Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3128 and GFF3129 are separated by 26 nucleotides GFF3129 and GFF3130 are separated by 24 nucleotides GFF3130 and GFF3131 are separated by 4 nucleotides GFF3131 and GFF3132 are separated by 41 nucleotides GFF3132 and GFF3133 are separated by 222 nucleotides
GFF3128 - T6SS PAAR-repeat protein / RhaS protein, at 554,159 to 558,616
GFF3128
GFF3129 - no description, at 558,643 to 559,086
GFF3129
GFF3130 - hypothetical protein, at 559,111 to 559,554
GFF3130
GFF3131 - VgrG protein, at 559,559 to 562,732
GFF3131
GFF3132 - T6SS component Hcp, at 562,774 to 563,256
GFF3132
GFF3133 - hypothetical protein, at 563,479 to 564,624
GFF3133
Position (kb) 559
560
561
562
563 Strain fitness (log2 ratio) -1
0
1
2 at 559.079 kb on + strand at 559.112 kb on + strand at 559.113 kb on - strand at 559.202 kb on + strand, within GFF3130 at 559.202 kb on + strand, within GFF3130 at 559.576 kb on - strand at 559.603 kb on - strand at 559.611 kb on + strand at 559.650 kb on + strand at 559.650 kb on + strand at 559.651 kb on - strand at 559.708 kb on - strand at 559.933 kb on - strand, within GFF3131 at 559.995 kb on + strand, within GFF3131 at 559.995 kb on + strand, within GFF3131 at 559.996 kb on - strand, within GFF3131 at 559.996 kb on - strand, within GFF3131 at 559.996 kb on - strand, within GFF3131 at 560.677 kb on + strand, within GFF3131 at 560.678 kb on - strand, within GFF3131 at 561.055 kb on - strand, within GFF3131 at 561.067 kb on - strand, within GFF3131 at 561.067 kb on - strand, within GFF3131 at 561.348 kb on + strand, within GFF3131 at 561.349 kb on - strand, within GFF3131 at 561.442 kb on - strand, within GFF3131 at 561.508 kb on - strand, within GFF3131 at 561.678 kb on + strand, within GFF3131 at 561.757 kb on - strand, within GFF3131 at 561.909 kb on + strand, within GFF3131 at 561.909 kb on + strand, within GFF3131 at 561.910 kb on - strand, within GFF3131 at 561.954 kb on + strand, within GFF3131 at 562.035 kb on + strand, within GFF3131 at 562.035 kb on + strand, within GFF3131 at 562.233 kb on + strand, within GFF3131 at 562.236 kb on + strand, within GFF3131 at 562.236 kb on + strand, within GFF3131 at 562.376 kb on + strand, within GFF3131 at 562.461 kb on + strand at 562.494 kb on + strand at 562.719 kb on + strand at 562.925 kb on + strand, within GFF3132 at 563.249 kb on - strand at 563.458 kb on + strand at 563.459 kb on - strand at 563.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 559,079 + -0.5 559,112 + +0.1 559,113 - -0.5 559,202 + GFF3130 0.20 -0.5 559,202 + GFF3130 0.20 -0.8 559,576 - +0.1 559,603 - -1.0 559,611 + +0.2 559,650 + +0.5 559,650 + -0.0 559,651 - +0.4 559,708 - +0.2 559,933 - GFF3131 0.12 -0.7 559,995 + GFF3131 0.14 +0.2 559,995 + GFF3131 0.14 +0.1 559,996 - GFF3131 0.14 +2.4 559,996 - GFF3131 0.14 +0.9 559,996 - GFF3131 0.14 -0.1 560,677 + GFF3131 0.35 -0.0 560,678 - GFF3131 0.35 -1.3 561,055 - GFF3131 0.47 -1.5 561,067 - GFF3131 0.48 +0.2 561,067 - GFF3131 0.48 -0.6 561,348 + GFF3131 0.56 -1.5 561,349 - GFF3131 0.56 -0.5 561,442 - GFF3131 0.59 -0.8 561,508 - GFF3131 0.61 -0.9 561,678 + GFF3131 0.67 +0.3 561,757 - GFF3131 0.69 +0.5 561,909 + GFF3131 0.74 +0.6 561,909 + GFF3131 0.74 +0.5 561,910 - GFF3131 0.74 -1.3 561,954 + GFF3131 0.75 +0.7 562,035 + GFF3131 0.78 +0.7 562,035 + GFF3131 0.78 -0.0 562,233 + GFF3131 0.84 +0.4 562,236 + GFF3131 0.84 +0.1 562,236 + GFF3131 0.84 +0.7 562,376 + GFF3131 0.89 +0.3 562,461 + -0.5 562,494 + +0.8 562,719 + +0.3 562,925 + GFF3132 0.31 -0.8 563,249 - +0.3 563,458 + +1.0 563,459 - -0.1 563,571 - -1.5
Or see this region's nucleotide sequence