Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF281 and GFF282 are separated by 61 nucleotides GFF282 and GFF283 overlap by 4 nucleotides
GFF281 - Outer membrane porin protein 32 precursor, at 315,865 to 316,884
GFF281
GFF282 - hypothetical protein, at 316,946 to 318,133
GFF282
GFF283 - no description, at 318,130 to 319,224
GFF283
Position (kb)
316
317
318
319 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 316.207 kb on + strand, within GFF281 at 316.208 kb on - strand, within GFF281 at 316.348 kb on + strand, within GFF281 at 316.348 kb on + strand, within GFF281 at 316.349 kb on - strand, within GFF281 at 316.379 kb on - strand, within GFF281 at 316.426 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.448 kb on - strand, within GFF281 at 316.480 kb on + strand, within GFF281 at 316.480 kb on + strand, within GFF281 at 316.481 kb on - strand, within GFF281 at 316.481 kb on - strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.570 kb on + strand, within GFF281 at 316.570 kb on + strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.726 kb on + strand, within GFF281 at 316.726 kb on + strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.760 kb on + strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.801 kb on + strand at 316.801 kb on + strand at 316.801 kb on + strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.808 kb on + strand at 316.897 kb on - strand at 316.897 kb on - strand at 317.040 kb on + strand at 317.040 kb on + strand at 317.041 kb on - strand at 317.041 kb on - strand at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.221 kb on - strand, within GFF282 at 317.221 kb on - strand, within GFF282 at 317.316 kb on + strand, within GFF282 at 317.317 kb on - strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.425 kb on + strand, within GFF282 at 317.613 kb on + strand, within GFF282 at 317.613 kb on + strand, within GFF282 at 317.614 kb on - strand, within GFF282 at 317.614 kb on - strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.674 kb on - strand, within GFF282 at 317.697 kb on + strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.999 kb on - strand, within GFF282 at 317.999 kb on - strand, within GFF282 at 318.081 kb on + strand at 318.081 kb on + strand at 318.081 kb on + strand at 318.081 kb on + strand at 318.082 kb on - strand at 318.082 kb on - strand at 318.082 kb on - strand at 318.164 kb on + strand at 318.164 kb on + strand at 318.165 kb on - strand at 318.165 kb on - strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.225 kb on - strand at 318.225 kb on - strand at 318.225 kb on - strand at 318.318 kb on - strand, within GFF283 at 318.642 kb on - strand, within GFF283 at 318.686 kb on + strand, within GFF283 at 318.687 kb on - strand, within GFF283 at 318.932 kb on + strand, within GFF283 at 319.064 kb on + strand, within GFF283 at 319.064 kb on + strand, within GFF283 at 319.065 kb on - strand, within GFF283
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 316,207 + GFF281 0.34 +0.3 316,208 - GFF281 0.34 -0.4 316,348 + GFF281 0.47 -0.4 316,348 + GFF281 0.47 +0.4 316,349 - GFF281 0.47 +1.6 316,379 - GFF281 0.50 -0.0 316,426 + GFF281 0.55 -1.2 316,447 + GFF281 0.57 -0.2 316,447 + GFF281 0.57 -0.1 316,447 + GFF281 0.57 -0.2 316,447 + GFF281 0.57 +0.4 316,448 - GFF281 0.57 -0.4 316,480 + GFF281 0.60 +0.4 316,480 + GFF281 0.60 +0.3 316,481 - GFF281 0.60 -1.6 316,481 - GFF281 0.60 +0.2 316,537 + GFF281 0.66 +0.2 316,537 + GFF281 0.66 +1.5 316,537 + GFF281 0.66 -0.3 316,537 + GFF281 0.66 -0.4 316,538 - GFF281 0.66 -2.2 316,538 - GFF281 0.66 -2.3 316,538 - GFF281 0.66 -0.4 316,538 - GFF281 0.66 -0.6 316,561 + GFF281 0.68 -1.0 316,561 + GFF281 0.68 -1.6 316,561 + GFF281 0.68 +0.6 316,561 + GFF281 0.68 +0.6 316,561 + GFF281 0.68 -0.0 316,562 - GFF281 0.68 -0.4 316,562 - GFF281 0.68 +0.6 316,562 - GFF281 0.68 +2.2 316,562 - GFF281 0.68 -2.3 316,570 + GFF281 0.69 -1.2 316,570 + GFF281 0.69 -1.2 316,571 - GFF281 0.69 -1.2 316,571 - GFF281 0.69 -0.9 316,571 - GFF281 0.69 +0.1 316,726 + GFF281 0.84 +0.8 316,726 + GFF281 0.84 +0.8 316,727 - GFF281 0.85 +0.6 316,727 - GFF281 0.85 +0.3 316,727 - GFF281 0.85 -0.7 316,759 + GFF281 0.88 +0.2 316,759 + GFF281 0.88 -0.1 316,759 + GFF281 0.88 +1.2 316,760 + GFF281 0.88 +0.8 316,760 - GFF281 0.88 -0.2 316,760 - GFF281 0.88 -0.5 316,760 - GFF281 0.88 -0.6 316,801 + -2.5 316,801 + -0.7 316,801 + -0.5 316,802 - -0.6 316,802 - -0.4 316,802 - -0.2 316,802 - -0.7 316,802 - +0.6 316,808 + -0.6 316,897 - -0.2 316,897 - -1.8 317,040 + -0.5 317,040 + -0.7 317,041 - -1.2 317,041 - -0.4 317,220 + GFF282 0.23 -0.0 317,220 + GFF282 0.23 -0.5 317,220 + GFF282 0.23 -0.4 317,220 + GFF282 0.23 -2.2 317,220 + GFF282 0.23 -0.6 317,220 + GFF282 0.23 +0.5 317,220 + GFF282 0.23 +0.1 317,220 + GFF282 0.23 +0.1 317,220 + GFF282 0.23 +0.3 317,220 + GFF282 0.23 -2.1 317,221 - GFF282 0.23 +1.8 317,221 - GFF282 0.23 +0.2 317,316 + GFF282 0.31 -1.1 317,317 - GFF282 0.31 +0.5 317,352 + GFF282 0.34 +0.3 317,352 + GFF282 0.34 -1.2 317,352 + GFF282 0.34 -0.2 317,352 + GFF282 0.34 +1.0 317,352 + GFF282 0.34 -0.1 317,352 + GFF282 0.34 +0.4 317,353 - GFF282 0.34 -0.6 317,353 - GFF282 0.34 -0.0 317,353 - GFF282 0.34 +1.6 317,425 + GFF282 0.40 +0.4 317,613 + GFF282 0.56 +0.3 317,613 + GFF282 0.56 -1.6 317,614 - GFF282 0.56 -0.0 317,614 - GFF282 0.56 -2.1 317,673 + GFF282 0.61 -0.4 317,673 + GFF282 0.61 +0.6 317,673 + GFF282 0.61 +1.2 317,674 - GFF282 0.61 +0.8 317,697 + GFF282 0.63 -1.3 317,698 - GFF282 0.63 -0.4 317,698 - GFF282 0.63 +0.1 317,698 - GFF282 0.63 -0.1 317,698 - GFF282 0.63 +0.6 317,999 - GFF282 0.89 +1.0 317,999 - GFF282 0.89 +0.4 318,081 + -0.3 318,081 + -0.5 318,081 + +1.0 318,081 + -0.7 318,082 - +0.1 318,082 - +0.2 318,082 - -0.8 318,164 + +0.4 318,164 + -0.4 318,165 - -1.6 318,165 - -0.4 318,224 + +0.2 318,224 + -0.6 318,224 + +1.2 318,224 + -0.2 318,224 + -0.2 318,225 - -0.3 318,225 - +0.0 318,225 - -0.3 318,318 - GFF283 0.17 -0.2 318,642 - GFF283 0.47 +0.4 318,686 + GFF283 0.51 -0.2 318,687 - GFF283 0.51 -1.4 318,932 + GFF283 0.73 -1.6 319,064 + GFF283 0.85 +0.3 319,064 + GFF283 0.85 -0.1 319,065 - GFF283 0.85 -0.1
Or see this region's nucleotide sequence