Strain Fitness in Variovorax sp. SCN45 around GFF216

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF214 and GFF215 are separated by 15 nucleotidesGFF215 and GFF216 are separated by 13 nucleotidesGFF216 and GFF217 are separated by 86 nucleotidesGFF217 and GFF218 are separated by 120 nucleotides GFF214 - Putative transmembrane protein, at 239,114 to 239,494 GFF214 GFF215 - RNA polymerase sigma-54 factor RpoN, at 239,510 to 240,025 GFF215 GFF216 - Transmembrane regulator protein PrtR, at 240,039 to 240,863 GFF216 GFF217 - Orf21; putative lipoprotein, at 240,950 to 241,333 GFF217 GFF218 - hypothetical protein, at 241,454 to 242,956 GFF218 Position (kb) 240 241Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 239.039 kb on - strandat 239.039 kb on - strandat 239.039 kb on - strandat 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.415 kb on - strand, within GFF214at 239.415 kb on - strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.556 kb on - strandat 239.888 kb on + strand, within GFF215at 239.888 kb on + strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 240.459 kb on + strand, within GFF216at 240.459 kb on + strand, within GFF216at 240.459 kb on + strand, within GFF216at 240.460 kb on - strand, within GFF216at 240.460 kb on - strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.766 kb on - strand, within GFF216at 240.766 kb on - strand, within GFF216at 240.846 kb on + strandat 240.861 kb on + strandat 240.861 kb on + strandat 240.872 kb on + strandat 240.873 kb on - strandat 240.873 kb on - strandat 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.079 kb on + strand, within GFF217at 241.080 kb on - strand, within GFF217at 241.080 kb on - strand, within GFF217at 241.080 kb on - strand, within GFF217at 241.191 kb on - strand, within GFF217at 241.191 kb on - strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.232 kb on + strand, within GFF217at 241.233 kb on - strand, within GFF217at 241.233 kb on - strand, within GFF217at 241.233 kb on - strand, within GFF217at 241.233 kb on - strand, within GFF217at 241.233 kb on - strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.250 kb on + strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.251 kb on - strand, within GFF217at 241.253 kb on + strand, within GFF217at 241.253 kb on + strand, within GFF217at 241.253 kb on + strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.254 kb on - strand, within GFF217at 241.772 kb on + strand, within GFF218at 241.772 kb on + strand, within GFF218at 241.773 kb on - strand, within GFF218

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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239,039 - +0.4
239,039 - +0.0
239,039 - +0.1
239,243 + GFF214 0.34 +0.9
239,243 + GFF214 0.34 +0.3
239,243 + GFF214 0.34 -0.3
239,243 + GFF214 0.34 -1.7
239,243 + GFF214 0.34 -0.2
239,244 - GFF214 0.34 -0.3
239,244 - GFF214 0.34 -1.1
239,244 - GFF214 0.34 -0.8
239,244 - GFF214 0.34 -0.4
239,244 - GFF214 0.34 +0.9
239,414 + GFF214 0.79 -0.8
239,414 + GFF214 0.79 +1.2
239,414 + GFF214 0.79 -0.2
239,414 + GFF214 0.79 +0.2
239,415 - GFF214 0.79 -0.4
239,415 - GFF214 0.79 -0.7
239,417 + GFF214 0.80 +0.6
239,417 + GFF214 0.80 -0.4
239,417 + GFF214 0.80 -1.6
239,418 - GFF214 0.80 -0.1
239,418 - GFF214 0.80 -0.5
239,418 - GFF214 0.80 +0.0
239,418 - GFF214 0.80 -0.1
239,418 - GFF214 0.80 -0.1
239,418 - GFF214 0.80 +0.4
239,556 - -2.8
239,888 + GFF215 0.73 -1.7
239,888 + GFF215 0.73 +0.1
239,889 - GFF215 0.73 -0.5
239,889 - GFF215 0.73 -0.3
239,889 - GFF215 0.73 +0.4
239,889 - GFF215 0.73 -0.6
240,459 + GFF216 0.51 -0.8
240,459 + GFF216 0.51 -1.1
240,459 + GFF216 0.51 +0.4
240,460 - GFF216 0.51 +0.5
240,460 - GFF216 0.51 -1.2
240,579 + GFF216 0.65 +0.1
240,579 + GFF216 0.65 -0.6
240,579 + GFF216 0.65 -2.9
240,579 + GFF216 0.65 +1.0
240,580 - GFF216 0.66 -0.3
240,580 - GFF216 0.66 +1.2
240,580 - GFF216 0.66 +0.1
240,580 - GFF216 0.66 -0.6
240,580 - GFF216 0.66 -1.0
240,580 - GFF216 0.66 -1.4
240,580 - GFF216 0.66 -1.0
240,672 + GFF216 0.77 -0.8
240,672 + GFF216 0.77 -0.8
240,672 + GFF216 0.77 +0.9
240,672 + GFF216 0.77 -0.2
240,672 + GFF216 0.77 -1.9
240,672 + GFF216 0.77 -0.3
240,672 + GFF216 0.77 -1.7
240,673 - GFF216 0.77 +0.5
240,673 - GFF216 0.77 -0.9
240,673 - GFF216 0.77 -0.7
240,673 - GFF216 0.77 +0.6
240,765 + GFF216 0.88 -0.7
240,765 + GFF216 0.88 -0.1
240,765 + GFF216 0.88 -0.7
240,765 + GFF216 0.88 +0.0
240,765 + GFF216 0.88 -0.3
240,766 - GFF216 0.88 -0.8
240,766 - GFF216 0.88 -0.9
240,846 + -0.6
240,861 + -0.8
240,861 + -0.6
240,872 + +1.1
240,873 - -1.0
240,873 - +0.0
241,079 + GFF217 0.34 +0.0
241,079 + GFF217 0.34 -0.4
241,079 + GFF217 0.34 +0.3
241,079 + GFF217 0.34 +0.2
241,079 + GFF217 0.34 -0.2
241,079 + GFF217 0.34 -0.7
241,079 + GFF217 0.34 -1.6
241,079 + GFF217 0.34 -0.1
241,080 - GFF217 0.34 -0.8
241,080 - GFF217 0.34 -0.7
241,080 - GFF217 0.34 -0.6
241,191 - GFF217 0.63 -0.0
241,191 - GFF217 0.63 -0.3
241,232 + GFF217 0.73 -0.4
241,232 + GFF217 0.73 -2.3
241,232 + GFF217 0.73 -1.4
241,232 + GFF217 0.73 +1.1
241,232 + GFF217 0.73 +0.2
241,232 + GFF217 0.73 +1.1
241,232 + GFF217 0.73 -0.6
241,232 + GFF217 0.73 -0.7
241,232 + GFF217 0.73 +0.6
241,232 + GFF217 0.73 +1.0
241,233 - GFF217 0.74 +1.8
241,233 - GFF217 0.74 +0.4
241,233 - GFF217 0.74 +0.3
241,233 - GFF217 0.74 -1.3
241,233 - GFF217 0.74 -0.6
241,250 + GFF217 0.78 -0.6
241,250 + GFF217 0.78 -0.4
241,250 + GFF217 0.78 +0.2
241,250 + GFF217 0.78 +0.6
241,250 + GFF217 0.78 -0.2
241,250 + GFF217 0.78 -1.1
241,251 - GFF217 0.78 -0.5
241,251 - GFF217 0.78 -1.1
241,251 - GFF217 0.78 -0.4
241,251 - GFF217 0.78 -0.7
241,251 - GFF217 0.78 -0.0
241,251 - GFF217 0.78 -1.9
241,251 - GFF217 0.78 -0.6
241,253 + GFF217 0.79 -1.1
241,253 + GFF217 0.79 -0.8
241,253 + GFF217 0.79 -0.1
241,254 - GFF217 0.79 -0.8
241,254 - GFF217 0.79 -0.0
241,254 - GFF217 0.79 -1.8
241,254 - GFF217 0.79 -0.2
241,254 - GFF217 0.79 +0.2
241,254 - GFF217 0.79 -0.0
241,772 + GFF218 0.21 -0.8
241,772 + GFF218 0.21 +0.3
241,773 - GFF218 0.21 +0.2

Or see this region's nucleotide sequence