Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4223 and GFF4225 are separated by 83 nucleotides GFF4225 and GFF4224 overlap by 2 nucleotides GFF4224 and GFF4226 are separated by 54 nucleotides
GFF4223 - BUG/TctC family periplasmic protein, at 135,802 to 136,788
GFF4223
GFF4225 - no description, at 136,872 to 137,039
GFF4225
GFF4224 - Transcriptional regulator, AcrR family, at 137,038 to 137,670
GFF4224
GFF4226 - Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84), at 137,725 to 138,714
GFF4226
Position (kb) 137
138 Strain fitness (log2 ratio) -2
-1
0
1
2 at 136.230 kb on - strand, within GFF4223 at 136.230 kb on - strand, within GFF4223 at 136.423 kb on + strand, within GFF4223 at 136.423 kb on + strand, within GFF4223 at 136.423 kb on + strand, within GFF4223 at 136.423 kb on + strand, within GFF4223 at 136.424 kb on - strand, within GFF4223 at 136.424 kb on - strand, within GFF4223 at 136.424 kb on - strand, within GFF4223 at 136.424 kb on - strand, within GFF4223 at 136.424 kb on - strand, within GFF4223 at 136.427 kb on + strand, within GFF4223 at 136.427 kb on + strand, within GFF4223 at 136.427 kb on + strand, within GFF4223 at 136.704 kb on - strand at 136.772 kb on + strand at 136.773 kb on - strand at 136.773 kb on - strand at 136.831 kb on + strand at 137.090 kb on - strand at 137.090 kb on - strand at 137.209 kb on + strand, within GFF4224 at 137.209 kb on + strand, within GFF4224 at 137.209 kb on + strand, within GFF4224 at 137.209 kb on + strand, within GFF4224 at 137.213 kb on - strand, within GFF4224 at 137.215 kb on + strand, within GFF4224 at 137.216 kb on - strand, within GFF4224 at 137.216 kb on - strand, within GFF4224 at 137.260 kb on + strand, within GFF4224 at 137.269 kb on + strand, within GFF4224 at 137.438 kb on - strand, within GFF4224 at 137.833 kb on + strand, within GFF4226 at 137.833 kb on + strand, within GFF4226 at 137.833 kb on + strand, within GFF4226 at 137.834 kb on - strand, within GFF4226 at 137.848 kb on + strand, within GFF4226 at 138.155 kb on + strand, within GFF4226 at 138.155 kb on + strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.283 kb on + strand, within GFF4226 at 138.283 kb on + strand, within GFF4226 at 138.284 kb on - strand, within GFF4226
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 136,230 - GFF4223 0.43 +1.9 136,230 - GFF4223 0.43 +0.3 136,423 + GFF4223 0.63 -0.4 136,423 + GFF4223 0.63 +0.3 136,423 + GFF4223 0.63 +0.0 136,423 + GFF4223 0.63 +0.4 136,424 - GFF4223 0.63 -0.7 136,424 - GFF4223 0.63 +0.6 136,424 - GFF4223 0.63 -0.2 136,424 - GFF4223 0.63 -0.4 136,424 - GFF4223 0.63 +0.4 136,427 + GFF4223 0.63 +0.8 136,427 + GFF4223 0.63 -0.2 136,427 + GFF4223 0.63 +0.3 136,704 - -0.2 136,772 + -0.7 136,773 - -0.7 136,773 - -0.6 136,831 + -0.2 137,090 - +0.6 137,090 - -0.4 137,209 + GFF4224 0.27 -1.3 137,209 + GFF4224 0.27 -0.2 137,209 + GFF4224 0.27 -1.3 137,209 + GFF4224 0.27 -0.2 137,213 - GFF4224 0.28 -0.5 137,215 + GFF4224 0.28 -0.4 137,216 - GFF4224 0.28 -1.6 137,216 - GFF4224 0.28 -0.2 137,260 + GFF4224 0.35 -0.0 137,269 + GFF4224 0.36 +0.8 137,438 - GFF4224 0.63 -1.0 137,833 + GFF4226 0.11 +0.1 137,833 + GFF4226 0.11 -0.5 137,833 + GFF4226 0.11 +0.2 137,834 - GFF4226 0.11 -0.3 137,848 + GFF4226 0.12 -0.1 138,155 + GFF4226 0.43 -1.9 138,155 + GFF4226 0.43 -0.3 138,156 - GFF4226 0.44 +0.3 138,156 - GFF4226 0.44 +0.5 138,156 - GFF4226 0.44 -0.3 138,283 + GFF4226 0.56 +0.1 138,283 + GFF4226 0.56 +1.1 138,284 - GFF4226 0.56 -0.4
Or see this region's nucleotide sequence