Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1784 and GFF1785 are separated by 112 nucleotides GFF1785 and GFF1786 overlap by 4 nucleotides GFF1786 and GFF1787 are separated by 8 nucleotides
GFF1784 - no description, at 445,163 to 445,852
GFF1784
GFF1785 - Two-component transcriptional response regulator, OmpR family, at 445,965 to 446,654
GFF1785
GFF1786 - hypothetical protein, at 446,651 to 448,003
GFF1786
GFF1787 - no description, at 448,012 to 448,167
GFF1787
Position (kb) 446
447
448
449 Strain fitness (log2 ratio) -1
0
1 at 445.716 kb on + strand, within GFF1784 at 445.717 kb on - strand, within GFF1784 at 445.803 kb on + strand at 445.804 kb on - strand at 445.867 kb on + strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.917 kb on + strand at 446.256 kb on + strand, within GFF1785 at 446.617 kb on - strand at 446.617 kb on - strand at 447.374 kb on + strand, within GFF1786 at 447.374 kb on + strand, within GFF1786 at 447.374 kb on + strand, within GFF1786 at 447.375 kb on - strand, within GFF1786 at 447.375 kb on - strand, within GFF1786 at 447.819 kb on + strand, within GFF1786 at 448.165 kb on + strand at 448.165 kb on + strand at 448.166 kb on - strand at 448.174 kb on + strand at 448.174 kb on + strand at 448.175 kb on - strand at 448.175 kb on - strand at 448.371 kb on - strand at 448.474 kb on + strand at 448.612 kb on + strand at 448.612 kb on + strand at 448.612 kb on + strand at 448.613 kb on - strand at 448.844 kb on - strand at 448.942 kb on + strand at 448.946 kb on - strand at 448.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 445,716 + GFF1784 0.80 +0.1 445,717 - GFF1784 0.80 +1.2 445,803 + +0.3 445,804 - -0.1 445,867 + +0.4 445,868 - +0.3 445,868 - -0.6 445,868 - +0.7 445,868 - +0.2 445,917 + +0.8 446,256 + GFF1785 0.42 -1.5 446,617 - +0.0 446,617 - -0.4 447,374 + GFF1786 0.53 +0.8 447,374 + GFF1786 0.53 +0.4 447,374 + GFF1786 0.53 +0.3 447,375 - GFF1786 0.54 -0.5 447,375 - GFF1786 0.54 -0.1 447,819 + GFF1786 0.86 -0.2 448,165 + +0.3 448,165 + +0.1 448,166 - +1.3 448,174 + +0.3 448,174 + +0.5 448,175 - +1.2 448,175 - +0.0 448,371 - -0.4 448,474 + -1.5 448,612 + -0.0 448,612 + -1.7 448,612 + -0.8 448,613 - -0.9 448,844 - -0.1 448,942 + +1.2 448,946 - -0.4 448,946 - -0.2
Or see this region's nucleotide sequence