Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2665 and GFF2666 are separated by 71 nucleotides GFF2666 and GFF2667 are separated by 5 nucleotides GFF2667 and GFF2668 are separated by 9 nucleotides
GFF2665 - FIG001454: Transglutaminase-like enzymes, putative cysteine proteases, at 87,493 to 89,514
GFF2665
GFF2666 - Membrane-bound lytic murein transglycosylase B, at 89,586 to 90,668
GFF2666
GFF2667 - COG4339 metal-dependent phosphohydrolase, HD superfamily, at 90,674 to 91,294
GFF2667
GFF2668 - hypothetical protein, at 91,304 to 91,792
GFF2668
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1 at 88.688 kb on - strand, within GFF2665 at 88.693 kb on + strand, within GFF2665 at 88.693 kb on + strand, within GFF2665 at 88.694 kb on - strand, within GFF2665 at 88.694 kb on - strand, within GFF2665 at 88.694 kb on - strand, within GFF2665 at 88.871 kb on - strand, within GFF2665 at 88.871 kb on - strand, within GFF2665 at 88.871 kb on - strand, within GFF2665 at 88.871 kb on - strand, within GFF2665 at 89.113 kb on + strand, within GFF2665 at 89.113 kb on + strand, within GFF2665 at 89.113 kb on + strand, within GFF2665 at 89.114 kb on - strand, within GFF2665 at 89.186 kb on - strand, within GFF2665 at 89.186 kb on - strand, within GFF2665 at 89.186 kb on - strand, within GFF2665 at 89.186 kb on - strand, within GFF2665 at 89.197 kb on + strand, within GFF2665 at 89.198 kb on - strand, within GFF2665 at 89.429 kb on - strand at 89.653 kb on + strand at 89.654 kb on - strand at 89.654 kb on - strand at 89.654 kb on - strand at 89.654 kb on - strand at 89.716 kb on - strand, within GFF2666 at 89.881 kb on - strand, within GFF2666 at 89.973 kb on + strand, within GFF2666 at 89.974 kb on - strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.024 kb on + strand, within GFF2666 at 90.025 kb on - strand, within GFF2666 at 90.025 kb on - strand, within GFF2666 at 90.025 kb on - strand, within GFF2666 at 90.202 kb on - strand, within GFF2666 at 90.202 kb on - strand, within GFF2666 at 90.252 kb on + strand, within GFF2666 at 90.252 kb on + strand, within GFF2666 at 90.330 kb on + strand, within GFF2666 at 90.330 kb on + strand, within GFF2666 at 90.330 kb on + strand, within GFF2666 at 90.373 kb on - strand, within GFF2666 at 90.373 kb on - strand, within GFF2666 at 90.555 kb on + strand, within GFF2666 at 90.555 kb on + strand, within GFF2666 at 90.555 kb on + strand, within GFF2666 at 90.555 kb on + strand, within GFF2666 at 90.556 kb on - strand, within GFF2666 at 90.556 kb on - strand, within GFF2666 at 90.579 kb on + strand at 90.609 kb on + strand at 90.609 kb on + strand at 90.610 kb on - strand at 90.610 kb on - strand at 90.610 kb on - strand at 90.610 kb on - strand at 90.774 kb on - strand, within GFF2667 at 90.794 kb on + strand, within GFF2667 at 90.905 kb on + strand, within GFF2667 at 91.118 kb on + strand, within GFF2667 at 91.119 kb on - strand, within GFF2667 at 91.220 kb on + strand, within GFF2667 at 91.221 kb on - strand, within GFF2667 at 91.221 kb on - strand, within GFF2667 at 91.665 kb on + strand, within GFF2668 at 91.665 kb on + strand, within GFF2668 at 91.666 kb on - strand, within GFF2668 at 91.666 kb on - strand, within GFF2668
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 88,688 - GFF2665 0.59 -0.5 88,693 + GFF2665 0.59 -0.2 88,693 + GFF2665 0.59 -0.3 88,694 - GFF2665 0.59 +0.2 88,694 - GFF2665 0.59 +0.5 88,694 - GFF2665 0.59 -1.4 88,871 - GFF2665 0.68 -0.6 88,871 - GFF2665 0.68 +0.2 88,871 - GFF2665 0.68 +0.2 88,871 - GFF2665 0.68 -0.5 89,113 + GFF2665 0.80 -0.3 89,113 + GFF2665 0.80 -0.3 89,113 + GFF2665 0.80 -1.0 89,114 - GFF2665 0.80 -0.5 89,186 - GFF2665 0.84 +0.7 89,186 - GFF2665 0.84 -0.3 89,186 - GFF2665 0.84 -0.2 89,186 - GFF2665 0.84 -1.1 89,197 + GFF2665 0.84 +0.4 89,198 - GFF2665 0.84 +0.7 89,429 - -0.5 89,653 + -1.2 89,654 - +0.4 89,654 - -0.8 89,654 - -0.6 89,654 - +0.1 89,716 - GFF2666 0.12 -1.3 89,881 - GFF2666 0.27 -2.7 89,973 + GFF2666 0.36 -0.3 89,974 - GFF2666 0.36 +0.9 90,024 + GFF2666 0.40 -0.4 90,024 + GFF2666 0.40 -1.1 90,024 + GFF2666 0.40 -0.8 90,024 + GFF2666 0.40 +0.5 90,024 + GFF2666 0.40 +0.2 90,024 + GFF2666 0.40 -0.4 90,024 + GFF2666 0.40 -0.8 90,024 + GFF2666 0.40 -0.1 90,024 + GFF2666 0.40 -0.7 90,025 - GFF2666 0.41 -0.1 90,025 - GFF2666 0.41 -0.8 90,025 - GFF2666 0.41 +0.1 90,202 - GFF2666 0.57 -0.8 90,202 - GFF2666 0.57 +0.0 90,252 + GFF2666 0.61 -0.1 90,252 + GFF2666 0.61 -0.1 90,330 + GFF2666 0.69 -0.3 90,330 + GFF2666 0.69 -0.5 90,330 + GFF2666 0.69 -0.1 90,373 - GFF2666 0.73 -0.9 90,373 - GFF2666 0.73 -0.6 90,555 + GFF2666 0.89 -0.4 90,555 + GFF2666 0.89 +0.9 90,555 + GFF2666 0.89 -0.3 90,555 + GFF2666 0.89 -0.9 90,556 - GFF2666 0.90 -0.8 90,556 - GFF2666 0.90 -0.2 90,579 + -1.7 90,609 + -1.1 90,609 + +0.6 90,610 - +1.2 90,610 - +0.3 90,610 - -0.8 90,610 - -0.5 90,774 - GFF2667 0.16 -0.9 90,794 + GFF2667 0.19 -0.2 90,905 + GFF2667 0.37 +0.3 91,118 + GFF2667 0.71 +0.5 91,119 - GFF2667 0.72 +0.2 91,220 + GFF2667 0.88 +0.0 91,221 - GFF2667 0.88 -1.2 91,221 - GFF2667 0.88 +0.5 91,665 + GFF2668 0.74 -0.3 91,665 + GFF2668 0.74 -0.5 91,666 - GFF2668 0.74 -0.5 91,666 - GFF2668 0.74 -2.1
Or see this region's nucleotide sequence