Strain Fitness in Variovorax sp. SCN45 around GFF2074

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2072 and GFF2073 are separated by 20 nucleotidesGFF2073 and GFF2074 overlap by 4 nucleotidesGFF2074 and GFF2075 are separated by 4 nucleotides GFF2072 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 118,728 to 119,684 GFF2072 GFF2073 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 119,705 to 120,277 GFF2073 GFF2074 - hypothetical protein, at 120,274 to 121,161 GFF2074 GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161 GFF2075 Position (kb) 120 121 122Strain fitness (log2 ratio) -2 -1 0 1 2at 119.338 kb on + strand, within GFF2072at 119.338 kb on + strand, within GFF2072at 119.338 kb on + strand, within GFF2072at 119.339 kb on - strand, within GFF2072at 119.339 kb on - strand, within GFF2072at 119.485 kb on + strand, within GFF2072at 119.485 kb on + strand, within GFF2072at 119.486 kb on - strand, within GFF2072at 119.486 kb on - strand, within GFF2072at 119.486 kb on - strand, within GFF2072at 119.486 kb on - strand, within GFF2072at 119.709 kb on + strandat 119.780 kb on + strand, within GFF2073at 119.780 kb on + strand, within GFF2073at 119.781 kb on - strand, within GFF2073at 119.810 kb on + strand, within GFF2073at 119.810 kb on + strand, within GFF2073at 119.810 kb on + strand, within GFF2073at 119.810 kb on + strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 119.811 kb on - strand, within GFF2073at 120.128 kb on - strand, within GFF2073at 120.388 kb on + strand, within GFF2074at 120.409 kb on + strand, within GFF2074at 120.410 kb on - strand, within GFF2074at 120.410 kb on - strand, within GFF2074at 121.054 kb on + strand, within GFF2074at 121.055 kb on - strand, within GFF2074at 121.395 kb on + strand, within GFF2075at 121.395 kb on + strand, within GFF2075at 121.395 kb on + strand, within GFF2075at 121.395 kb on + strand, within GFF2075at 121.395 kb on + strand, within GFF2075at 121.396 kb on - strand, within GFF2075at 121.396 kb on - strand, within GFF2075at 121.396 kb on - strand, within GFF2075at 121.396 kb on - strand, within GFF2075at 121.587 kb on + strand, within GFF2075at 121.587 kb on + strand, within GFF2075at 121.588 kb on - strand, within GFF2075at 121.674 kb on + strand, within GFF2075at 121.674 kb on + strand, within GFF2075at 121.674 kb on + strand, within GFF2075at 121.674 kb on + strand, within GFF2075at 121.674 kb on + strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.675 kb on - strand, within GFF2075at 121.704 kb on + strand, within GFF2075at 121.827 kb on + strand, within GFF2075at 121.828 kb on - strand, within GFF2075at 121.828 kb on - strand, within GFF2075at 121.860 kb on + strand, within GFF2075at 121.860 kb on + strand, within GFF2075at 121.860 kb on + strand, within GFF2075at 121.860 kb on + strand, within GFF2075at 121.860 kb on + strand, within GFF2075at 122.061 kb on + strand, within GFF2075at 122.061 kb on + strand, within GFF2075at 122.062 kb on - strandat 122.062 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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119,338 + GFF2072 0.64 -1.6
119,338 + GFF2072 0.64 +0.3
119,338 + GFF2072 0.64 -0.5
119,339 - GFF2072 0.64 -0.0
119,339 - GFF2072 0.64 +0.3
119,485 + GFF2072 0.79 +0.7
119,485 + GFF2072 0.79 -0.2
119,486 - GFF2072 0.79 -0.4
119,486 - GFF2072 0.79 +0.3
119,486 - GFF2072 0.79 -0.1
119,486 - GFF2072 0.79 -0.5
119,709 + -0.7
119,780 + GFF2073 0.13 -2.1
119,780 + GFF2073 0.13 -0.1
119,781 - GFF2073 0.13 -1.0
119,810 + GFF2073 0.18 -1.0
119,810 + GFF2073 0.18 -2.0
119,810 + GFF2073 0.18 -0.4
119,810 + GFF2073 0.18 -1.9
119,811 - GFF2073 0.18 -2.2
119,811 - GFF2073 0.18 -0.5
119,811 - GFF2073 0.18 +0.1
119,811 - GFF2073 0.18 -0.7
119,811 - GFF2073 0.18 -1.9
119,811 - GFF2073 0.18 -1.1
120,128 - GFF2073 0.74 -2.0
120,388 + GFF2074 0.13 +0.1
120,409 + GFF2074 0.15 +1.4
120,410 - GFF2074 0.15 +0.2
120,410 - GFF2074 0.15 +0.4
121,054 + GFF2074 0.88 +2.5
121,055 - GFF2074 0.88 -0.6
121,395 + GFF2075 0.23 -1.2
121,395 + GFF2075 0.23 -0.0
121,395 + GFF2075 0.23 -0.9
121,395 + GFF2075 0.23 +0.6
121,395 + GFF2075 0.23 -0.4
121,396 - GFF2075 0.23 -1.1
121,396 - GFF2075 0.23 -0.9
121,396 - GFF2075 0.23 +0.3
121,396 - GFF2075 0.23 -1.1
121,587 + GFF2075 0.42 -1.4
121,587 + GFF2075 0.42 -0.1
121,588 - GFF2075 0.42 -0.9
121,674 + GFF2075 0.51 -0.3
121,674 + GFF2075 0.51 +0.3
121,674 + GFF2075 0.51 -0.2
121,674 + GFF2075 0.51 -0.5
121,674 + GFF2075 0.51 -0.8
121,675 - GFF2075 0.51 -0.4
121,675 - GFF2075 0.51 -0.7
121,675 - GFF2075 0.51 +0.3
121,675 - GFF2075 0.51 +0.1
121,675 - GFF2075 0.51 -1.2
121,675 - GFF2075 0.51 +0.6
121,704 + GFF2075 0.54 -0.6
121,827 + GFF2075 0.66 +0.3
121,828 - GFF2075 0.66 -0.9
121,828 - GFF2075 0.66 +0.1
121,860 + GFF2075 0.70 -0.1
121,860 + GFF2075 0.70 -0.3
121,860 + GFF2075 0.70 -0.5
121,860 + GFF2075 0.70 +0.0
121,860 + GFF2075 0.70 -0.0
122,061 + GFF2075 0.90 +0.5
122,061 + GFF2075 0.90 -1.6
122,062 - -0.1
122,062 - -0.1

Or see this region's nucleotide sequence