Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3168 and GFF3169 are separated by 10 nucleotides GFF3169 and GFF3170 overlap by 4 nucleotides GFF3170 and GFF3171 are separated by 10 nucleotides
GFF3168 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 607,781 to 609,223
GFF3168
GFF3169 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 609,234 to 610,754
GFF3169
GFF3170 - hypothetical protein, at 610,751 to 611,548
GFF3170
GFF3171 - Uncharacterized protein conserved in bacteria, at 611,559 to 612,785
GFF3171
Position (kb) 609
610
611 Strain fitness (log2 ratio) -1
0
1 at 608.400 kb on - strand, within GFF3168 at 608.904 kb on - strand, within GFF3168 at 608.909 kb on + strand, within GFF3168 at 609.528 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.730 kb on - strand, within GFF3169 at 609.730 kb on - strand, within GFF3169 at 609.799 kb on - strand, within GFF3169 at 609.799 kb on - strand, within GFF3169 at 609.861 kb on + strand, within GFF3169 at 609.862 kb on - strand, within GFF3169 at 610.060 kb on + strand, within GFF3169 at 610.061 kb on - strand, within GFF3169 at 610.561 kb on - strand, within GFF3169 at 610.656 kb on + strand at 610.802 kb on + strand at 610.803 kb on - strand at 610.803 kb on - strand at 610.804 kb on + strand at 610.805 kb on - strand at 611.561 kb on - strand at 611.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 608,400 - GFF3168 0.43 -1.1 608,904 - GFF3168 0.78 +1.1 608,909 + GFF3168 0.78 +1.5 609,528 + GFF3169 0.19 -1.1 609,729 + GFF3169 0.33 +0.0 609,729 + GFF3169 0.33 -1.4 609,729 + GFF3169 0.33 +0.4 609,730 - GFF3169 0.33 -1.0 609,730 - GFF3169 0.33 -0.7 609,799 - GFF3169 0.37 -1.3 609,799 - GFF3169 0.37 -0.0 609,861 + GFF3169 0.41 -0.6 609,862 - GFF3169 0.41 -1.3 610,060 + GFF3169 0.54 -0.9 610,061 - GFF3169 0.54 -0.0 610,561 - GFF3169 0.87 -0.1 610,656 + +0.1 610,802 + -0.8 610,803 - -0.5 610,803 - -0.5 610,804 + -0.5 610,805 - -0.4 611,561 - +0.3 611,603 - -0.8
Or see this region's nucleotide sequence