Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1040 and GFF1041 are separated by 18 nucleotides GFF1041 and GFF1043 overlap by 4 nucleotides GFF1043 and GFF1042 overlap by 2 nucleotides GFF1042 and GFF1044 are separated by 13 nucleotides GFF1044 and GFF1045 are separated by 31 nucleotides
GFF1040 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 357,342 to 358,244
GFF1040
GFF1041 - Transcriptional regulator, AsnC family, at 358,263 to 358,721
GFF1041
GFF1043 - no description, at 358,718 to 358,846
GFF1043
GFF1042 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 358,845 to 359,453
GFF1042
GFF1044 - hypothetical protein, at 359,467 to 359,856
GFF1044
GFF1045 - Putative phosphatase, at 359,888 to 361,765
GFF1045
Position (kb) 358
359
360 Strain fitness (log2 ratio) -2
-1
0
1
2 at 358.155 kb on + strand at 358.155 kb on + strand at 358.155 kb on + strand at 358.155 kb on + strand at 358.156 kb on - strand at 358.156 kb on - strand at 358.251 kb on - strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.480 kb on + strand, within GFF1041 at 358.480 kb on + strand, within GFF1041 at 358.481 kb on - strand, within GFF1041 at 358.567 kb on + strand, within GFF1041 at 358.567 kb on + strand, within GFF1041 at 358.813 kb on + strand, within GFF1043 at 359.166 kb on + strand, within GFF1042 at 359.166 kb on + strand, within GFF1042 at 359.166 kb on + strand, within GFF1042 at 359.167 kb on - strand, within GFF1042 at 359.244 kb on + strand, within GFF1042 at 359.245 kb on - strand, within GFF1042 at 359.400 kb on + strand at 359.400 kb on + strand at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.724 kb on - strand, within GFF1044 at 359.724 kb on - strand, within GFF1044 at 359.735 kb on + strand, within GFF1044 at 359.735 kb on + strand, within GFF1044 at 359.736 kb on - strand, within GFF1044 at 360.168 kb on + strand, within GFF1045 at 360.168 kb on + strand, within GFF1045 at 360.168 kb on + strand, within GFF1045 at 360.168 kb on + strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.169 kb on - strand, within GFF1045 at 360.282 kb on + strand, within GFF1045
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 358,155 + -2.2 358,155 + -0.4 358,155 + -0.1 358,155 + -0.5 358,156 - +0.2 358,156 - +0.2 358,251 - -0.1 358,264 + +0.4 358,264 + -1.5 358,264 + +0.1 358,264 + +0.4 358,265 - +1.0 358,265 - -2.0 358,265 - -0.5 358,265 - -1.2 358,480 + GFF1041 0.47 -0.1 358,480 + GFF1041 0.47 -1.3 358,481 - GFF1041 0.47 -0.1 358,567 + GFF1041 0.66 -1.2 358,567 + GFF1041 0.66 -0.6 358,813 + GFF1043 0.74 +0.4 359,166 + GFF1042 0.53 +0.1 359,166 + GFF1042 0.53 +0.4 359,166 + GFF1042 0.53 +0.4 359,167 - GFF1042 0.53 -1.2 359,244 + GFF1042 0.66 +0.0 359,245 - GFF1042 0.66 -1.0 359,400 + +0.8 359,400 + +0.9 359,546 + GFF1044 0.20 +0.1 359,546 + GFF1044 0.20 -0.3 359,546 + GFF1044 0.20 -0.7 359,546 + GFF1044 0.20 +0.5 359,546 + GFF1044 0.20 +0.2 359,546 + GFF1044 0.20 +0.2 359,547 - GFF1044 0.21 +0.1 359,547 - GFF1044 0.21 -0.1 359,547 - GFF1044 0.21 -0.3 359,547 - GFF1044 0.21 -0.1 359,547 - GFF1044 0.21 +1.8 359,547 - GFF1044 0.21 -0.4 359,547 - GFF1044 0.21 -0.4 359,724 - GFF1044 0.66 -0.2 359,724 - GFF1044 0.66 -0.0 359,735 + GFF1044 0.69 +0.2 359,735 + GFF1044 0.69 -0.6 359,736 - GFF1044 0.69 +1.0 360,168 + GFF1045 0.15 -0.2 360,168 + GFF1045 0.15 -0.6 360,168 + GFF1045 0.15 +0.4 360,168 + GFF1045 0.15 +1.0 360,169 - GFF1045 0.15 +1.5 360,169 - GFF1045 0.15 +0.1 360,169 - GFF1045 0.15 -0.9 360,169 - GFF1045 0.15 +0.2 360,169 - GFF1045 0.15 +1.4 360,169 - GFF1045 0.15 -1.0 360,169 - GFF1045 0.15 -0.0 360,169 - GFF1045 0.15 +0.6 360,282 + GFF1045 0.21 -0.5
Or see this region's nucleotide sequence