Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1495 and GFF1496 overlap by 4 nucleotides GFF1496 and GFF1497 are separated by 38 nucleotides GFF1497 and GFF1498 overlap by 4 nucleotides GFF1498 and GFF1499 are separated by 6 nucleotides
GFF1495 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 88,196 to 89,122
GFF1495
GFF1496 - Demethylmenaquinone methyltransferase-like protein, at 89,119 to 89,808
GFF1496
GFF1497 - BUG/TctC family periplasmic protein, at 89,847 to 90,833
GFF1497
GFF1498 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 90,830 to 91,750
GFF1498
GFF1499 - BUG/TctC family periplasmic protein, at 91,757 to 92,743
GFF1499
Position (kb) 89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 89.046 kb on + strand at 89.046 kb on + strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 90.055 kb on + strand, within GFF1497 at 90.115 kb on - strand, within GFF1497 at 90.268 kb on + strand, within GFF1497 at 90.269 kb on - strand, within GFF1497 at 90.493 kb on + strand, within GFF1497 at 90.493 kb on + strand, within GFF1497 at 90.494 kb on - strand, within GFF1497 at 90.494 kb on - strand, within GFF1497 at 90.560 kb on - strand, within GFF1497 at 90.870 kb on + strand at 90.870 kb on + strand at 90.871 kb on - strand at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.214 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 89,046 + +0.2 89,046 + +0.2 89,047 - +0.1 89,047 - -0.3 89,047 - +0.3 89,047 - -0.6 89,051 + -0.8 89,051 + -0.9 89,051 + +0.8 89,051 + +0.6 89,051 + -0.2 89,051 + +0.5 89,051 + -0.2 89,051 + +0.3 89,051 + -0.1 89,051 + -0.7 89,051 + -0.2 89,052 - -2.3 89,052 - +0.8 89,052 - -0.4 89,052 - +0.4 89,052 - -0.2 89,052 - -0.5 89,052 - +0.3 89,052 - +0.4 89,052 - -0.4 89,052 - -1.4 89,052 - -2.3 90,055 + GFF1497 0.21 -0.2 90,115 - GFF1497 0.27 -0.2 90,268 + GFF1497 0.43 +0.3 90,269 - GFF1497 0.43 -1.1 90,493 + GFF1497 0.65 -0.9 90,493 + GFF1497 0.65 -1.1 90,494 - GFF1497 0.66 -1.1 90,494 - GFF1497 0.66 -3.3 90,560 - GFF1497 0.72 -0.4 90,870 + -0.1 90,870 + +1.1 90,871 - -0.1 91,104 + GFF1498 0.30 -0.7 91,104 + GFF1498 0.30 -0.0 91,104 + GFF1498 0.30 -0.2 91,104 + GFF1498 0.30 -1.4 91,105 - GFF1498 0.30 -0.2 91,105 - GFF1498 0.30 -0.5 91,105 - GFF1498 0.30 +0.8 91,214 + GFF1498 0.42 -0.4 91,509 + GFF1498 0.74 +0.1 91,509 + GFF1498 0.74 +0.2
Or see this region's nucleotide sequence