Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2856 and GFF2857 are separated by 24 nucleotides GFF2857 and GFF2858 overlap by 4 nucleotides GFF2858 and GFF2859 are separated by 60 nucleotides
GFF2856 - Transcriptional regulator, LysR family, at 279,439 to 280,398
GFF2856
GFF2857 - Permease of the drug/metabolite transporter (DMT) superfamily, at 280,423 to 281,310
GFF2857
GFF2858 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 281,307 to 281,873
GFF2858
GFF2859 - Excinuclease ABC subunit C, at 281,934 to 283,901
GFF2859
Position (kb) 280
281
282 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 279.424 kb on - strand at 279.865 kb on + strand, within GFF2856 at 279.865 kb on + strand, within GFF2856 at 279.865 kb on + strand, within GFF2856 at 279.865 kb on + strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.866 kb on - strand, within GFF2856 at 279.970 kb on + strand, within GFF2856 at 279.970 kb on + strand, within GFF2856 at 279.971 kb on - strand, within GFF2856 at 279.971 kb on - strand, within GFF2856 at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.400 kb on + strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.401 kb on - strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.402 kb on + strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.403 kb on - strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.404 kb on + strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.405 kb on - strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.406 kb on + strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.407 kb on - strand at 280.550 kb on + strand, within GFF2857 at 280.550 kb on + strand, within GFF2857 at 280.550 kb on + strand, within GFF2857 at 280.551 kb on - strand, within GFF2857 at 280.551 kb on - strand, within GFF2857 at 280.551 kb on - strand, within GFF2857 at 280.551 kb on - strand, within GFF2857 at 280.588 kb on + strand, within GFF2857 at 280.588 kb on + strand, within GFF2857 at 280.588 kb on + strand, within GFF2857 at 280.588 kb on + strand, within GFF2857 at 280.588 kb on + strand, within GFF2857 at 280.589 kb on - strand, within GFF2857 at 280.589 kb on - strand, within GFF2857 at 280.589 kb on - strand, within GFF2857 at 280.589 kb on - strand, within GFF2857 at 280.589 kb on - strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.596 kb on + strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.597 kb on - strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.598 kb on + strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.599 kb on - strand, within GFF2857 at 280.800 kb on + strand, within GFF2857 at 281.051 kb on + strand, within GFF2857 at 281.168 kb on + strand, within GFF2857 at 281.169 kb on - strand, within GFF2857 at 281.169 kb on - strand, within GFF2857 at 281.201 kb on + strand, within GFF2857 at 281.201 kb on + strand, within GFF2857 at 281.201 kb on + strand, within GFF2857 at 281.202 kb on - strand, within GFF2857 at 281.315 kb on + strand at 281.316 kb on - strand at 281.316 kb on - strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.950 kb on + strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 281.951 kb on - strand at 282.083 kb on + strand at 282.083 kb on + strand at 282.211 kb on + strand, within GFF2859 at 282.211 kb on + strand, within GFF2859 at 282.211 kb on + strand, within GFF2859 at 282.212 kb on - strand, within GFF2859 at 282.212 kb on - strand, within GFF2859
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 279,424 - -0.1 279,865 + GFF2856 0.44 +0.9 279,865 + GFF2856 0.44 +0.8 279,865 + GFF2856 0.44 -1.2 279,865 + GFF2856 0.44 +0.2 279,866 - GFF2856 0.44 -0.2 279,866 - GFF2856 0.44 +0.9 279,866 - GFF2856 0.44 +1.2 279,866 - GFF2856 0.44 +0.0 279,866 - GFF2856 0.44 +0.5 279,866 - GFF2856 0.44 +0.9 279,866 - GFF2856 0.44 -0.8 279,866 - GFF2856 0.44 -0.2 279,970 + GFF2856 0.55 -0.9 279,970 + GFF2856 0.55 +0.3 279,971 - GFF2856 0.55 +0.8 279,971 - GFF2856 0.55 +1.1 280,400 + +0.3 280,400 + -0.2 280,400 + -0.0 280,400 + -0.2 280,400 + -1.7 280,400 + +0.5 280,400 + +2.2 280,400 + -0.4 280,400 + +0.2 280,400 + +0.9 280,400 + -0.1 280,401 - -0.2 280,401 - +1.5 280,401 - +0.0 280,401 - -0.3 280,401 - +0.2 280,401 - -0.4 280,401 - -0.2 280,401 - -0.1 280,402 + +0.7 280,402 + -0.2 280,402 + +1.5 280,402 + +0.1 280,402 + +0.2 280,402 + -0.4 280,402 + +1.0 280,402 + -0.0 280,402 + -0.2 280,402 + +0.9 280,402 + +0.3 280,402 + -1.1 280,402 + +1.4 280,402 + +0.5 280,403 - +0.3 280,403 - +0.4 280,403 - +1.2 280,403 - -0.5 280,403 - -0.3 280,403 - -0.6 280,403 - +0.0 280,403 - +0.5 280,403 - +1.5 280,403 - -0.3 280,403 - +0.6 280,403 - +1.7 280,404 + -1.8 280,404 + -0.1 280,404 + +0.6 280,404 + -0.1 280,404 + -0.1 280,404 + -0.1 280,404 + -0.5 280,404 + -0.3 280,404 + +0.3 280,404 + -0.4 280,404 + +0.9 280,404 + -1.1 280,404 + +0.1 280,404 + +2.1 280,404 + -1.0 280,404 + +0.1 280,404 + -0.4 280,404 + +1.3 280,404 + -0.1 280,404 + -0.5 280,404 + -0.7 280,404 + +0.8 280,404 + -0.2 280,405 - -0.4 280,405 - -0.8 280,405 - -0.6 280,405 - -0.3 280,405 - +0.7 280,405 - -0.1 280,405 - -0.9 280,405 - +0.2 280,405 - -0.1 280,405 - -0.1 280,405 - +0.5 280,405 - -0.2 280,405 - -0.1 280,405 - -1.7 280,405 - -0.9 280,405 - +0.4 280,405 - -0.6 280,405 - +0.9 280,405 - -0.2 280,405 - -1.7 280,405 - -0.5 280,405 - -0.0 280,405 - -1.1 280,406 + -0.1 280,406 + -0.8 280,406 + -0.3 280,406 + +0.7 280,406 + +0.5 280,406 + -2.3 280,406 + -2.1 280,406 + +0.2 280,406 + -1.0 280,406 + -0.7 280,406 + -0.5 280,406 + -0.4 280,406 + -0.3 280,406 + -0.4 280,406 + +0.9 280,406 + +0.7 280,406 + -0.6 280,406 + +0.8 280,406 + +0.1 280,406 + +0.8 280,406 + -0.7 280,406 + +0.1 280,406 + +0.2 280,406 + -0.0 280,406 + +0.8 280,407 - +0.3 280,407 - -0.4 280,407 - -0.8 280,407 - -0.1 280,407 - -0.3 280,407 - +1.5 280,407 - +0.3 280,407 - +0.3 280,407 - +0.2 280,407 - -0.3 280,407 - +0.5 280,407 - -0.1 280,407 - -0.0 280,407 - -0.2 280,407 - +3.1 280,407 - -2.1 280,407 - +1.5 280,407 - +0.4 280,550 + GFF2857 0.14 +0.3 280,550 + GFF2857 0.14 -0.6 280,550 + GFF2857 0.14 -0.5 280,551 - GFF2857 0.14 +0.1 280,551 - GFF2857 0.14 -0.3 280,551 - GFF2857 0.14 +0.4 280,551 - GFF2857 0.14 -0.5 280,588 + GFF2857 0.19 -0.6 280,588 + GFF2857 0.19 -0.1 280,588 + GFF2857 0.19 -0.6 280,588 + GFF2857 0.19 -0.3 280,588 + GFF2857 0.19 -0.2 280,589 - GFF2857 0.19 +0.2 280,589 - GFF2857 0.19 -0.8 280,589 - GFF2857 0.19 +1.5 280,589 - GFF2857 0.19 -0.7 280,589 - GFF2857 0.19 -0.8 280,596 + GFF2857 0.19 -0.5 280,596 + GFF2857 0.19 -0.3 280,596 + GFF2857 0.19 -0.4 280,596 + GFF2857 0.19 -0.5 280,596 + GFF2857 0.19 -0.9 280,596 + GFF2857 0.19 +0.1 280,596 + GFF2857 0.19 -0.5 280,596 + GFF2857 0.19 -0.2 280,596 + GFF2857 0.19 -1.9 280,596 + GFF2857 0.19 -0.4 280,596 + GFF2857 0.19 -0.1 280,596 + GFF2857 0.19 +0.0 280,596 + GFF2857 0.19 +0.2 280,596 + GFF2857 0.19 -0.4 280,596 + GFF2857 0.19 -1.2 280,596 + GFF2857 0.19 +0.0 280,596 + GFF2857 0.19 -0.8 280,596 + GFF2857 0.19 -0.6 280,596 + GFF2857 0.19 -0.3 280,597 - GFF2857 0.20 +0.3 280,597 - GFF2857 0.20 -1.1 280,597 - GFF2857 0.20 -0.7 280,597 - GFF2857 0.20 -0.8 280,597 - GFF2857 0.20 -0.2 280,597 - GFF2857 0.20 -0.4 280,597 - GFF2857 0.20 -0.2 280,597 - GFF2857 0.20 -0.5 280,597 - GFF2857 0.20 -0.9 280,597 - GFF2857 0.20 -1.3 280,597 - GFF2857 0.20 -0.5 280,597 - GFF2857 0.20 -0.7 280,597 - GFF2857 0.20 -0.3 280,597 - GFF2857 0.20 -1.8 280,597 - GFF2857 0.20 -0.6 280,597 - GFF2857 0.20 +0.6 280,597 - GFF2857 0.20 -0.0 280,597 - GFF2857 0.20 +0.1 280,597 - GFF2857 0.20 -0.5 280,597 - GFF2857 0.20 -1.3 280,597 - GFF2857 0.20 +0.1 280,597 - GFF2857 0.20 -0.3 280,597 - GFF2857 0.20 -0.6 280,598 + GFF2857 0.20 +2.5 280,598 + GFF2857 0.20 +0.8 280,598 + GFF2857 0.20 +0.4 280,598 + GFF2857 0.20 -0.2 280,598 + GFF2857 0.20 -0.7 280,598 + GFF2857 0.20 +0.3 280,598 + GFF2857 0.20 -0.2 280,598 + GFF2857 0.20 -0.1 280,598 + GFF2857 0.20 -0.5 280,598 + GFF2857 0.20 -0.2 280,598 + GFF2857 0.20 -0.3 280,598 + GFF2857 0.20 -0.2 280,598 + GFF2857 0.20 +0.2 280,599 - GFF2857 0.20 -0.1 280,599 - GFF2857 0.20 +0.1 280,599 - GFF2857 0.20 +0.7 280,599 - GFF2857 0.20 +0.1 280,599 - GFF2857 0.20 -0.3 280,599 - GFF2857 0.20 -0.4 280,599 - GFF2857 0.20 -0.1 280,599 - GFF2857 0.20 -0.4 280,599 - GFF2857 0.20 -0.2 280,599 - GFF2857 0.20 +1.4 280,599 - GFF2857 0.20 -0.5 280,599 - GFF2857 0.20 -0.3 280,599 - GFF2857 0.20 -0.7 280,599 - GFF2857 0.20 -0.5 280,599 - GFF2857 0.20 +0.9 280,599 - GFF2857 0.20 -0.2 280,599 - GFF2857 0.20 -0.3 280,599 - GFF2857 0.20 +0.6 280,599 - GFF2857 0.20 +0.5 280,800 + GFF2857 0.42 -0.4 281,051 + GFF2857 0.71 -0.5 281,168 + GFF2857 0.84 -0.0 281,169 - GFF2857 0.84 +1.2 281,169 - GFF2857 0.84 +0.1 281,201 + GFF2857 0.88 -0.8 281,201 + GFF2857 0.88 -1.1 281,201 + GFF2857 0.88 -1.8 281,202 - GFF2857 0.88 -0.8 281,315 + -0.5 281,316 - -1.1 281,316 - -0.7 281,950 + +0.1 281,950 + +0.1 281,950 + +0.3 281,950 + +1.4 281,950 + +0.3 281,950 + -0.6 281,950 + -0.2 281,950 + +0.4 281,951 - -0.5 281,951 - -0.8 281,951 - -0.9 281,951 - +0.9 281,951 - -0.4 281,951 - -0.6 281,951 - -0.2 281,951 - -0.3 281,951 - +0.6 281,951 - +0.9 281,951 - -0.8 281,951 - -0.2 281,951 - +1.1 281,951 - +0.7 281,951 - +1.0 282,083 + -0.4 282,083 + +0.5 282,211 + GFF2859 0.14 -0.1 282,211 + GFF2859 0.14 -0.9 282,211 + GFF2859 0.14 +0.1 282,212 - GFF2859 0.14 +0.3 282,212 - GFF2859 0.14 -0.2
Or see this region's nucleotide sequence