Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1601 and GFF1602 are separated by 23 nucleotides GFF1602 and GFF1603 overlap by 4 nucleotides GFF1603 and GFF1604 are separated by 1 nucleotides
GFF1601 - no description, at 199,020 to 200,219
GFF1601
GFF1602 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 200,243 to 200,965
GFF1602
GFF1603 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 200,962 to 201,729
GFF1603
GFF1604 - Prolidase (EC 3.4.13.9), at 201,731 to 202,996
GFF1604
Position (kb) 200
201 Strain fitness (log2 ratio) -2
-1
0
1
2 at 199.303 kb on + strand, within GFF1601 at 199.303 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.403 kb on - strand, within GFF1601 at 199.487 kb on - strand, within GFF1601 at 199.499 kb on - strand, within GFF1601 at 199.512 kb on + strand, within GFF1601 at 199.534 kb on + strand, within GFF1601 at 199.535 kb on - strand, within GFF1601 at 199.615 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.676 kb on - strand, within GFF1601 at 199.795 kb on + strand, within GFF1601 at 199.796 kb on - strand, within GFF1601 at 199.903 kb on + strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 200.213 kb on + strand at 200.652 kb on + strand, within GFF1602 at 200.652 kb on + strand, within GFF1602 at 200.985 kb on - strand at 200.985 kb on - strand at 201.042 kb on - strand, within GFF1603 at 201.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 199,303 + GFF1601 0.24 +1.7 199,303 + GFF1601 0.24 +0.3 199,318 + GFF1601 0.25 -0.1 199,318 + GFF1601 0.25 +0.1 199,319 - GFF1601 0.25 +0.3 199,319 - GFF1601 0.25 +0.5 199,363 + GFF1601 0.29 -0.6 199,363 + GFF1601 0.29 +0.1 199,364 - GFF1601 0.29 +1.9 199,364 - GFF1601 0.29 -2.0 199,403 - GFF1601 0.32 +1.7 199,487 - GFF1601 0.39 +0.1 199,499 - GFF1601 0.40 -0.9 199,512 + GFF1601 0.41 -0.9 199,534 + GFF1601 0.43 -0.7 199,535 - GFF1601 0.43 -1.1 199,615 + GFF1601 0.50 -0.0 199,675 + GFF1601 0.55 +0.1 199,675 + GFF1601 0.55 -0.5 199,676 - GFF1601 0.55 -0.2 199,795 + GFF1601 0.65 +0.3 199,796 - GFF1601 0.65 -1.0 199,903 + GFF1601 0.74 -2.3 199,904 - GFF1601 0.74 +1.8 199,904 - GFF1601 0.74 -0.0 199,904 - GFF1601 0.74 +0.2 200,213 + +0.1 200,652 + GFF1602 0.57 -0.6 200,652 + GFF1602 0.57 -1.8 200,985 - +0.2 200,985 - -0.9 201,042 - GFF1603 0.10 -0.6 201,758 + -0.9
Or see this region's nucleotide sequence