Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2230 and GFF2231 overlap by 4 nucleotides GFF2231 and GFF2232 are separated by 34 nucleotides GFF2232 and GFF2233 are separated by 121 nucleotides
GFF2230 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6), at 292,084 to 292,785
GFF2230
GFF2231 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6), at 292,782 to 293,429
GFF2231
GFF2232 - Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ 3-oxoadipyl-CoA thiolase (EC 2.3.1.174), at 293,464 to 294,669
GFF2232
GFF2233 - hypothetical protein, at 294,791 to 295,810
GFF2233
Position (kb) 293
294
295 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 292.592 kb on - strand, within GFF2230 at 292.653 kb on + strand, within GFF2230 at 292.794 kb on + strand at 292.794 kb on + strand at 292.794 kb on + strand at 292.795 kb on - strand at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 293.445 kb on + strand at 293.445 kb on + strand at 293.445 kb on + strand at 293.446 kb on - strand at 293.515 kb on + strand at 293.888 kb on - strand, within GFF2232 at 293.939 kb on - strand, within GFF2232 at 293.986 kb on + strand, within GFF2232 at 294.604 kb on + strand at 294.605 kb on - strand at 294.737 kb on - strand at 295.160 kb on + strand, within GFF2233 at 295.209 kb on - strand, within GFF2233 at 295.241 kb on + strand, within GFF2233 at 295.241 kb on + strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.242 kb on - strand, within GFF2233 at 295.298 kb on + strand, within GFF2233 at 295.385 kb on + strand, within GFF2233 at 295.406 kb on + strand, within GFF2233 at 295.406 kb on + strand, within GFF2233
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 292,592 - GFF2230 0.72 +0.9 292,653 + GFF2230 0.81 +0.7 292,794 + +0.4 292,794 + +2.3 292,794 + +0.1 292,795 - -0.1 292,987 - GFF2231 0.32 -0.9 292,987 - GFF2231 0.32 +0.1 292,987 - GFF2231 0.32 +0.6 292,987 - GFF2231 0.32 -2.0 293,445 + +0.2 293,445 + -1.5 293,445 + +0.2 293,446 - +0.2 293,515 + +0.4 293,888 - GFF2232 0.35 +0.3 293,939 - GFF2232 0.39 -0.5 293,986 + GFF2232 0.43 +0.5 294,604 + -0.2 294,605 - +0.2 294,737 - +0.3 295,160 + GFF2233 0.36 -1.1 295,209 - GFF2233 0.41 -0.6 295,241 + GFF2233 0.44 -0.8 295,241 + GFF2233 0.44 +4.3 295,242 - GFF2233 0.44 -0.2 295,242 - GFF2233 0.44 +0.1 295,242 - GFF2233 0.44 -0.7 295,298 + GFF2233 0.50 -1.3 295,385 + GFF2233 0.58 -0.2 295,406 + GFF2233 0.60 +1.3 295,406 + GFF2233 0.60 +0.5
Or see this region's nucleotide sequence