Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3766 and GFF3767 overlap by 14 nucleotides GFF3767 and GFF3768 overlap by 4 nucleotides GFF3768 and GFF3769 are separated by 17 nucleotides
GFF3766 - hypothetical protein, at 257,741 to 258,472
GFF3766
GFF3767 - 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases, at 258,459 to 259,235
GFF3767
GFF3768 - no description, at 259,232 to 260,482
GFF3768
GFF3769 - FIG022979: MoxR-like ATPases, at 260,500 to 261,495
GFF3769
Position (kb)
259
260
261 Strain fitness (log2 ratio)
-2
-1
0
1 at 258.347 kb on + strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.380 kb on + strand, within GFF3766 at 258.381 kb on - strand, within GFF3766 at 258.381 kb on - strand, within GFF3766 at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.964 kb on - strand, within GFF3767 at 259.119 kb on + strand, within GFF3767 at 259.119 kb on + strand, within GFF3767 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.367 kb on + strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.368 kb on - strand, within GFF3768 at 259.472 kb on + strand, within GFF3768 at 259.473 kb on - strand, within GFF3768 at 259.473 kb on - strand, within GFF3768 at 259.473 kb on - strand, within GFF3768 at 259.550 kb on + strand, within GFF3768 at 259.550 kb on + strand, within GFF3768 at 259.550 kb on + strand, within GFF3768 at 259.550 kb on + strand, within GFF3768 at 259.551 kb on - strand, within GFF3768 at 259.765 kb on + strand, within GFF3768 at 259.765 kb on + strand, within GFF3768 at 259.765 kb on + strand, within GFF3768 at 259.765 kb on + strand, within GFF3768 at 259.766 kb on - strand, within GFF3768 at 259.766 kb on - strand, within GFF3768 at 259.766 kb on - strand, within GFF3768 at 259.766 kb on - strand, within GFF3768 at 260.261 kb on + strand, within GFF3768 at 260.261 kb on + strand, within GFF3768 at 260.713 kb on + strand, within GFF3769 at 260.917 kb on + strand, within GFF3769 at 260.917 kb on + strand, within GFF3769 at 260.917 kb on + strand, within GFF3769 at 260.918 kb on - strand, within GFF3769 at 260.918 kb on - strand, within GFF3769 at 260.918 kb on - strand, within GFF3769 at 260.918 kb on - strand, within GFF3769 at 261.007 kb on + strand, within GFF3769 at 261.007 kb on + strand, within GFF3769 at 261.008 kb on - strand, within GFF3769 at 261.008 kb on - strand, within GFF3769 at 261.179 kb on + strand, within GFF3769 at 261.179 kb on + strand, within GFF3769 at 261.180 kb on - strand, within GFF3769 at 261.180 kb on - strand, within GFF3769 at 261.226 kb on + strand, within GFF3769 at 261.368 kb on + strand, within GFF3769 at 261.369 kb on - strand, within GFF3769
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 258,347 + GFF3766 0.83 -0.2 258,348 - GFF3766 0.83 -0.5 258,348 - GFF3766 0.83 +0.2 258,348 - GFF3766 0.83 -0.1 258,348 - GFF3766 0.83 -0.7 258,380 + GFF3766 0.87 +0.1 258,381 - GFF3766 0.87 -0.4 258,381 - GFF3766 0.87 -0.9 258,404 + +0.1 258,404 + -1.6 258,404 + -1.2 258,404 + +0.2 258,404 + +1.3 258,404 + +0.1 258,404 + -1.9 258,404 + -0.4 258,405 - -0.2 258,405 - +1.1 258,405 - -0.1 258,405 - +1.1 258,405 - -0.6 258,964 - GFF3767 0.65 -0.5 259,119 + GFF3767 0.85 +0.9 259,119 + GFF3767 0.85 -0.5 259,367 + GFF3768 0.11 -1.2 259,367 + GFF3768 0.11 -0.5 259,367 + GFF3768 0.11 +0.1 259,367 + GFF3768 0.11 -0.6 259,367 + GFF3768 0.11 +0.1 259,367 + GFF3768 0.11 -0.1 259,367 + GFF3768 0.11 -0.8 259,367 + GFF3768 0.11 +1.5 259,367 + GFF3768 0.11 +0.5 259,367 + GFF3768 0.11 +0.7 259,367 + GFF3768 0.11 +0.2 259,367 + GFF3768 0.11 +0.4 259,367 + GFF3768 0.11 +0.1 259,368 - GFF3768 0.11 +0.0 259,368 - GFF3768 0.11 -0.7 259,368 - GFF3768 0.11 -0.4 259,368 - GFF3768 0.11 +1.3 259,368 - GFF3768 0.11 +0.5 259,368 - GFF3768 0.11 -0.4 259,368 - GFF3768 0.11 -0.5 259,368 - GFF3768 0.11 -0.1 259,368 - GFF3768 0.11 +0.3 259,472 + GFF3768 0.19 +0.7 259,473 - GFF3768 0.19 +0.3 259,473 - GFF3768 0.19 -0.2 259,473 - GFF3768 0.19 -1.0 259,550 + GFF3768 0.25 -1.2 259,550 + GFF3768 0.25 +0.8 259,550 + GFF3768 0.25 -0.3 259,550 + GFF3768 0.25 +0.7 259,551 - GFF3768 0.25 -1.6 259,765 + GFF3768 0.43 -0.5 259,765 + GFF3768 0.43 -0.2 259,765 + GFF3768 0.43 +0.6 259,765 + GFF3768 0.43 +0.7 259,766 - GFF3768 0.43 +0.9 259,766 - GFF3768 0.43 +0.2 259,766 - GFF3768 0.43 -0.2 259,766 - GFF3768 0.43 -0.8 260,261 + GFF3768 0.82 -0.3 260,261 + GFF3768 0.82 +0.4 260,713 + GFF3769 0.21 +0.0 260,917 + GFF3769 0.42 -1.1 260,917 + GFF3769 0.42 +0.0 260,917 + GFF3769 0.42 -0.2 260,918 - GFF3769 0.42 -0.0 260,918 - GFF3769 0.42 +0.1 260,918 - GFF3769 0.42 +0.8 260,918 - GFF3769 0.42 +1.0 261,007 + GFF3769 0.51 +0.7 261,007 + GFF3769 0.51 +1.2 261,008 - GFF3769 0.51 -0.3 261,008 - GFF3769 0.51 +0.3 261,179 + GFF3769 0.68 -1.0 261,179 + GFF3769 0.68 -0.7 261,180 - GFF3769 0.68 -0.2 261,180 - GFF3769 0.68 +1.0 261,226 + GFF3769 0.73 -0.6 261,368 + GFF3769 0.87 -0.6 261,369 - GFF3769 0.87 +1.3
Or see this region's nucleotide sequence