Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1017 and GFF1018 are separated by 33 nucleotides GFF1018 and GFF1019 are separated by 7 nucleotides GFF1019 and GFF1020 are separated by 290 nucleotides GFF1020 and GFF1021 are separated by 104 nucleotides
GFF1017 - conserved hypothetical protein-transmembrane prediction, at 334,966 to 335,946
GFF1017
GFF1018 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase (EC 2.1.1.185), at 335,980 to 336,726
GFF1018
GFF1019 - monooxygenase, FAD-binding, at 336,734 to 337,759
GFF1019
GFF1020 - hypothetical protein, at 338,050 to 338,220
GFF1020
GFF1021 - Transcriptional regulator, Xre-family with cupin domain, at 338,325 to 338,930
GFF1021
Position (kb)
336
337
338 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 335.891 kb on + strand at 335.892 kb on - strand at 335.965 kb on - strand at 335.965 kb on - strand at 335.965 kb on - strand at 335.970 kb on + strand at 335.971 kb on - strand at 336.042 kb on + strand at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.539 kb on - strand, within GFF1018 at 336.539 kb on - strand, within GFF1018 at 336.697 kb on + strand at 336.697 kb on + strand at 336.697 kb on + strand at 336.698 kb on - strand at 336.698 kb on - strand at 336.735 kb on + strand at 336.735 kb on + strand at 336.735 kb on + strand at 336.736 kb on - strand at 336.736 kb on - strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.894 kb on + strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 337.254 kb on + strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.320 kb on + strand, within GFF1019 at 337.320 kb on + strand, within GFF1019 at 337.321 kb on - strand, within GFF1019 at 337.379 kb on + strand, within GFF1019 at 337.380 kb on - strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.620 kb on + strand, within GFF1019 at 337.620 kb on + strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.751 kb on + strand at 337.752 kb on - strand at 337.752 kb on - strand at 337.752 kb on - strand at 337.752 kb on - strand at 337.996 kb on + strand at 337.996 kb on + strand at 338.046 kb on + strand at 338.047 kb on - strand at 338.132 kb on + strand, within GFF1020 at 338.132 kb on + strand, within GFF1020 at 338.132 kb on + strand, within GFF1020 at 338.132 kb on + strand, within GFF1020 at 338.132 kb on + strand, within GFF1020 at 338.133 kb on - strand, within GFF1020 at 338.133 kb on - strand, within GFF1020 at 338.133 kb on - strand, within GFF1020 at 338.133 kb on - strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.187 kb on + strand, within GFF1020 at 338.188 kb on - strand, within GFF1020 at 338.188 kb on - strand, within GFF1020 at 338.204 kb on + strand at 338.204 kb on + strand at 338.204 kb on + strand at 338.204 kb on + strand at 338.204 kb on + strand at 338.204 kb on + strand at 338.204 kb on + strand at 338.205 kb on - strand at 338.205 kb on - strand at 338.205 kb on - strand at 338.205 kb on - strand at 338.254 kb on + strand at 338.255 kb on - strand at 338.255 kb on - strand at 338.255 kb on - strand at 338.315 kb on + strand at 338.316 kb on - strand at 338.327 kb on - strand at 338.449 kb on + strand, within GFF1021 at 338.449 kb on + strand, within GFF1021 at 338.450 kb on - strand, within GFF1021 at 338.450 kb on - strand, within GFF1021 at 338.450 kb on - strand, within GFF1021 at 338.518 kb on + strand, within GFF1021 at 338.519 kb on - strand, within GFF1021 at 338.519 kb on - strand, within GFF1021 at 338.605 kb on + strand, within GFF1021 at 338.605 kb on + strand, within GFF1021 at 338.605 kb on + strand, within GFF1021 at 338.605 kb on + strand, within GFF1021 at 338.606 kb on - strand, within GFF1021
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 335,891 + +1.4 335,892 - -0.6 335,965 - -0.4 335,965 - +1.4 335,965 - -0.5 335,970 + -0.6 335,971 - -0.4 336,042 + -1.6 336,442 + GFF1018 0.62 -1.8 336,442 + GFF1018 0.62 +0.3 336,442 + GFF1018 0.62 -2.1 336,442 + GFF1018 0.62 -0.8 336,442 + GFF1018 0.62 +0.2 336,442 + GFF1018 0.62 -0.6 336,443 - GFF1018 0.62 +0.4 336,443 - GFF1018 0.62 -0.8 336,443 - GFF1018 0.62 -0.3 336,443 - GFF1018 0.62 +0.1 336,443 - GFF1018 0.62 -0.4 336,534 + GFF1018 0.74 +0.5 336,534 + GFF1018 0.74 -0.3 336,534 + GFF1018 0.74 +0.4 336,535 - GFF1018 0.74 +0.4 336,535 - GFF1018 0.74 +0.4 336,535 - GFF1018 0.74 +0.2 336,535 - GFF1018 0.74 -0.8 336,535 - GFF1018 0.74 +1.6 336,535 - GFF1018 0.74 +0.3 336,535 - GFF1018 0.74 -1.1 336,535 - GFF1018 0.74 +0.9 336,538 + GFF1018 0.75 +0.2 336,538 + GFF1018 0.75 +0.3 336,538 + GFF1018 0.75 -2.0 336,538 + GFF1018 0.75 -0.6 336,538 + GFF1018 0.75 -1.8 336,538 + GFF1018 0.75 +1.7 336,538 + GFF1018 0.75 -0.8 336,538 + GFF1018 0.75 -2.0 336,538 + GFF1018 0.75 -0.1 336,539 - GFF1018 0.75 -0.5 336,539 - GFF1018 0.75 -0.2 336,697 + -0.4 336,697 + +0.2 336,697 + +0.0 336,698 - -0.1 336,698 - -0.2 336,735 + -0.1 336,735 + +0.0 336,735 + +0.6 336,736 - -0.9 336,736 - +2.0 336,777 + -1.0 336,777 + -1.3 336,777 + +0.9 336,777 + +0.8 336,777 + -0.1 336,777 + -1.5 336,777 + -2.0 336,778 - -0.2 336,778 - +0.3 336,778 - -1.4 336,778 - +0.3 336,894 + GFF1019 0.16 +0.8 336,895 - GFF1019 0.16 -0.0 336,895 - GFF1019 0.16 +1.0 336,895 - GFF1019 0.16 +0.2 337,254 + GFF1019 0.51 -0.1 337,255 - GFF1019 0.51 -0.6 337,255 - GFF1019 0.51 +0.4 337,255 - GFF1019 0.51 +0.1 337,255 - GFF1019 0.51 +0.4 337,320 + GFF1019 0.57 +1.8 337,320 + GFF1019 0.57 +0.4 337,321 - GFF1019 0.57 +0.6 337,379 + GFF1019 0.63 -0.5 337,380 - GFF1019 0.63 +0.9 337,413 + GFF1019 0.66 +0.0 337,413 + GFF1019 0.66 +1.2 337,413 + GFF1019 0.66 -0.2 337,413 + GFF1019 0.66 -0.5 337,413 + GFF1019 0.66 -0.1 337,414 - GFF1019 0.66 +1.0 337,414 - GFF1019 0.66 -0.6 337,414 - GFF1019 0.66 -0.6 337,414 - GFF1019 0.66 +0.6 337,414 - GFF1019 0.66 -0.4 337,414 - GFF1019 0.66 -0.2 337,414 - GFF1019 0.66 +0.9 337,414 - GFF1019 0.66 -0.1 337,620 + GFF1019 0.86 -2.0 337,620 + GFF1019 0.86 -1.0 337,621 - GFF1019 0.86 -0.6 337,621 - GFF1019 0.86 +0.8 337,621 - GFF1019 0.86 +0.5 337,646 + GFF1019 0.89 -2.1 337,646 + GFF1019 0.89 -0.1 337,646 + GFF1019 0.89 +0.6 337,646 + GFF1019 0.89 -2.0 337,751 + +0.1 337,752 - -0.4 337,752 - +0.7 337,752 - -0.8 337,752 - +0.6 337,996 + +0.0 337,996 + +0.7 338,046 + -0.7 338,047 - +0.5 338,132 + GFF1020 0.48 -0.4 338,132 + GFF1020 0.48 +0.8 338,132 + GFF1020 0.48 +0.1 338,132 + GFF1020 0.48 -0.0 338,132 + GFF1020 0.48 +0.4 338,133 - GFF1020 0.49 -0.2 338,133 - GFF1020 0.49 +0.5 338,133 - GFF1020 0.49 -0.5 338,133 - GFF1020 0.49 -0.5 338,187 + GFF1020 0.80 -1.2 338,187 + GFF1020 0.80 -0.0 338,187 + GFF1020 0.80 +0.0 338,187 + GFF1020 0.80 +0.2 338,187 + GFF1020 0.80 +0.4 338,187 + GFF1020 0.80 -0.7 338,187 + GFF1020 0.80 -0.2 338,188 - GFF1020 0.81 -0.2 338,188 - GFF1020 0.81 +0.4 338,204 + +0.2 338,204 + +0.4 338,204 + -1.2 338,204 + +1.1 338,204 + -0.0 338,204 + +0.2 338,204 + -0.2 338,205 - -0.4 338,205 - +0.4 338,205 - -2.2 338,205 - -0.5 338,254 + -0.2 338,255 - -0.4 338,255 - +0.3 338,255 - +0.7 338,315 + -0.6 338,316 - -1.0 338,327 - -0.1 338,449 + GFF1021 0.20 +0.1 338,449 + GFF1021 0.20 -1.1 338,450 - GFF1021 0.21 +0.4 338,450 - GFF1021 0.21 -0.2 338,450 - GFF1021 0.21 +0.0 338,518 + GFF1021 0.32 -2.1 338,519 - GFF1021 0.32 -0.4 338,519 - GFF1021 0.32 -0.3 338,605 + GFF1021 0.46 -2.2 338,605 + GFF1021 0.46 +0.0 338,605 + GFF1021 0.46 -0.2 338,605 + GFF1021 0.46 -1.2 338,606 - GFF1021 0.46 -0.4
Or see this region's nucleotide sequence