Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1975 and GFF1976 are separated by 153 nucleotides GFF1976 and GFF1977 are separated by 49 nucleotides GFF1977 and GFF1978 are separated by 87 nucleotides
GFF1975 - hypothetical protein, at 17,211 to 18,329
GFF1975
GFF1976 - Transcriptional regulator, AcrR family, at 18,483 to 19,274
GFF1976
GFF1977 - L-asparaginase (EC 3.5.1.1), at 19,324 to 20,394
GFF1977
GFF1978 - putative membrane-associated phospholipid phosphatase, PAP2 superfamily, at 20,482 to 21,294
GFF1978
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1 at 18.396 kb on + strand at 18.397 kb on - strand at 18.397 kb on - strand at 18.397 kb on - strand at 18.427 kb on + strand at 18.427 kb on + strand at 18.428 kb on - strand at 18.428 kb on - strand at 18.428 kb on - strand at 18.428 kb on - strand at 18.428 kb on - strand at 18.471 kb on + strand at 18.471 kb on + strand at 18.472 kb on - strand at 18.485 kb on - strand at 18.485 kb on - strand at 18.598 kb on + strand, within GFF1976 at 18.598 kb on + strand, within GFF1976 at 18.599 kb on - strand, within GFF1976 at 18.599 kb on - strand, within GFF1976 at 18.599 kb on - strand, within GFF1976 at 18.607 kb on + strand, within GFF1976 at 18.607 kb on + strand, within GFF1976 at 18.607 kb on + strand, within GFF1976 at 18.607 kb on + strand, within GFF1976 at 18.607 kb on + strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.608 kb on - strand, within GFF1976 at 18.737 kb on - strand, within GFF1976 at 18.832 kb on + strand, within GFF1976 at 18.832 kb on + strand, within GFF1976 at 18.832 kb on + strand, within GFF1976 at 18.833 kb on - strand, within GFF1976 at 18.833 kb on - strand, within GFF1976 at 18.833 kb on - strand, within GFF1976 at 18.833 kb on - strand, within GFF1976 at 18.833 kb on - strand, within GFF1976 at 18.922 kb on + strand, within GFF1976 at 18.922 kb on + strand, within GFF1976 at 18.923 kb on - strand, within GFF1976 at 18.923 kb on - strand, within GFF1976 at 18.923 kb on - strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.946 kb on + strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.947 kb on - strand, within GFF1976 at 18.949 kb on + strand, within GFF1976 at 18.949 kb on + strand, within GFF1976 at 18.950 kb on - strand, within GFF1976 at 18.950 kb on - strand, within GFF1976 at 18.950 kb on - strand, within GFF1976 at 18.950 kb on - strand, within GFF1976 at 18.950 kb on - strand, within GFF1976 at 19.209 kb on + strand at 19.209 kb on + strand at 19.209 kb on + strand at 19.209 kb on + strand at 19.209 kb on + strand at 19.210 kb on - strand at 19.210 kb on - strand at 19.210 kb on - strand at 19.210 kb on - strand at 19.210 kb on - strand at 19.229 kb on + strand at 19.229 kb on + strand at 19.229 kb on + strand at 19.229 kb on + strand at 19.230 kb on - strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.276 kb on + strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.277 kb on - strand at 19.298 kb on - strand at 19.298 kb on - strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.327 kb on + strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.328 kb on - strand at 19.483 kb on + strand, within GFF1977 at 19.483 kb on + strand, within GFF1977 at 19.483 kb on + strand, within GFF1977 at 19.483 kb on + strand, within GFF1977 at 19.484 kb on - strand, within GFF1977 at 19.484 kb on - strand, within GFF1977 at 19.484 kb on - strand, within GFF1977 at 19.484 kb on - strand, within GFF1977 at 19.484 kb on - strand, within GFF1977 at 19.705 kb on + strand, within GFF1977 at 19.804 kb on + strand, within GFF1977 at 19.805 kb on - strand, within GFF1977 at 19.805 kb on - strand, within GFF1977 at 19.975 kb on + strand, within GFF1977 at 19.976 kb on - strand, within GFF1977 at 19.976 kb on - strand, within GFF1977 at 19.976 kb on - strand, within GFF1977 at 20.071 kb on + strand, within GFF1977 at 20.072 kb on - strand, within GFF1977 at 20.072 kb on - strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.278 kb on + strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.279 kb on - strand, within GFF1977 at 20.376 kb on + strand at 20.376 kb on + strand at 20.376 kb on + strand at 20.377 kb on - strand at 20.377 kb on - strand at 20.377 kb on - strand at 20.377 kb on - strand at 20.377 kb on - strand at 20.390 kb on - strand at 20.390 kb on - strand at 20.390 kb on - strand at 20.390 kb on - strand at 20.510 kb on + strand at 20.583 kb on - strand, within GFF1978 at 20.690 kb on + strand, within GFF1978 at 20.690 kb on + strand, within GFF1978 at 20.691 kb on - strand, within GFF1978 at 20.691 kb on - strand, within GFF1978 at 20.705 kb on + strand, within GFF1978 at 20.705 kb on + strand, within GFF1978 at 20.706 kb on - strand, within GFF1978 at 20.706 kb on - strand, within GFF1978 at 20.706 kb on - strand, within GFF1978 at 20.747 kb on + strand, within GFF1978 at 20.748 kb on - strand, within GFF1978 at 21.217 kb on + strand at 21.217 kb on + strand at 21.217 kb on + strand at 21.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 18,396 + -0.7 18,397 - -2.0 18,397 - -0.6 18,397 - -0.7 18,427 + -0.5 18,427 + +0.1 18,428 - -0.1 18,428 - -1.2 18,428 - -1.3 18,428 - +0.1 18,428 - -0.3 18,471 + -0.1 18,471 + +0.7 18,472 - +0.1 18,485 - -0.2 18,485 - +0.7 18,598 + GFF1976 0.15 +0.4 18,598 + GFF1976 0.15 -0.9 18,599 - GFF1976 0.15 -0.0 18,599 - GFF1976 0.15 +0.1 18,599 - GFF1976 0.15 -0.1 18,607 + GFF1976 0.16 +0.1 18,607 + GFF1976 0.16 -0.6 18,607 + GFF1976 0.16 -0.3 18,607 + GFF1976 0.16 +0.2 18,607 + GFF1976 0.16 -0.1 18,608 - GFF1976 0.16 -0.2 18,608 - GFF1976 0.16 -0.1 18,608 - GFF1976 0.16 -0.3 18,608 - GFF1976 0.16 -0.6 18,608 - GFF1976 0.16 +0.2 18,608 - GFF1976 0.16 +0.2 18,737 - GFF1976 0.32 +0.7 18,832 + GFF1976 0.44 +0.2 18,832 + GFF1976 0.44 +0.4 18,832 + GFF1976 0.44 -0.5 18,833 - GFF1976 0.44 -0.2 18,833 - GFF1976 0.44 +0.2 18,833 - GFF1976 0.44 +0.6 18,833 - GFF1976 0.44 -1.3 18,833 - GFF1976 0.44 +0.1 18,922 + GFF1976 0.55 -0.3 18,922 + GFF1976 0.55 +0.4 18,923 - GFF1976 0.56 -0.7 18,923 - GFF1976 0.56 +0.1 18,923 - GFF1976 0.56 -0.5 18,946 + GFF1976 0.58 -0.3 18,946 + GFF1976 0.58 -0.4 18,946 + GFF1976 0.58 +0.2 18,946 + GFF1976 0.58 +0.6 18,946 + GFF1976 0.58 +1.6 18,946 + GFF1976 0.58 +0.3 18,946 + GFF1976 0.58 -0.5 18,946 + GFF1976 0.58 +0.0 18,946 + GFF1976 0.58 -0.4 18,947 - GFF1976 0.59 -0.2 18,947 - GFF1976 0.59 -0.9 18,947 - GFF1976 0.59 -1.7 18,947 - GFF1976 0.59 -0.0 18,947 - GFF1976 0.59 -0.3 18,947 - GFF1976 0.59 -1.3 18,949 + GFF1976 0.59 +0.1 18,949 + GFF1976 0.59 -0.3 18,950 - GFF1976 0.59 -0.1 18,950 - GFF1976 0.59 +0.5 18,950 - GFF1976 0.59 -0.6 18,950 - GFF1976 0.59 -0.5 18,950 - GFF1976 0.59 -0.8 19,209 + -0.5 19,209 + +0.7 19,209 + -0.3 19,209 + +0.3 19,209 + +0.5 19,210 - +0.4 19,210 - +0.4 19,210 - -0.2 19,210 - +1.4 19,210 - -0.9 19,229 + -0.1 19,229 + -0.4 19,229 + +0.4 19,229 + +0.1 19,230 - +1.7 19,276 + -0.6 19,276 + -0.3 19,276 + -0.6 19,276 + -0.5 19,276 + +1.0 19,276 + -2.6 19,276 + +0.0 19,276 + +0.7 19,277 - -0.4 19,277 - -0.4 19,277 - -0.9 19,277 - +0.4 19,277 - -0.1 19,277 - +0.2 19,277 - -0.4 19,298 - +0.7 19,298 - -1.1 19,327 + +0.2 19,327 + -0.4 19,327 + -0.2 19,327 + -0.2 19,327 + -0.6 19,327 + -0.6 19,327 + +0.5 19,327 + -0.9 19,327 + +1.7 19,327 + +0.7 19,327 + -0.6 19,327 + +0.0 19,327 + -1.1 19,327 + +0.0 19,328 - +0.1 19,328 - +0.4 19,328 - -1.0 19,328 - -0.5 19,328 - -0.5 19,328 - -0.7 19,328 - -0.7 19,328 - -0.2 19,328 - -0.9 19,328 - +0.2 19,328 - +0.3 19,328 - -0.3 19,328 - -0.1 19,483 + GFF1977 0.15 -0.5 19,483 + GFF1977 0.15 +0.3 19,483 + GFF1977 0.15 -1.2 19,483 + GFF1977 0.15 +0.1 19,484 - GFF1977 0.15 +0.4 19,484 - GFF1977 0.15 -1.3 19,484 - GFF1977 0.15 -0.4 19,484 - GFF1977 0.15 +1.7 19,484 - GFF1977 0.15 -2.0 19,705 + GFF1977 0.36 +0.9 19,804 + GFF1977 0.45 +0.7 19,805 - GFF1977 0.45 -0.9 19,805 - GFF1977 0.45 -0.2 19,975 + GFF1977 0.61 -0.7 19,976 - GFF1977 0.61 -0.7 19,976 - GFF1977 0.61 +0.4 19,976 - GFF1977 0.61 -0.3 20,071 + GFF1977 0.70 -1.6 20,072 - GFF1977 0.70 +0.0 20,072 - GFF1977 0.70 -0.8 20,278 + GFF1977 0.89 +0.5 20,278 + GFF1977 0.89 -0.8 20,278 + GFF1977 0.89 +0.1 20,278 + GFF1977 0.89 +0.1 20,278 + GFF1977 0.89 +0.6 20,278 + GFF1977 0.89 -0.8 20,278 + GFF1977 0.89 +0.2 20,278 + GFF1977 0.89 -1.1 20,278 + GFF1977 0.89 -0.6 20,278 + GFF1977 0.89 -1.4 20,279 - GFF1977 0.89 -0.9 20,279 - GFF1977 0.89 -0.0 20,279 - GFF1977 0.89 -0.4 20,279 - GFF1977 0.89 -0.3 20,279 - GFF1977 0.89 -0.3 20,279 - GFF1977 0.89 -0.9 20,279 - GFF1977 0.89 -0.1 20,279 - GFF1977 0.89 -0.4 20,279 - GFF1977 0.89 +0.3 20,279 - GFF1977 0.89 -1.3 20,279 - GFF1977 0.89 -0.4 20,279 - GFF1977 0.89 -0.5 20,376 + +0.2 20,376 + +0.1 20,376 + -0.4 20,377 - -0.1 20,377 - +0.6 20,377 - -0.2 20,377 - -0.5 20,377 - -0.9 20,390 - -0.4 20,390 - -0.8 20,390 - -0.3 20,390 - -0.8 20,510 + +0.0 20,583 - GFF1978 0.12 -0.4 20,690 + GFF1978 0.26 -0.9 20,690 + GFF1978 0.26 -0.1 20,691 - GFF1978 0.26 +0.8 20,691 - GFF1978 0.26 -1.3 20,705 + GFF1978 0.27 -0.5 20,705 + GFF1978 0.27 +1.5 20,706 - GFF1978 0.28 +0.7 20,706 - GFF1978 0.28 -0.6 20,706 - GFF1978 0.28 -0.1 20,747 + GFF1978 0.33 -0.2 20,748 - GFF1978 0.33 -0.1 21,217 + +0.1 21,217 + +0.1 21,217 + -0.4 21,378 + +0.8
Or see this region's nucleotide sequence