Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF245 and GFF246 are separated by 131 nucleotides GFF246 and GFF247 are separated by 72 nucleotides GFF247 and GFF248 are separated by 274 nucleotides
GFF245 - Aspartate aminotransferase (EC 2.6.1.1), at 272,796 to 273,968
GFF245
GFF246 - Transmembrane protein Msl3831, at 274,100 to 274,258
GFF246
GFF247 - ATP-dependent helicase HrpA, at 274,331 to 278,116
GFF247
GFF248 - Cell division protein FtsH (EC 3.4.24.-), at 278,391 to 280,544
GFF248
Position (kb) 274
275
276
277
278
279 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 273.420 kb on + strand, within GFF245 at 273.420 kb on + strand, within GFF245 at 273.421 kb on - strand, within GFF245 at 273.421 kb on - strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.519 kb on + strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.520 kb on - strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.645 kb on + strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.646 kb on - strand, within GFF245 at 273.666 kb on + strand, within GFF245 at 273.687 kb on + strand, within GFF245 at 273.688 kb on - strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.765 kb on + strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.766 kb on - strand, within GFF245 at 273.873 kb on + strand at 273.873 kb on + strand at 273.873 kb on + strand at 273.874 kb on - strand at 273.874 kb on - strand at 273.874 kb on - strand at 273.874 kb on - strand at 274.000 kb on + strand at 274.140 kb on + strand, within GFF246 at 274.140 kb on + strand, within GFF246 at 274.141 kb on - strand, within GFF246 at 274.141 kb on - strand, within GFF246 at 274.147 kb on - strand, within GFF246 at 274.169 kb on + strand, within GFF246 at 274.169 kb on + strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.170 kb on - strand, within GFF246 at 274.171 kb on + strand, within GFF246 at 274.172 kb on - strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.187 kb on + strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.188 kb on - strand, within GFF246 at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.254 kb on + strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.255 kb on - strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.256 kb on + strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.257 kb on - strand at 274.497 kb on + strand at 274.498 kb on - strand at 274.735 kb on - strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.923 kb on + strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.924 kb on - strand, within GFF247 at 274.969 kb on - strand, within GFF247 at 274.969 kb on - strand, within GFF247 at 274.969 kb on - strand, within GFF247 at 275.131 kb on - strand, within GFF247 at 275.739 kb on + strand, within GFF247 at 275.739 kb on + strand, within GFF247 at 275.739 kb on + strand, within GFF247 at 275.739 kb on + strand, within GFF247 at 275.739 kb on + strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.740 kb on - strand, within GFF247 at 275.841 kb on + strand, within GFF247 at 275.841 kb on + strand, within GFF247 at 275.842 kb on - strand, within GFF247 at 275.842 kb on - strand, within GFF247 at 275.877 kb on + strand, within GFF247 at 275.931 kb on + strand, within GFF247 at 275.932 kb on - strand, within GFF247 at 275.932 kb on - strand, within GFF247 at 276.138 kb on + strand, within GFF247 at 276.138 kb on + strand, within GFF247 at 276.138 kb on + strand, within GFF247 at 276.139 kb on - strand, within GFF247 at 276.139 kb on - strand, within GFF247 at 276.153 kb on + strand, within GFF247 at 276.154 kb on - strand, within GFF247 at 276.154 kb on - strand, within GFF247 at 276.154 kb on - strand, within GFF247 at 276.369 kb on + strand, within GFF247 at 276.369 kb on + strand, within GFF247 at 276.369 kb on + strand, within GFF247 at 276.369 kb on + strand, within GFF247 at 276.369 kb on + strand, within GFF247 at 276.370 kb on + strand, within GFF247 at 276.370 kb on - strand, within GFF247 at 276.392 kb on + strand, within GFF247 at 276.392 kb on + strand, within GFF247 at 276.392 kb on + strand, within GFF247 at 276.393 kb on - strand, within GFF247 at 276.393 kb on - strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.396 kb on + strand, within GFF247 at 276.397 kb on - strand, within GFF247 at 276.397 kb on - strand, within GFF247 at 276.397 kb on - strand, within GFF247 at 276.397 kb on - strand, within GFF247 at 276.397 kb on - strand, within GFF247 at 276.597 kb on + strand, within GFF247 at 276.597 kb on + strand, within GFF247 at 276.598 kb on - strand, within GFF247 at 276.622 kb on + strand, within GFF247 at 276.933 kb on + strand, within GFF247 at 276.934 kb on - strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.095 kb on + strand, within GFF247 at 277.245 kb on + strand, within GFF247 at 277.245 kb on + strand, within GFF247 at 277.246 kb on - strand, within GFF247 at 277.564 kb on - strand, within GFF247 at 277.719 kb on + strand, within GFF247 at 277.719 kb on + strand, within GFF247 at 277.720 kb on - strand, within GFF247 at 277.782 kb on + strand at 277.782 kb on + strand at 277.782 kb on + strand at 277.783 kb on - strand at 277.783 kb on - strand at 277.783 kb on - strand at 277.783 kb on - strand at 278.232 kb on + strand at 278.298 kb on - strand at 278.301 kb on + strand at 278.302 kb on - strand at 278.309 kb on - strand at 278.329 kb on + strand at 278.329 kb on + strand at 278.329 kb on + strand at 278.330 kb on - strand at 278.330 kb on - strand at 278.348 kb on + strand at 278.462 kb on + strand at 278.514 kb on + strand at 278.515 kb on - strand at 278.515 kb on - strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.520 kb on + strand at 278.521 kb on - strand at 278.521 kb on - strand at 278.521 kb on - strand at 278.521 kb on - strand at 278.691 kb on + strand, within GFF248 at 278.691 kb on + strand, within GFF248 at 278.691 kb on + strand, within GFF248 at 278.692 kb on - strand, within GFF248 at 278.692 kb on - strand, within GFF248 at 278.799 kb on + strand, within GFF248 at 278.800 kb on - strand, within GFF248 at 278.800 kb on - strand, within GFF248 at 278.800 kb on - strand, within GFF248 at 278.800 kb on - strand, within GFF248 at 278.800 kb on - strand, within GFF248 at 278.850 kb on + strand, within GFF248 at 279.081 kb on + strand, within GFF248 at 279.081 kb on + strand, within GFF248 at 279.082 kb on + strand, within GFF248 at 279.082 kb on - strand, within GFF248
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 273,420 + GFF245 0.53 +0.1 273,420 + GFF245 0.53 +0.9 273,421 - GFF245 0.53 -0.4 273,421 - GFF245 0.53 -0.1 273,519 + GFF245 0.62 +0.4 273,519 + GFF245 0.62 -1.1 273,519 + GFF245 0.62 +0.8 273,519 + GFF245 0.62 -0.0 273,519 + GFF245 0.62 -0.1 273,520 - GFF245 0.62 +0.2 273,520 - GFF245 0.62 +1.0 273,520 - GFF245 0.62 -0.5 273,520 - GFF245 0.62 -0.3 273,520 - GFF245 0.62 -0.6 273,645 + GFF245 0.72 +0.8 273,645 + GFF245 0.72 +0.0 273,645 + GFF245 0.72 +2.2 273,646 - GFF245 0.72 +0.6 273,646 - GFF245 0.72 -0.9 273,646 - GFF245 0.72 +0.4 273,646 - GFF245 0.72 +0.9 273,666 + GFF245 0.74 +0.0 273,687 + GFF245 0.76 +1.4 273,688 - GFF245 0.76 -1.4 273,765 + GFF245 0.83 +0.5 273,765 + GFF245 0.83 -0.0 273,765 + GFF245 0.83 -0.6 273,765 + GFF245 0.83 +0.4 273,766 - GFF245 0.83 -0.7 273,766 - GFF245 0.83 +0.2 273,766 - GFF245 0.83 -0.5 273,766 - GFF245 0.83 -0.6 273,766 - GFF245 0.83 -0.8 273,873 + -0.2 273,873 + -0.1 273,873 + -0.5 273,874 - -0.2 273,874 - +0.2 273,874 - +0.7 273,874 - -1.8 274,000 + +1.9 274,140 + GFF246 0.25 -0.0 274,140 + GFF246 0.25 +0.2 274,141 - GFF246 0.26 +0.7 274,141 - GFF246 0.26 -0.6 274,147 - GFF246 0.30 -1.3 274,169 + GFF246 0.43 +0.3 274,169 + GFF246 0.43 +1.2 274,170 - GFF246 0.44 -0.1 274,170 - GFF246 0.44 -0.5 274,170 - GFF246 0.44 -0.1 274,171 + GFF246 0.45 +0.3 274,172 - GFF246 0.45 -0.4 274,187 + GFF246 0.55 -0.9 274,187 + GFF246 0.55 +0.2 274,187 + GFF246 0.55 -0.6 274,188 - GFF246 0.55 -1.0 274,188 - GFF246 0.55 +0.1 274,188 - GFF246 0.55 -0.1 274,188 - GFF246 0.55 +0.2 274,188 - GFF246 0.55 -2.6 274,254 + -0.5 274,254 + -0.8 274,254 + +1.5 274,254 + +1.0 274,254 + +0.7 274,254 + +0.1 274,254 + -0.0 274,254 + +0.1 274,254 + -1.6 274,254 + +0.6 274,254 + -0.3 274,255 - -0.4 274,255 - +0.9 274,255 - -0.1 274,255 - -0.4 274,255 - -2.1 274,255 - -0.1 274,255 - -0.7 274,255 - -0.0 274,255 - +0.7 274,256 + -1.6 274,256 + +0.1 274,256 + +0.9 274,256 + +0.5 274,256 + -0.3 274,256 + -0.0 274,256 + +0.2 274,256 + +0.7 274,256 + +0.7 274,256 + -0.4 274,256 + +1.0 274,257 - +0.7 274,257 - -0.0 274,257 - -1.8 274,257 - -0.5 274,257 - -0.0 274,257 - -0.9 274,257 - -0.7 274,257 - -0.9 274,257 - -0.5 274,257 - -0.2 274,497 + -0.6 274,498 - -0.7 274,735 - GFF247 0.11 +0.9 274,923 + GFF247 0.16 -1.1 274,923 + GFF247 0.16 +0.0 274,923 + GFF247 0.16 -0.4 274,923 + GFF247 0.16 -0.2 274,924 - GFF247 0.16 -0.2 274,924 - GFF247 0.16 +1.2 274,924 - GFF247 0.16 +0.0 274,924 - GFF247 0.16 -0.3 274,924 - GFF247 0.16 +0.1 274,924 - GFF247 0.16 +0.2 274,969 - GFF247 0.17 +0.9 274,969 - GFF247 0.17 -0.9 274,969 - GFF247 0.17 -1.1 275,131 - GFF247 0.21 +0.8 275,739 + GFF247 0.37 -1.9 275,739 + GFF247 0.37 -0.4 275,739 + GFF247 0.37 -2.3 275,739 + GFF247 0.37 -0.1 275,739 + GFF247 0.37 +1.5 275,740 - GFF247 0.37 -0.7 275,740 - GFF247 0.37 -1.1 275,740 - GFF247 0.37 +0.5 275,740 - GFF247 0.37 -0.4 275,740 - GFF247 0.37 +0.2 275,740 - GFF247 0.37 -0.2 275,740 - GFF247 0.37 +0.2 275,740 - GFF247 0.37 -0.0 275,740 - GFF247 0.37 -1.1 275,740 - GFF247 0.37 -0.1 275,841 + GFF247 0.40 -0.6 275,841 + GFF247 0.40 +3.5 275,842 - GFF247 0.40 -1.3 275,842 - GFF247 0.40 -1.8 275,877 + GFF247 0.41 -1.1 275,931 + GFF247 0.42 -0.2 275,932 - GFF247 0.42 +0.4 275,932 - GFF247 0.42 -0.2 276,138 + GFF247 0.48 -1.8 276,138 + GFF247 0.48 +0.4 276,138 + GFF247 0.48 -0.2 276,139 - GFF247 0.48 -0.3 276,139 - GFF247 0.48 -0.2 276,153 + GFF247 0.48 -0.3 276,154 - GFF247 0.48 -0.9 276,154 - GFF247 0.48 +2.9 276,154 - GFF247 0.48 +0.1 276,369 + GFF247 0.54 -0.6 276,369 + GFF247 0.54 -0.1 276,369 + GFF247 0.54 -0.2 276,369 + GFF247 0.54 +0.4 276,369 + GFF247 0.54 +0.6 276,370 + GFF247 0.54 -0.1 276,370 - GFF247 0.54 -0.4 276,392 + GFF247 0.54 +0.4 276,392 + GFF247 0.54 -2.9 276,392 + GFF247 0.54 -0.4 276,393 - GFF247 0.54 -0.5 276,393 - GFF247 0.54 +0.8 276,396 + GFF247 0.55 -1.2 276,396 + GFF247 0.55 +1.2 276,396 + GFF247 0.55 +0.4 276,396 + GFF247 0.55 -1.5 276,396 + GFF247 0.55 -0.3 276,396 + GFF247 0.55 +0.0 276,397 - GFF247 0.55 -1.1 276,397 - GFF247 0.55 +0.9 276,397 - GFF247 0.55 -0.8 276,397 - GFF247 0.55 -0.8 276,397 - GFF247 0.55 +0.2 276,597 + GFF247 0.60 -1.6 276,597 + GFF247 0.60 -1.4 276,598 - GFF247 0.60 -0.2 276,622 + GFF247 0.61 -0.1 276,933 + GFF247 0.69 +1.9 276,934 - GFF247 0.69 -1.3 277,095 + GFF247 0.73 -0.1 277,095 + GFF247 0.73 -0.4 277,095 + GFF247 0.73 -0.2 277,095 + GFF247 0.73 -0.8 277,095 + GFF247 0.73 -3.4 277,095 + GFF247 0.73 +0.4 277,245 + GFF247 0.77 -1.0 277,245 + GFF247 0.77 -1.0 277,246 - GFF247 0.77 -1.5 277,564 - GFF247 0.85 -1.0 277,719 + GFF247 0.89 -0.3 277,719 + GFF247 0.89 +0.8 277,720 - GFF247 0.90 +1.5 277,782 + -0.4 277,782 + -0.4 277,782 + -0.7 277,783 - -0.7 277,783 - +0.8 277,783 - +0.3 277,783 - -2.1 278,232 + +0.8 278,298 - -0.6 278,301 + +0.9 278,302 - -0.3 278,309 - -1.0 278,329 + +0.6 278,329 + +0.0 278,329 + -0.0 278,330 - -0.2 278,330 - +0.3 278,348 + +0.7 278,462 + -1.0 278,514 + -0.3 278,515 - +1.8 278,515 - +1.2 278,520 + +0.1 278,520 + +0.0 278,520 + +0.7 278,520 + -0.8 278,520 + -0.3 278,520 + +1.0 278,520 + +2.5 278,521 - +0.2 278,521 - +0.4 278,521 - -0.3 278,521 - +0.2 278,691 + GFF248 0.14 -1.1 278,691 + GFF248 0.14 +0.7 278,691 + GFF248 0.14 +0.6 278,692 - GFF248 0.14 +0.7 278,692 - GFF248 0.14 -0.2 278,799 + GFF248 0.19 +1.0 278,800 - GFF248 0.19 -0.5 278,800 - GFF248 0.19 -0.7 278,800 - GFF248 0.19 +0.1 278,800 - GFF248 0.19 -1.8 278,800 - GFF248 0.19 -0.1 278,850 + GFF248 0.21 -0.2 279,081 + GFF248 0.32 +0.4 279,081 + GFF248 0.32 -0.3 279,082 + GFF248 0.32 +0.0 279,082 - GFF248 0.32 +0.2
Or see this region's nucleotide sequence