Strain Fitness in Variovorax sp. SCN45 around GFF116

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF114 and GFF115 are separated by 1 nucleotidesGFF115 and GFF116 are separated by 3 nucleotidesGFF116 and GFF117 are separated by 188 nucleotidesGFF117 and GFF118 are separated by 13 nucleotides GFF114 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 142,732 to 143,388 GFF114 GFF115 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 143,390 to 144,055 GFF115 GFF116 - RidA/YER057c/UK114 superfamily, group 1, at 144,059 to 144,571 GFF116 GFF117 - no description, at 144,760 to 145,539 GFF117 GFF118 - putative membrane protein, at 145,553 to 146,578 GFF118 Position (kb) 144 145Strain fitness (log2 ratio) -3 -2 -1 0 1at 143.174 kb on + strand, within GFF114at 143.174 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.225 kb on + strand, within GFF114at 143.226 kb on - strand, within GFF114at 143.226 kb on - strand, within GFF114at 143.226 kb on - strand, within GFF114at 143.463 kb on + strand, within GFF115at 143.463 kb on + strand, within GFF115at 143.464 kb on - strand, within GFF115at 143.464 kb on - strand, within GFF115at 143.464 kb on - strand, within GFF115at 143.484 kb on + strand, within GFF115at 143.484 kb on + strand, within GFF115at 143.485 kb on - strand, within GFF115at 143.485 kb on - strand, within GFF115at 143.732 kb on + strand, within GFF115at 143.745 kb on + strand, within GFF115at 143.745 kb on + strand, within GFF115at 143.745 kb on + strand, within GFF115at 143.746 kb on - strand, within GFF115at 143.746 kb on - strand, within GFF115at 143.746 kb on - strand, within GFF115at 143.827 kb on - strand, within GFF115at 143.827 kb on - strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.977 kb on - strand, within GFF115at 143.977 kb on - strand, within GFF115at 144.019 kb on - strandat 144.048 kb on + strandat 144.048 kb on + strandat 144.333 kb on + strand, within GFF116at 144.333 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.712 kb on - strandat 144.872 kb on - strand, within GFF117at 145.120 kb on + strand, within GFF117at 145.120 kb on + strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.273 kb on + strand, within GFF117at 145.273 kb on + strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.367 kb on - strand, within GFF117at 145.367 kb on - strand, within GFF117at 145.498 kb on + strandat 145.499 kb on - strandat 145.499 kb on - strandat 145.499 kb on - strandat 145.531 kb on + strandat 145.532 kb on - strandat 145.532 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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143,174 + GFF114 0.67 +0.2
143,174 + GFF114 0.67 -0.3
143,225 + GFF114 0.75 -0.2
143,225 + GFF114 0.75 -0.4
143,225 + GFF114 0.75 -1.4
143,225 + GFF114 0.75 +0.2
143,225 + GFF114 0.75 -1.1
143,225 + GFF114 0.75 +0.6
143,225 + GFF114 0.75 -0.3
143,226 - GFF114 0.75 -0.4
143,226 - GFF114 0.75 -1.1
143,226 - GFF114 0.75 +1.0
143,463 + GFF115 0.11 -0.2
143,463 + GFF115 0.11 +0.3
143,464 - GFF115 0.11 +0.4
143,464 - GFF115 0.11 -0.5
143,464 - GFF115 0.11 -0.8
143,484 + GFF115 0.14 -0.1
143,484 + GFF115 0.14 -0.1
143,485 - GFF115 0.14 +0.6
143,485 - GFF115 0.14 +0.4
143,732 + GFF115 0.51 -0.4
143,745 + GFF115 0.53 -1.4
143,745 + GFF115 0.53 +0.3
143,745 + GFF115 0.53 -1.3
143,746 - GFF115 0.53 +0.3
143,746 - GFF115 0.53 -0.3
143,746 - GFF115 0.53 -0.4
143,827 - GFF115 0.66 -0.4
143,827 - GFF115 0.66 +0.2
143,877 + GFF115 0.73 +0.8
143,877 + GFF115 0.73 +0.5
143,877 + GFF115 0.73 +1.6
143,877 + GFF115 0.73 +0.1
143,877 + GFF115 0.73 -0.5
143,877 + GFF115 0.73 -1.4
143,877 + GFF115 0.73 -0.1
143,878 - GFF115 0.73 -0.0
143,878 - GFF115 0.73 -0.8
143,878 - GFF115 0.73 +0.6
143,878 - GFF115 0.73 -0.1
143,977 - GFF115 0.88 -1.1
143,977 - GFF115 0.88 -0.7
144,019 - -0.4
144,048 + +0.2
144,048 + +0.5
144,333 + GFF116 0.53 -0.3
144,333 + GFF116 0.53 -1.0
144,417 + GFF116 0.70 -0.1
144,417 + GFF116 0.70 +0.0
144,417 + GFF116 0.70 -0.5
144,417 + GFF116 0.70 -0.9
144,417 + GFF116 0.70 +0.2
144,418 - GFF116 0.70 -1.4
144,418 - GFF116 0.70 -1.4
144,418 - GFF116 0.70 -0.7
144,418 - GFF116 0.70 -0.5
144,418 - GFF116 0.70 +0.4
144,685 + -1.9
144,685 + -0.2
144,685 + -0.4
144,685 + -2.1
144,685 + +0.2
144,685 + +0.3
144,685 + +0.1
144,686 - -0.1
144,686 - -0.3
144,686 - +0.9
144,686 - +0.2
144,686 - -0.6
144,686 - -2.0
144,686 - -0.4
144,686 - -1.4
144,712 - -0.3
144,872 - GFF117 0.14 +0.5
145,120 + GFF117 0.46 +0.2
145,120 + GFF117 0.46 +0.8
145,121 - GFF117 0.46 -0.4
145,121 - GFF117 0.46 -1.3
145,121 - GFF117 0.46 -0.4
145,273 + GFF117 0.66 +0.2
145,273 + GFF117 0.66 -3.0
145,274 - GFF117 0.66 -1.2
145,274 - GFF117 0.66 -1.5
145,274 - GFF117 0.66 +0.5
145,367 - GFF117 0.78 -0.6
145,367 - GFF117 0.78 -1.4
145,498 + -0.8
145,499 - +0.3
145,499 - -1.0
145,499 - -1.3
145,531 + +0.4
145,532 - -0.7
145,532 - -0.7

Or see this region's nucleotide sequence