Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2040 and GFF2041 are separated by 1 nucleotides GFF2041 and GFF2042 are separated by 99 nucleotides
GFF2040 - Putative drug efflux protein, at 88,226 to 89,476
GFF2040
GFF2041 - Haloacid dehalogenase-like hydrolase, at 89,478 to 90,311
GFF2041
GFF2042 - Efflux ABC transporter, ATP-binding protein, at 90,411 to 91,406
GFF2042
Position (kb) 89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 88.719 kb on + strand, within GFF2040 at 88.719 kb on + strand, within GFF2040 at 89.318 kb on + strand, within GFF2040 at 89.319 kb on - strand, within GFF2040 at 89.319 kb on - strand, within GFF2040 at 89.319 kb on - strand, within GFF2040 at 89.319 kb on - strand, within GFF2040 at 89.319 kb on - strand, within GFF2040 at 89.396 kb on + strand at 89.397 kb on - strand at 89.397 kb on - strand at 89.397 kb on - strand at 89.397 kb on - strand at 89.426 kb on + strand at 89.426 kb on + strand at 89.426 kb on + strand at 89.427 kb on - strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.479 kb on + strand at 89.480 kb on - strand at 89.480 kb on - strand at 89.480 kb on - strand at 89.480 kb on - strand at 89.480 kb on - strand at 89.551 kb on + strand at 89.552 kb on - strand at 89.552 kb on - strand at 89.552 kb on - strand at 89.552 kb on - strand at 89.552 kb on - strand at 89.659 kb on + strand, within GFF2041 at 89.659 kb on + strand, within GFF2041 at 89.659 kb on + strand, within GFF2041 at 89.659 kb on + strand, within GFF2041 at 89.659 kb on + strand, within GFF2041 at 89.660 kb on - strand, within GFF2041 at 89.660 kb on - strand, within GFF2041 at 89.660 kb on - strand, within GFF2041 at 89.660 kb on - strand, within GFF2041 at 89.749 kb on + strand, within GFF2041 at 89.992 kb on + strand, within GFF2041 at 89.992 kb on + strand, within GFF2041 at 89.993 kb on - strand, within GFF2041 at 89.993 kb on - strand, within GFF2041 at 90.365 kb on + strand at 90.365 kb on + strand at 90.365 kb on + strand at 90.365 kb on + strand at 90.365 kb on + strand at 90.365 kb on + strand at 90.365 kb on + strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.366 kb on - strand at 90.447 kb on + strand at 90.447 kb on + strand at 90.448 kb on - strand at 90.448 kb on - strand at 90.448 kb on - strand at 90.577 kb on + strand, within GFF2042 at 90.577 kb on + strand, within GFF2042 at 90.577 kb on + strand, within GFF2042 at 90.577 kb on + strand, within GFF2042 at 90.577 kb on + strand, within GFF2042 at 90.577 kb on + strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.578 kb on - strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.786 kb on + strand, within GFF2042 at 90.787 kb on - strand, within GFF2042 at 90.787 kb on - strand, within GFF2042 at 90.787 kb on - strand, within GFF2042 at 90.787 kb on - strand, within GFF2042 at 90.787 kb on - strand, within GFF2042 at 90.787 kb on - strand, within GFF2042
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 88,719 + GFF2040 0.39 +0.0 88,719 + GFF2040 0.39 +0.9 89,318 + GFF2040 0.87 +0.1 89,319 - GFF2040 0.87 -0.2 89,319 - GFF2040 0.87 -0.5 89,319 - GFF2040 0.87 +0.7 89,319 - GFF2040 0.87 -1.2 89,319 - GFF2040 0.87 -0.4 89,396 + +0.0 89,397 - +1.7 89,397 - +0.6 89,397 - +0.9 89,397 - +0.6 89,426 + -0.1 89,426 + +0.0 89,426 + -0.3 89,427 - +0.9 89,479 + +0.0 89,479 + -0.6 89,479 + -0.7 89,479 + -0.1 89,479 + -0.1 89,479 + +0.2 89,479 + +0.1 89,479 + -0.4 89,480 - -0.5 89,480 - -0.4 89,480 - +3.3 89,480 - -0.9 89,480 - +0.1 89,551 + -1.2 89,552 - +0.0 89,552 - +0.3 89,552 - -0.4 89,552 - -0.1 89,552 - -0.1 89,659 + GFF2041 0.22 -1.0 89,659 + GFF2041 0.22 -0.4 89,659 + GFF2041 0.22 -0.0 89,659 + GFF2041 0.22 -1.9 89,659 + GFF2041 0.22 -0.5 89,660 - GFF2041 0.22 -0.3 89,660 - GFF2041 0.22 +0.1 89,660 - GFF2041 0.22 -0.8 89,660 - GFF2041 0.22 -0.3 89,749 + GFF2041 0.32 +1.7 89,992 + GFF2041 0.62 +0.7 89,992 + GFF2041 0.62 -0.3 89,993 - GFF2041 0.62 -0.7 89,993 - GFF2041 0.62 +0.5 90,365 + +0.2 90,365 + +0.1 90,365 + -0.5 90,365 + -2.4 90,365 + +2.4 90,365 + -0.3 90,365 + +0.5 90,366 - +0.1 90,366 - -0.3 90,366 - -0.5 90,366 - +0.4 90,366 - -0.9 90,366 - -0.5 90,366 - -0.7 90,447 + -0.4 90,447 + -1.1 90,448 - +0.3 90,448 - +0.6 90,448 - +0.7 90,577 + GFF2042 0.17 -0.1 90,577 + GFF2042 0.17 -1.6 90,577 + GFF2042 0.17 +0.6 90,577 + GFF2042 0.17 +0.1 90,577 + GFF2042 0.17 -3.5 90,577 + GFF2042 0.17 +0.9 90,578 - GFF2042 0.17 -1.6 90,578 - GFF2042 0.17 -1.2 90,578 - GFF2042 0.17 +0.1 90,578 - GFF2042 0.17 -0.2 90,578 - GFF2042 0.17 -1.0 90,578 - GFF2042 0.17 +0.3 90,578 - GFF2042 0.17 -0.4 90,578 - GFF2042 0.17 -0.4 90,578 - GFF2042 0.17 -0.9 90,786 + GFF2042 0.38 +0.9 90,786 + GFF2042 0.38 -0.4 90,786 + GFF2042 0.38 +2.3 90,786 + GFF2042 0.38 -0.1 90,786 + GFF2042 0.38 +0.4 90,786 + GFF2042 0.38 +0.1 90,786 + GFF2042 0.38 -0.3 90,787 - GFF2042 0.38 +3.3 90,787 - GFF2042 0.38 +0.3 90,787 - GFF2042 0.38 -0.1 90,787 - GFF2042 0.38 -0.6 90,787 - GFF2042 0.38 -0.3 90,787 - GFF2042 0.38 +0.3
Or see this region's nucleotide sequence