Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2661 and GFF2662 are separated by 39 nucleotides GFF2662 and GFF2663 are separated by 73 nucleotides GFF2663 and GFF2664 overlap by 4 nucleotides
GFF2661 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 83,233 to 84,513
GFF2661
GFF2662 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 84,553 to 85,506
GFF2662
GFF2663 - FIG022979: MoxR-like ATPases, at 85,580 to 86,500
GFF2663
GFF2664 - FIG002343: hypothetical protein, at 86,497 to 87,474
GFF2664
Position (kb) 84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 83.667 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.974 kb on - strand, within GFF2661 at 84.554 kb on + strand at 84.554 kb on + strand at 84.554 kb on + strand at 84.555 kb on - strand at 84.615 kb on - strand at 84.845 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.900 kb on - strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 85.490 kb on + strand at 85.490 kb on + strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.519 kb on + strand at 85.519 kb on + strand at 85.520 kb on - strand at 85.520 kb on - strand at 85.551 kb on + strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.552 kb on - strand at 85.573 kb on + strand at 85.574 kb on - strand at 85.574 kb on - strand at 85.574 kb on - strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.579 kb on + strand at 85.580 kb on - strand at 85.586 kb on + strand at 85.599 kb on + strand at 85.599 kb on + strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.611 kb on + strand at 85.612 kb on - strand at 85.621 kb on + strand at 85.621 kb on + strand at 85.621 kb on + strand at 85.622 kb on - strand at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.826 kb on + strand, within GFF2663 at 85.827 kb on - strand, within GFF2663 at 85.827 kb on - strand, within GFF2663 at 86.246 kb on + strand, within GFF2663 at 86.246 kb on + strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.247 kb on - strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.369 kb on + strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663 at 86.370 kb on - strand, within GFF2663
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 83,667 - GFF2661 0.34 +2.5 83,769 - GFF2661 0.42 +0.7 83,769 - GFF2661 0.42 +1.2 83,769 - GFF2661 0.42 +1.5 83,974 - GFF2661 0.58 +0.4 84,554 + -0.6 84,554 + -0.4 84,554 + -2.2 84,555 - +0.1 84,615 - -0.0 84,845 + GFF2662 0.31 +0.0 84,899 + GFF2662 0.36 +0.1 84,899 + GFF2662 0.36 -1.3 84,900 - GFF2662 0.36 +2.5 84,905 + GFF2662 0.37 -0.6 84,905 + GFF2662 0.37 -0.4 84,906 - GFF2662 0.37 +0.3 84,906 - GFF2662 0.37 -0.3 84,906 - GFF2662 0.37 -0.7 84,906 - GFF2662 0.37 -1.0 84,906 - GFF2662 0.37 +0.8 85,490 + -0.2 85,490 + -0.4 85,491 - -1.4 85,491 - +0.5 85,491 - +0.4 85,491 - -0.2 85,491 - -0.4 85,519 + -0.1 85,519 + -3.3 85,520 - -0.4 85,520 - +0.4 85,551 + +0.5 85,552 - -1.0 85,552 - -0.2 85,552 - -0.8 85,552 - -2.2 85,552 - +0.3 85,573 + +0.2 85,574 - +1.0 85,574 - -0.6 85,574 - +0.0 85,579 + +0.1 85,579 + -1.4 85,579 + -1.0 85,579 + +0.1 85,580 - -0.9 85,586 + -0.8 85,599 + +0.5 85,599 + +0.0 85,600 - -0.6 85,600 - +0.1 85,611 + +0.0 85,612 - -1.1 85,621 + -0.5 85,621 + -1.9 85,621 + +0.7 85,622 - +0.0 85,826 + GFF2663 0.27 +0.5 85,826 + GFF2663 0.27 -3.2 85,826 + GFF2663 0.27 -0.2 85,826 + GFF2663 0.27 -0.1 85,826 + GFF2663 0.27 +0.3 85,827 - GFF2663 0.27 -0.2 85,827 - GFF2663 0.27 -0.0 86,246 + GFF2663 0.72 +0.7 86,246 + GFF2663 0.72 -0.4 86,247 - GFF2663 0.72 +0.4 86,247 - GFF2663 0.72 -0.2 86,247 - GFF2663 0.72 -0.5 86,247 - GFF2663 0.72 +0.3 86,247 - GFF2663 0.72 -1.1 86,369 + GFF2663 0.86 -0.2 86,369 + GFF2663 0.86 -1.2 86,369 + GFF2663 0.86 -0.4 86,370 - GFF2663 0.86 -1.1 86,370 - GFF2663 0.86 +0.4 86,370 - GFF2663 0.86 +1.1 86,370 - GFF2663 0.86 +0.0
Or see this region's nucleotide sequence