Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2715 and GFF2716 are separated by 5 nucleotides GFF2716 and GFF2717 are separated by 19 nucleotides GFF2717 and GFF2718 overlap by 1 nucleotides
GFF2715 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 148,799 to 149,782
GFF2715
GFF2716 - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45), at 149,788 to 150,741
GFF2716
GFF2717 - Epimerase KguE, at 150,761 to 151,510
GFF2717
GFF2718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 151,510 to 152,265
GFF2718
Position (kb) 149
150
151 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 148.818 kb on + strand at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.263 kb on - strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.545 kb on - strand, within GFF2715 at 150.023 kb on + strand, within GFF2716 at 150.023 kb on + strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.026 kb on + strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.729 kb on + strand at 150.729 kb on + strand at 150.729 kb on + strand at 150.762 kb on + strand at 150.763 kb on - strand at 150.763 kb on - strand at 150.785 kb on + strand at 150.785 kb on + strand at 150.786 kb on - strand at 150.966 kb on + strand, within GFF2717 at 150.966 kb on + strand, within GFF2717 at 150.966 kb on + strand, within GFF2717 at 151.329 kb on + strand, within GFF2717 at 151.329 kb on + strand, within GFF2717 at 151.330 kb on - strand, within GFF2717
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 148,818 + -0.3 149,262 + GFF2715 0.47 -0.5 149,262 + GFF2715 0.47 -0.4 149,262 + GFF2715 0.47 -0.5 149,263 - GFF2715 0.47 +0.4 149,544 + GFF2715 0.76 +0.8 149,544 + GFF2715 0.76 -0.6 149,544 + GFF2715 0.76 -0.1 149,545 - GFF2715 0.76 +0.5 150,023 + GFF2716 0.25 +0.6 150,023 + GFF2716 0.25 +1.2 150,024 - GFF2716 0.25 +0.5 150,024 - GFF2716 0.25 -2.0 150,024 - GFF2716 0.25 -0.7 150,026 + GFF2716 0.25 +0.1 150,414 - GFF2716 0.66 +0.9 150,414 - GFF2716 0.66 +0.1 150,729 + +2.2 150,729 + -0.3 150,729 + +1.5 150,762 + +0.6 150,763 - +0.6 150,763 - +3.1 150,785 + -0.4 150,785 + -1.2 150,786 - -0.1 150,966 + GFF2717 0.27 +0.3 150,966 + GFF2717 0.27 -0.3 150,966 + GFF2717 0.27 -0.5 151,329 + GFF2717 0.76 +0.1 151,329 + GFF2717 0.76 -0.3 151,330 - GFF2717 0.76 -0.9
Or see this region's nucleotide sequence