Strain Fitness in Variovorax sp. SCN45 around GFF140

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF139 and GFF140 are separated by 121 nucleotidesGFF140 and GFF141 are separated by 24 nucleotides GFF139 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), at 167,049 to 168,284 GFF139 GFF140 - Putative transport protein, at 168,406 to 169,347 GFF140 GFF141 - Transcriptional regulator, AraC family, at 169,372 to 170,415 GFF141 Position (kb) 168 169 170Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 167.425 kb on + strand, within GFF139at 167.599 kb on + strand, within GFF139at 167.599 kb on + strand, within GFF139at 167.599 kb on + strand, within GFF139at 167.869 kb on + strand, within GFF139at 167.869 kb on + strand, within GFF139at 167.869 kb on + strand, within GFF139at 167.870 kb on - strand, within GFF139at 167.870 kb on - strand, within GFF139at 167.870 kb on - strand, within GFF139at 167.870 kb on - strand, within GFF139at 167.899 kb on + strand, within GFF139at 167.899 kb on + strand, within GFF139at 167.899 kb on + strand, within GFF139at 167.899 kb on + strand, within GFF139at 167.900 kb on - strand, within GFF139at 167.920 kb on + strand, within GFF139at 167.921 kb on - strand, within GFF139at 167.921 kb on - strand, within GFF139at 167.921 kb on - strand, within GFF139at 167.968 kb on + strand, within GFF139at 167.969 kb on - strand, within GFF139at 167.969 kb on - strand, within GFF139at 168.086 kb on - strand, within GFF139at 168.362 kb on + strandat 168.407 kb on + strandat 168.407 kb on + strandat 168.587 kb on - strand, within GFF140at 168.587 kb on - strand, within GFF140at 168.587 kb on - strand, within GFF140at 168.606 kb on - strand, within GFF140at 168.606 kb on - strand, within GFF140at 168.664 kb on + strand, within GFF140at 168.665 kb on - strand, within GFF140at 168.815 kb on + strand, within GFF140at 168.815 kb on + strand, within GFF140at 168.816 kb on - strand, within GFF140at 168.878 kb on + strand, within GFF140at 168.878 kb on + strand, within GFF140at 168.879 kb on - strand, within GFF140at 169.008 kb on - strand, within GFF140at 169.008 kb on - strand, within GFF140at 169.008 kb on - strand, within GFF140at 169.125 kb on - strand, within GFF140at 169.125 kb on - strand, within GFF140at 169.125 kb on - strand, within GFF140at 169.322 kb on + strandat 169.472 kb on + strandat 169.502 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.694 kb on + strand, within GFF141at 169.695 kb on - strand, within GFF141at 169.695 kb on - strand, within GFF141at 169.695 kb on - strand, within GFF141at 169.695 kb on - strand, within GFF141at 169.695 kb on - strand, within GFF141at 169.799 kb on + strand, within GFF141at 169.799 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.913 kb on + strand, within GFF141at 169.914 kb on - strand, within GFF141at 169.914 kb on - strand, within GFF141at 169.914 kb on - strand, within GFF141at 169.956 kb on + strand, within GFF141at 169.956 kb on + strand, within GFF141at 169.956 kb on + strand, within GFF141at 169.957 kb on - strand, within GFF141at 169.957 kb on - strand, within GFF141at 170.015 kb on + strand, within GFF141at 170.016 kb on - strand, within GFF141at 170.082 kb on - strand, within GFF141at 170.082 kb on - strand, within GFF141at 170.096 kb on + strand, within GFF141at 170.096 kb on + strand, within GFF141at 170.096 kb on + strand, within GFF141at 170.097 kb on - strand, within GFF141at 170.097 kb on - strand, within GFF141at 170.097 kb on - strand, within GFF141at 170.102 kb on + strand, within GFF141at 170.102 kb on + strand, within GFF141at 170.102 kb on + strand, within GFF141at 170.102 kb on + strand, within GFF141at 170.102 kb on + strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141at 170.103 kb on - strand, within GFF141

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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167,425 + GFF139 0.30 +0.2
167,599 + GFF139 0.44 +0.0
167,599 + GFF139 0.44 +0.9
167,599 + GFF139 0.44 -0.4
167,869 + GFF139 0.66 +0.4
167,869 + GFF139 0.66 +0.4
167,869 + GFF139 0.66 -0.0
167,870 - GFF139 0.66 -0.8
167,870 - GFF139 0.66 +0.1
167,870 - GFF139 0.66 +0.1
167,870 - GFF139 0.66 +0.7
167,899 + GFF139 0.69 +0.7
167,899 + GFF139 0.69 -0.2
167,899 + GFF139 0.69 -0.0
167,899 + GFF139 0.69 +0.3
167,900 - GFF139 0.69 -0.5
167,920 + GFF139 0.70 -0.1
167,921 - GFF139 0.71 -1.8
167,921 - GFF139 0.71 +1.2
167,921 - GFF139 0.71 -0.4
167,968 + GFF139 0.74 +2.2
167,969 - GFF139 0.74 -1.2
167,969 - GFF139 0.74 +0.2
168,086 - GFF139 0.84 +1.2
168,362 + +0.9
168,407 + +0.0
168,407 + -0.1
168,587 - GFF140 0.19 +1.0
168,587 - GFF140 0.19 -0.1
168,587 - GFF140 0.19 +0.2
168,606 - GFF140 0.21 +0.3
168,606 - GFF140 0.21 +1.2
168,664 + GFF140 0.27 +2.0
168,665 - GFF140 0.27 -0.0
168,815 + GFF140 0.43 -0.0
168,815 + GFF140 0.43 -1.8
168,816 - GFF140 0.44 +0.9
168,878 + GFF140 0.50 -0.3
168,878 + GFF140 0.50 -1.8
168,879 - GFF140 0.50 -0.4
169,008 - GFF140 0.64 -0.7
169,008 - GFF140 0.64 -0.6
169,008 - GFF140 0.64 +0.0
169,125 - GFF140 0.76 +0.6
169,125 - GFF140 0.76 -0.8
169,125 - GFF140 0.76 -0.8
169,322 + -0.1
169,472 + +0.5
169,502 + GFF141 0.12 -0.4
169,694 + GFF141 0.31 -0.7
169,694 + GFF141 0.31 -1.1
169,694 + GFF141 0.31 -0.9
169,694 + GFF141 0.31 -0.6
169,694 + GFF141 0.31 -2.0
169,694 + GFF141 0.31 -0.2
169,695 - GFF141 0.31 +0.5
169,695 - GFF141 0.31 -0.6
169,695 - GFF141 0.31 +0.6
169,695 - GFF141 0.31 -0.2
169,695 - GFF141 0.31 -0.5
169,799 + GFF141 0.41 +0.2
169,799 + GFF141 0.41 +0.5
169,913 + GFF141 0.52 -2.1
169,913 + GFF141 0.52 +0.8
169,913 + GFF141 0.52 -1.6
169,913 + GFF141 0.52 +0.7
169,913 + GFF141 0.52 +0.1
169,913 + GFF141 0.52 +0.5
169,913 + GFF141 0.52 -0.3
169,913 + GFF141 0.52 -1.1
169,913 + GFF141 0.52 -0.4
169,914 - GFF141 0.52 +1.5
169,914 - GFF141 0.52 +0.3
169,914 - GFF141 0.52 -1.0
169,956 + GFF141 0.56 +0.1
169,956 + GFF141 0.56 -0.0
169,956 + GFF141 0.56 -0.4
169,957 - GFF141 0.56 +0.2
169,957 - GFF141 0.56 -1.1
170,015 + GFF141 0.62 -0.7
170,016 - GFF141 0.62 -0.4
170,082 - GFF141 0.68 -1.0
170,082 - GFF141 0.68 -0.5
170,096 + GFF141 0.69 +0.1
170,096 + GFF141 0.69 +0.6
170,096 + GFF141 0.69 +0.1
170,097 - GFF141 0.69 -0.4
170,097 - GFF141 0.69 -0.7
170,097 - GFF141 0.69 +1.2
170,102 + GFF141 0.70 +0.2
170,102 + GFF141 0.70 -0.9
170,102 + GFF141 0.70 +1.2
170,102 + GFF141 0.70 -0.6
170,102 + GFF141 0.70 -0.0
170,103 - GFF141 0.70 -3.3
170,103 - GFF141 0.70 -0.1
170,103 - GFF141 0.70 -0.2
170,103 - GFF141 0.70 +1.0
170,103 - GFF141 0.70 -0.1
170,103 - GFF141 0.70 -0.2
170,103 - GFF141 0.70 -0.4

Or see this region's nucleotide sequence