Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4174 and GFF4175 are separated by 45 nucleotides GFF4175 and GFF4176 are separated by 90 nucleotides GFF4176 and GFF4177 overlap by 4 nucleotides GFF4177 and GFF4178 are separated by 11 nucleotides
GFF4174 - FIG00919961: hypothetical protein, at 79,327 to 80,310
GFF4174
GFF4175 - FMN reductase (NADH) (EC 1.5.1.42), at 80,356 to 80,949
GFF4175
GFF4176 - Transcriptional activator MetR, at 81,040 to 81,939
GFF4176
GFF4177 - Epoxyqueuosine reductase (EC 1.17.99.6) QueH, at 81,936 to 82,160
GFF4177
GFF4178 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 82,172 to 83,029
GFF4178
Position (kb) 81
82 Strain fitness (log2 ratio) -1
0
1
2 at 80.108 kb on + strand, within GFF4174 at 80.109 kb on - strand, within GFF4174 at 80.212 kb on + strand at 80.212 kb on + strand at 80.213 kb on - strand at 80.213 kb on - strand at 80.258 kb on + strand at 80.258 kb on + strand at 80.258 kb on + strand at 80.338 kb on + strand at 80.338 kb on + strand at 80.338 kb on + strand at 80.339 kb on - strand at 80.339 kb on - strand at 80.339 kb on - strand at 80.339 kb on - strand at 80.675 kb on + strand, within GFF4175 at 80.675 kb on + strand, within GFF4175 at 80.676 kb on - strand, within GFF4175 at 80.676 kb on - strand, within GFF4175 at 80.676 kb on - strand, within GFF4175 at 80.917 kb on + strand at 80.918 kb on - strand at 80.918 kb on - strand at 80.918 kb on - strand at 80.996 kb on + strand at 81.230 kb on - strand, within GFF4176 at 81.349 kb on + strand, within GFF4176 at 81.349 kb on + strand, within GFF4176 at 81.529 kb on + strand, within GFF4176 at 81.529 kb on + strand, within GFF4176 at 81.530 kb on - strand, within GFF4176 at 81.581 kb on - strand, within GFF4176 at 81.623 kb on - strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.752 kb on + strand, within GFF4176 at 81.784 kb on + strand, within GFF4176 at 81.784 kb on + strand, within GFF4176 at 81.883 kb on + strand at 81.883 kb on + strand at 81.884 kb on - strand at 81.966 kb on + strand, within GFF4177 at 81.966 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 82.065 kb on + strand, within GFF4177 at 82.066 kb on - strand, within GFF4177 at 82.066 kb on - strand, within GFF4177 at 82.206 kb on - strand at 82.284 kb on - strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.785 kb on + strand, within GFF4178 at 82.786 kb on - strand, within GFF4178 at 82.796 kb on + strand, within GFF4178 at 82.844 kb on + strand, within GFF4178
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 80,108 + GFF4174 0.79 +0.1 80,109 - GFF4174 0.79 -0.2 80,212 + -0.6 80,212 + +1.2 80,213 - -0.7 80,213 - -0.0 80,258 + -0.6 80,258 + +0.7 80,258 + +2.1 80,338 + +0.0 80,338 + +0.8 80,338 + +0.2 80,339 - +0.1 80,339 - +1.3 80,339 - -1.1 80,339 - -0.0 80,675 + GFF4175 0.54 +1.8 80,675 + GFF4175 0.54 +0.2 80,676 - GFF4175 0.54 -0.6 80,676 - GFF4175 0.54 +0.5 80,676 - GFF4175 0.54 -1.2 80,917 + +0.5 80,918 - -0.8 80,918 - +0.6 80,918 - +0.6 80,996 + -1.2 81,230 - GFF4176 0.21 -0.4 81,349 + GFF4176 0.34 -1.2 81,349 + GFF4176 0.34 +2.2 81,529 + GFF4176 0.54 -0.2 81,529 + GFF4176 0.54 -0.0 81,530 - GFF4176 0.54 -1.4 81,581 - GFF4176 0.60 -0.9 81,623 - GFF4176 0.65 -1.2 81,652 + GFF4176 0.68 -0.5 81,652 + GFF4176 0.68 +0.1 81,652 + GFF4176 0.68 +0.4 81,652 + GFF4176 0.68 +0.1 81,652 + GFF4176 0.68 +0.5 81,652 + GFF4176 0.68 -0.0 81,652 + GFF4176 0.68 +0.2 81,652 + GFF4176 0.68 +0.1 81,652 + GFF4176 0.68 +0.6 81,652 + GFF4176 0.68 +0.6 81,652 + GFF4176 0.68 -0.2 81,652 + GFF4176 0.68 -0.3 81,652 + GFF4176 0.68 +0.7 81,652 + GFF4176 0.68 -0.9 81,653 - GFF4176 0.68 -0.3 81,653 - GFF4176 0.68 -0.6 81,653 - GFF4176 0.68 +0.5 81,653 - GFF4176 0.68 +0.2 81,653 - GFF4176 0.68 +0.3 81,653 - GFF4176 0.68 +0.3 81,653 - GFF4176 0.68 +1.6 81,653 - GFF4176 0.68 +0.3 81,653 - GFF4176 0.68 +1.6 81,653 - GFF4176 0.68 +0.4 81,653 - GFF4176 0.68 +0.3 81,653 - GFF4176 0.68 -1.1 81,653 - GFF4176 0.68 +1.3 81,653 - GFF4176 0.68 -0.7 81,752 + GFF4176 0.79 +1.1 81,784 + GFF4176 0.83 -0.9 81,784 + GFF4176 0.83 +0.3 81,883 + +0.2 81,883 + +0.6 81,884 - -0.4 81,966 + GFF4177 0.13 -0.3 81,966 + GFF4177 0.13 +0.7 81,978 + GFF4177 0.19 +1.9 81,978 + GFF4177 0.19 +0.8 81,978 + GFF4177 0.19 -1.2 81,979 - GFF4177 0.19 -0.2 81,979 - GFF4177 0.19 +0.2 81,979 - GFF4177 0.19 -0.3 82,065 + GFF4177 0.57 +1.8 82,066 - GFF4177 0.58 +0.4 82,066 - GFF4177 0.58 -1.2 82,206 - -0.7 82,284 - GFF4178 0.13 -0.4 82,292 + GFF4178 0.14 +0.4 82,292 + GFF4178 0.14 +0.3 82,701 + GFF4178 0.62 -0.9 82,701 + GFF4178 0.62 -1.2 82,701 + GFF4178 0.62 +1.1 82,701 + GFF4178 0.62 +0.2 82,702 - GFF4178 0.62 -0.4 82,702 - GFF4178 0.62 +1.6 82,785 + GFF4178 0.71 -0.1 82,786 - GFF4178 0.72 -0.3 82,796 + GFF4178 0.73 +0.2 82,844 + GFF4178 0.78 +0.6
Or see this region's nucleotide sequence