Strain Fitness in Variovorax sp. SCN45 around GFF156

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF155 and GFF156 are separated by 16 nucleotidesGFF156 and GFF157 overlap by 4 nucleotidesGFF157 and GFF158 overlap by 4 nucleotides GFF155 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 183,212 to 184,783 GFF155 GFF156 - Glycosyl transferase, group 2 family, at 184,800 to 185,498 GFF156 GFF157 - Methyltransferase type 12, at 185,495 to 186,100 GFF157 GFF158 - LmbE-like protein, at 186,097 to 186,858 GFF158 Position (kb) 184 185 186Strain fitness (log2 ratio) -3 -2 -1 0 1at 183.812 kb on + strand, within GFF155at 183.812 kb on + strand, within GFF155at 183.812 kb on + strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.839 kb on + strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.876 kb on - strand, within GFF155at 183.876 kb on - strand, within GFF155at 184.215 kb on - strand, within GFF155at 184.215 kb on - strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.245 kb on - strand, within GFF155at 184.286 kb on + strand, within GFF155at 184.286 kb on + strand, within GFF155at 184.287 kb on - strand, within GFF155at 184.287 kb on - strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.433 kb on + strand, within GFF155at 184.434 kb on - strand, within GFF155at 184.434 kb on - strand, within GFF155at 184.448 kb on + strand, within GFF155at 184.448 kb on + strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.469 kb on + strand, within GFF155at 184.470 kb on - strand, within GFF155at 184.470 kb on - strand, within GFF155at 184.499 kb on + strand, within GFF155at 184.500 kb on - strand, within GFF155at 184.500 kb on - strand, within GFF155at 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.907 kb on + strand, within GFF156at 184.908 kb on - strand, within GFF156at 184.908 kb on - strand, within GFF156at 185.083 kb on + strand, within GFF156at 185.083 kb on + strand, within GFF156at 185.084 kb on - strand, within GFF156at 185.084 kb on - strand, within GFF156at 185.746 kb on - strand, within GFF157at 185.958 kb on + strand, within GFF157at 185.958 kb on + strand, within GFF157at 185.959 kb on - strand, within GFF157at 185.971 kb on - strand, within GFF157at 185.971 kb on - strand, within GFF157at 186.021 kb on + strand, within GFF157at 186.022 kb on - strand, within GFF157at 186.042 kb on + strandat 186.043 kb on - strandat 186.043 kb on - strandat 186.063 kb on + strandat 186.063 kb on + strandat 186.063 kb on + strandat 186.063 kb on + strandat 186.064 kb on - strandat 186.064 kb on - strandat 186.064 kb on - strandat 186.078 kb on + strandat 186.078 kb on + strandat 186.116 kb on + strandat 186.116 kb on + strandat 186.117 kb on - strandat 186.117 kb on - strandat 186.117 kb on - strandat 186.117 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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183,812 + GFF155 0.38 -0.7
183,812 + GFF155 0.38 -0.3
183,812 + GFF155 0.38 +0.4
183,813 - GFF155 0.38 -1.1
183,813 - GFF155 0.38 -0.4
183,813 - GFF155 0.38 -0.1
183,813 - GFF155 0.38 -0.4
183,813 - GFF155 0.38 -0.7
183,839 + GFF155 0.40 +0.4
183,840 - GFF155 0.40 -0.7
183,840 - GFF155 0.40 +0.2
183,840 - GFF155 0.40 -1.4
183,840 - GFF155 0.40 +0.2
183,840 - GFF155 0.40 -0.5
183,875 + GFF155 0.42 -1.1
183,875 + GFF155 0.42 -0.2
183,875 + GFF155 0.42 -0.5
183,876 - GFF155 0.42 +0.2
183,876 - GFF155 0.42 -0.3
184,215 - GFF155 0.64 -0.3
184,215 - GFF155 0.64 -0.0
184,244 + GFF155 0.66 -1.2
184,244 + GFF155 0.66 -0.8
184,244 + GFF155 0.66 -1.0
184,245 - GFF155 0.66 -1.1
184,286 + GFF155 0.68 +1.6
184,286 + GFF155 0.68 -0.4
184,287 - GFF155 0.68 -1.6
184,287 - GFF155 0.68 -0.0
184,340 + GFF155 0.72 -1.4
184,340 + GFF155 0.72 +0.5
184,340 + GFF155 0.72 -1.1
184,341 - GFF155 0.72 +0.6
184,341 - GFF155 0.72 +0.5
184,341 - GFF155 0.72 +1.1
184,341 - GFF155 0.72 -0.2
184,433 + GFF155 0.78 -0.4
184,434 - GFF155 0.78 +0.2
184,434 - GFF155 0.78 -0.3
184,448 + GFF155 0.79 +1.2
184,448 + GFF155 0.79 -0.3
184,449 - GFF155 0.79 -0.2
184,449 - GFF155 0.79 -0.4
184,449 - GFF155 0.79 +1.0
184,449 - GFF155 0.79 -0.2
184,469 + GFF155 0.80 +0.4
184,470 - GFF155 0.80 +1.4
184,470 - GFF155 0.80 +0.6
184,499 + GFF155 0.82 +0.2
184,500 - GFF155 0.82 +0.8
184,500 - GFF155 0.82 -0.6
184,657 + +0.2
184,657 + -0.1
184,657 + -0.5
184,657 + +0.5
184,657 + +0.3
184,658 - -0.9
184,658 - +0.1
184,658 - +1.2
184,658 - +0.3
184,658 - -1.0
184,907 + GFF156 0.15 +1.5
184,908 - GFF156 0.15 +0.4
184,908 - GFF156 0.15 +0.6
185,083 + GFF156 0.40 +1.2
185,083 + GFF156 0.40 -0.6
185,084 - GFF156 0.41 -0.0
185,084 - GFF156 0.41 +0.6
185,746 - GFF157 0.41 +0.8
185,958 + GFF157 0.76 -0.4
185,958 + GFF157 0.76 +0.5
185,959 - GFF157 0.77 +0.1
185,971 - GFF157 0.79 +0.9
185,971 - GFF157 0.79 +0.5
186,021 + GFF157 0.87 -2.8
186,022 - GFF157 0.87 -0.7
186,042 + -0.3
186,043 - -0.3
186,043 - +0.1
186,063 + -0.0
186,063 + +0.2
186,063 + +0.5
186,063 + -0.6
186,064 - +0.5
186,064 - +1.2
186,064 - -2.0
186,078 + -0.2
186,078 + -0.5
186,116 + -1.6
186,116 + -1.0
186,117 - -0.6
186,117 - +0.6
186,117 - -0.0
186,117 - -1.6

Or see this region's nucleotide sequence