Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2328 and GFF2329 are separated by 31 nucleotides GFF2329 and GFF2330 are separated by 17 nucleotides GFF2330 and GFF2331 are separated by 39 nucleotides GFF2331 and GFF2332 are separated by 111 nucleotides
GFF2328 - no description, at 379,545 to 380,186
GFF2328
GFF2329 - Phosphoglycerate mutase (EC 5.4.2.11), at 380,218 to 380,916
GFF2329
GFF2330 - FOG: TPR repeat, at 380,934 to 382,289
GFF2330
GFF2331 - Tautomerase / Tautomerase, at 382,329 to 382,727
GFF2331
GFF2332 - Transcriptional regulator, LysR family, at 382,839 to 383,699
GFF2332
Position (kb)
380
381
382
383 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 379.965 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.974 kb on + strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.975 kb on - strand, within GFF2328 at 379.983 kb on + strand, within GFF2328 at 379.983 kb on + strand, within GFF2328 at 379.984 kb on - strand, within GFF2328 at 379.984 kb on - strand, within GFF2328 at 380.034 kb on + strand, within GFF2328 at 380.034 kb on + strand, within GFF2328 at 380.034 kb on + strand, within GFF2328 at 380.035 kb on - strand, within GFF2328 at 380.035 kb on - strand, within GFF2328 at 380.035 kb on - strand, within GFF2328 at 380.036 kb on + strand, within GFF2328 at 380.036 kb on + strand, within GFF2328 at 380.037 kb on - strand, within GFF2328 at 380.662 kb on + strand, within GFF2329 at 380.662 kb on + strand, within GFF2329 at 380.663 kb on - strand, within GFF2329 at 380.881 kb on + strand at 380.881 kb on + strand at 380.881 kb on + strand at 380.881 kb on + strand at 380.881 kb on + strand at 380.882 kb on - strand at 380.882 kb on - strand at 380.882 kb on - strand at 380.882 kb on - strand at 380.972 kb on - strand at 381.095 kb on - strand, within GFF2330 at 381.095 kb on - strand, within GFF2330 at 381.341 kb on - strand, within GFF2330 at 381.341 kb on - strand, within GFF2330 at 381.346 kb on + strand, within GFF2330 at 381.346 kb on + strand, within GFF2330 at 381.346 kb on + strand, within GFF2330 at 381.347 kb on - strand, within GFF2330 at 381.347 kb on - strand, within GFF2330 at 381.347 kb on - strand, within GFF2330 at 381.398 kb on - strand, within GFF2330 at 381.490 kb on + strand, within GFF2330 at 381.490 kb on + strand, within GFF2330 at 381.491 kb on - strand, within GFF2330 at 381.491 kb on - strand, within GFF2330 at 381.523 kb on + strand, within GFF2330 at 381.523 kb on + strand, within GFF2330 at 381.781 kb on + strand, within GFF2330 at 381.808 kb on + strand, within GFF2330 at 381.808 kb on + strand, within GFF2330 at 381.809 kb on - strand, within GFF2330 at 381.809 kb on - strand, within GFF2330 at 381.809 kb on - strand, within GFF2330 at 382.004 kb on - strand, within GFF2330 at 382.117 kb on + strand, within GFF2330 at 382.118 kb on - strand, within GFF2330 at 382.381 kb on + strand, within GFF2331 at 382.382 kb on - strand, within GFF2331 at 382.382 kb on - strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.475 kb on - strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.595 kb on - strand, within GFF2331 at 382.741 kb on - strand at 382.802 kb on + strand at 383.140 kb on - strand, within GFF2332
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 379,965 + GFF2328 0.65 -0.7 379,974 + GFF2328 0.67 -2.8 379,974 + GFF2328 0.67 -0.9 379,974 + GFF2328 0.67 -0.7 379,974 + GFF2328 0.67 +0.2 379,974 + GFF2328 0.67 -0.5 379,974 + GFF2328 0.67 -0.8 379,975 - GFF2328 0.67 -0.9 379,975 - GFF2328 0.67 +1.7 379,975 - GFF2328 0.67 -0.6 379,975 - GFF2328 0.67 +2.4 379,975 - GFF2328 0.67 +0.2 379,975 - GFF2328 0.67 -0.5 379,975 - GFF2328 0.67 +0.7 379,983 + GFF2328 0.68 -0.5 379,983 + GFF2328 0.68 -0.4 379,984 - GFF2328 0.68 -0.8 379,984 - GFF2328 0.68 +0.4 380,034 + GFF2328 0.76 -1.9 380,034 + GFF2328 0.76 -0.9 380,034 + GFF2328 0.76 +0.7 380,035 - GFF2328 0.76 +0.4 380,035 - GFF2328 0.76 -0.4 380,035 - GFF2328 0.76 +0.3 380,036 + GFF2328 0.76 -1.3 380,036 + GFF2328 0.76 +0.5 380,037 - GFF2328 0.77 -0.6 380,662 + GFF2329 0.64 -0.0 380,662 + GFF2329 0.64 -0.5 380,663 - GFF2329 0.64 +1.1 380,881 + -0.2 380,881 + -0.4 380,881 + -0.1 380,881 + -0.6 380,881 + -0.2 380,882 - +2.8 380,882 - +0.0 380,882 - +0.4 380,882 - +0.9 380,972 - -0.4 381,095 - GFF2330 0.12 -2.6 381,095 - GFF2330 0.12 -0.6 381,341 - GFF2330 0.30 -0.5 381,341 - GFF2330 0.30 -0.1 381,346 + GFF2330 0.30 -0.6 381,346 + GFF2330 0.30 +0.5 381,346 + GFF2330 0.30 +0.7 381,347 - GFF2330 0.30 -0.9 381,347 - GFF2330 0.30 -0.6 381,347 - GFF2330 0.30 +0.4 381,398 - GFF2330 0.34 -0.9 381,490 + GFF2330 0.41 -0.6 381,490 + GFF2330 0.41 -0.5 381,491 - GFF2330 0.41 +0.1 381,491 - GFF2330 0.41 -0.9 381,523 + GFF2330 0.43 -0.9 381,523 + GFF2330 0.43 +1.3 381,781 + GFF2330 0.62 -1.2 381,808 + GFF2330 0.64 -0.6 381,808 + GFF2330 0.64 +0.6 381,809 - GFF2330 0.65 -3.2 381,809 - GFF2330 0.65 +0.5 381,809 - GFF2330 0.65 +0.2 382,004 - GFF2330 0.79 -2.3 382,117 + GFF2330 0.87 +0.9 382,118 - GFF2330 0.87 -0.5 382,381 + GFF2331 0.13 -0.3 382,382 - GFF2331 0.13 -1.4 382,382 - GFF2331 0.13 -0.5 382,414 + GFF2331 0.21 +0.2 382,414 + GFF2331 0.21 +2.7 382,414 + GFF2331 0.21 -0.5 382,415 - GFF2331 0.22 +0.7 382,415 - GFF2331 0.22 +1.1 382,415 - GFF2331 0.22 +0.0 382,415 - GFF2331 0.22 -0.3 382,475 - GFF2331 0.37 +1.1 382,594 + GFF2331 0.66 +0.5 382,594 + GFF2331 0.66 -0.5 382,594 + GFF2331 0.66 -1.0 382,595 - GFF2331 0.67 +0.0 382,741 - -0.1 382,802 + +0.3 383,140 - GFF2332 0.35 -0.0
Or see this region's nucleotide sequence