 174.951 kb on - strand at 174.951 kb on - strand at 174.951 kb on - strand at 174.951 kb on - strand at 174.954 kb on - strand at 174.954 kb on - strand at 174.967 kb on - strand at 174.981 kb on + strand at 174.981 kb on + strand at 174.982 kb on - strand at 175.000 kb on + strand at 175.000 kb on + strand at 175.000 kb on + strand at 175.000 kb on + strand at 175.000 kb on + strand at 175.001 kb on - strand at 175.001 kb on - strand at 175.001 kb on - strand at 175.029 kb on + strand at 175.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 173,310 + GFF4257 0.62 -0.2 173,310 + GFF4257 0.62 -1.2 173,311 - GFF4257 0.62 -0.6 173,319 + GFF4257 0.63 -1.5 173,320 - GFF4257 0.63 -2.7 173,320 - GFF4257 0.63 -2.4 173,453 - GFF4257 0.75 +0.2 173,490 + GFF4257 0.78 -0.4 173,490 + GFF4257 0.78 -1.9 173,491 - GFF4257 0.78 -0.5 173,739 + -1.0 173,739 + -0.5 173,740 - +0.1 173,740 - -0.3 173,861 + GFF4258 0.34 +0.3 173,861 + GFF4258 0.34 +0.2 174,047 + GFF4258 0.84 +0.3 174,048 - GFF4258 0.84 -0.2 174,048 - GFF4258 0.84 +0.8 174,048 - GFF4258 0.84 -1.2 174,141 + -0.1 174,141 + -1.0 174,141 + +0.3 174,141 + -0.7 174,141 + -0.2 174,141 + -0.3 174,141 + +0.6 174,141 + +0.1 174,141 + +0.2 174,142 - +0.1 174,142 - +0.4 174,142 - -0.1 174,170 + -0.1 174,170 + -1.2 174,171 - -0.7 174,171 - -0.3 174,171 - -0.3 174,400 - GFF4259 0.36 +0.1 174,687 + GFF4259 0.76 -0.5 174,688 - GFF4259 0.76 -2.0 174,874 + -0.1 174,875 - -0.3 174,875 - +0.2 174,875 - +1.0 174,875 - +0.4 174,875 - -1.2 174,875 - +0.0 174,875 - +1.1 174,950 + -0.5 174,950 + -0.1 174,950 + -1.7 174,950 + -1.9 174,950 + -0.6 174,950 + -0.8 174,951 - -0.2 174,951 - -0.6 174,951 - -0.5 174,951 - -0.5 174,951 - -0.1 174,954 - +0.2 174,954 - -1.0 174,967 - -0.1 174,981 + +0.0 174,981 + -0.2 174,982 - +0.2 175,000 + -0.4 175,000 + -0.4 175,000 + -1.0 175,000 + +0.3 175,000 + -0.8 175,001 - +1.0 175,001 - -1.2 175,001 - -1.0 175,029 + +0.2 175,029 + -1.9
Or see this region's nucleotide sequence